Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0144

PubMed

16199574, J Bacteriol. 2005 Oct;187(20):7038-44. doi: 10.1128/JB.187.20.7038-7044.2005.

Characterization method

enzyme activity assay,Western Blot

Genomic accession number

AP006878.1

Nucelotide position range

1552919-1569129

Substrate

chitin

Loci

TK1754-TK1765

Species

Thermococcus kodakarensis/311400

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- TK1754 BAD85943.1 0 - 2361 (-) AP006878.1:1552919-1555280 -
- TK1755 BAD85944.1 2366 - 3347 (-) AP006878.1:1555285-1556266 -
- TK1756 BAD85945.1 3347 - 4316 (-) AP006878.1:1556266-1557235 -
- TK1757 BAD85946.1 4308 - 5286 (-) AP006878.1:1557227-1558205 -
- TK1758 BAD85947.1 5282 - 6227 (-) AP006878.1:1558201-1559146 -
- TK1759 BAD85948.1 6229 - 7228 (-) AP006878.1:1559148-1560147 -
- TK1760 BAD85949.1 7274 - 9155 (-) AP006878.1:1560193-1562074 -
- TK1761 BAD85950.1 9389 - 10844 (+) AP006878.1:1562308-1563763 -
- TK1762 BAD85951.1 10840 - 11050 (+) AP006878.1:1563759-1563969 -
- TK1763 BAD85952.1 11046 - 11496 (+) AP006878.1:1563965-1564415 -
- TK1764 BAD85953.1 11517 - 12321 (-) AP006878.1:1564436-1565240 -
- TK1765 BAD85954.1 12563 - 16211 (+) AP006878.1:1565482-1569130 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2361 (-) CAZyme: GH35 Yes
- 2367 - 3347 (-) STP: STP|SIS,STP|SIS Yes
- 3348 - 4316 (-) TC: gnl|TC-DB|Q8U1J4|3.A.1.5.6 Yes
- 4309 - 5286 (-) TC: gnl|TC-DB|Q8U1J5|3.A.1.5.6 Yes
- 5283 - 6227 (-) TC: gnl|TC-DB|Q8U1J6|3.A.1.5.6 Yes
- 6230 - 7228 (-) TC: gnl|TC-DB|Q8U1J7|3.A.1.5.6 Yes
- 7275 - 9155 (-) TC: gnl|TC-DB|Q8U1J8|3.A.1.5.6 Yes
- 9390 - 10844 (+) CAZyme: GH1 Yes
- 10841 - 11050 (+) other Yes
- 11047 - 11496 (+) other Yes
- 11518 - 12321 (-) STP: STP|PIG-L Yes
- 12564 - 16211 (+) CAZyme: CBM2|GH18 Yes

PUL ID

PUL0144

PubMed

16199574, J Bacteriol. 2005 Oct;187(20):7038-44. doi: 10.1128/JB.187.20.7038-7044.2005.

Title

Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon.

Author

Tanaka T, Takahashi F, Fukui T, Fujiwara S, Atomi H, Imanaka T

Abstract

A key step in amino sugar metabolism is the interconversion between fructose-6-phosphate (Fru6P) and glucosamine-6-phosphate (GlcN6P). This conversion is catalyzed in the catabolic and anabolic directions by GlcN6P deaminase and GlcN6P synthase, respectively, two enzymes that show no relationship with one another in terms of primary structure. In this study, we examined the catalytic properties and regulatory features of the glmD gene product (GlmD(Tk)) present within a chitin degradation gene cluster in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. Although the protein GlmD(Tk) was predicted as a probable sugar isomerase related to the C-terminal sugar isomerase domain of GlcN6P synthase, the recombinant GlmD(Tk) clearly exhibited GlcN6P deaminase activity, generating Fru6P and ammonia from GlcN6P. This enzyme also catalyzed the reverse reaction, the ammonia-dependent amination/isomerization of Fru6P to GlcN6P, whereas no GlcN6P synthase activity dependent on glutamine was observed. Kinetic analyses clarified the preference of this enzyme for the deaminase reaction rather than the reverse one, consistent with the catabolic function of GlmD(Tk). In T. kodakaraensis cells, glmD(Tk) was polycistronically transcribed together with upstream genes encoding an ABC transporter and a downstream exo-beta-glucosaminidase gene (glmA(Tk)) within the gene cluster, and their expression was induced by the chitin degradation intermediate, diacetylchitobiose. The results presented here indicate that GlmD(Tk) is actually a GlcN6P deaminase functioning in the entry of chitin-derived monosaccharides to glycolysis in this hyperthermophile. This enzyme is the first example of an archaeal GlcN6P deaminase and is a structurally novel type distinct from any previously known GlcN6P deaminase.