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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0538

PubMed

23976882, PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.

Characterization method

RNA-Seq

Genomic accession number

CP012801.1

Nucelotide position range

2388865-2412814

Substrate

galactomannan,glucomannan,cellobiose,xyloglucan

Loci

BcellWH2_02020-BcellWH2_02034

Species

Bacteroides cellulosilyticus/246787

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BcellWH2_02020 ALJ59265.1 0 - 792 (-) CP012801.1:2388865-2389657 -
bfce BcellWH2_02021 ALJ59266.1 810 - 1998 (-) CP012801.1:2389675-2390863 5.1.3.11
yicJ_1 BcellWH2_02022 ALJ59267.1 2391 - 3771 (-) CP012801.1:2391256-2392636 -
- BcellWH2_02023 ALJ59268.1 3845 - 5024 (-) CP012801.1:2392710-2393889 2.4.1.281
manA_1 BcellWH2_02024 ALJ59269.1 5039 - 6167 (-) CP012801.1:2393904-2395032 3.2.1.78
manA_2 BcellWH2_02025 ALJ59270.1 6326 - 8003 (-) CP012801.1:2395191-2396868 3.2.1.78
- BcellWH2_02026 ALJ59271.1 8022 - 10449 (-) CP012801.1:2396887-2399314 -
- BcellWH2_02027 ALJ59272.1 10469 - 11813 (-) CP012801.1:2399334-2400678 -
- BcellWH2_02028 ALJ59273.1 11833 - 13564 (-) CP012801.1:2400698-2402429 -
- BcellWH2_02029 ALJ59274.1 13583 - 16787 (-) CP012801.1:2402448-2405652 -
eglS BcellWH2_02030 ALJ59275.1 16950 - 17931 (-) CP012801.1:2405815-2406796 3.2.1.4
- BcellWH2_02031 ALJ59276.1 17935 - 19222 (-) CP012801.1:2406800-2408087 -
axe7A_1 BcellWH2_02032 ALJ59277.1 19248 - 20532 (-) CP012801.1:2408113-2409397 3.1.1.-
btr_2 BcellWH2_02033 ALJ59278.1 20603 - 21491 (-) CP012801.1:2409468-2410356 -
bglX_4 BcellWH2_02034 ALJ59279.1 21670 - 23950 (-) CP012801.1:2410535-2412815 3.2.1.21

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 792 (-) CDS No
bfce 811 - 1998 (-) CDS No
yicJ_1 2392 - 3771 (-) TC: gnl|TC-DB|P75683|2.A.2.3.5 Yes
- 3846 - 5024 (-) CAZyme: GH130 Yes
manA_1 5040 - 6167 (-) CAZyme: GH26 Yes
manA_2 6327 - 8003 (-) CAZyme: GH26 Yes
- 8023 - 10449 (-) other Yes
- 10470 - 11813 (-) other Yes
- 11834 - 13564 (-) other Yes
- 13584 - 16787 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
eglS 16951 - 17931 (-) CAZyme: GH5_2|GH5 Yes
- 17936 - 19222 (-) CAZyme: GH5_7 Yes
axe7A_1 19249 - 20532 (-) CAZyme: CE7 Yes
btr_2 20604 - 21491 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
bglX_4 21671 - 23950 (-) CAZyme: GH3 Yes

PUL ID

PUL0538

PubMed

23976882, PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.

Title

Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome.

Author

McNulty NP, Wu M, Erickson AR, Pan C, Erickson BK, Martens EC, Pudlo NA, Muegge BD, Henrissat B, Hettich RL, Gordon JI

Abstract

The human gut microbiota is an important metabolic organ, yet little is known about how its individual species interact, establish dominant positions, and respond to changes in environmental factors such as diet. In this study, gnotobiotic mice were colonized with an artificial microbiota comprising 12 sequenced human gut bacterial species and fed oscillating diets of disparate composition. Rapid, reproducible, and reversible changes in the structure of this assemblage were observed. Time-series microbial RNA-Seq analyses revealed staggered functional responses to diet shifts throughout the assemblage that were heavily focused on carbohydrate and amino acid metabolism. High-resolution shotgun metaproteomics confirmed many of these responses at a protein level. One member, Bacteroides cellulosilyticus WH2, proved exceptionally fit regardless of diet. Its genome encoded more carbohydrate active enzymes than any previously sequenced member of the Bacteroidetes. Transcriptional profiling indicated that B. cellulosilyticus WH2 is an adaptive forager that tailors its versatile carbohydrate utilization strategy to available dietary polysaccharides, with a strong emphasis on plant-derived xylans abundant in dietary staples like cereal grains. Two highly expressed, diet-specific polysaccharide utilization loci (PULs) in B. cellulosilyticus WH2 were identified, one with characteristics of xylan utilization systems. Introduction of a B. cellulosilyticus WH2 library comprising >90,000 isogenic transposon mutants into gnotobiotic mice, along with the other artificial community members, confirmed that these loci represent critical diet-specific fitness determinants. Carbohydrates that trigger dramatic increases in expression of these two loci and many of the organism's 111 other predicted PULs were identified by RNA-Seq during in vitro growth on 31 distinct carbohydrate substrates, allowing us to better interpret in vivo RNA-Seq and proteomics data. These results offer insight into how gut microbes adapt to dietary perturbations at both a community level and from the perspective of a well-adapted symbiont with exceptional saccharolytic capabilities, and illustrate the value of artificial communities.