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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0376

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

3502745-3522095

Substrate

mucin,O-glycan

Loci

BT_2818-BT_2826

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_2818 AAO77924.1 0 - 3174 (+) AE015928.1:3502745-3505919 -
- BT_2819 AAO77925.1 3185 - 4943 (+) AE015928.1:3505930-3507688 -
- BT_2820 AAO77926.1 4972 - 7753 (+) AE015928.1:3507717-3510498 -
- BT_2821 AAO77927.1 7775 - 9497 (+) AE015928.1:3510520-3512242 -
- BT_2822 AAO77928.1 9528 - 11007 (+) AE015928.1:3512273-3513752 -
- BT_2823 AAO77929.1 11034 - 12510 (+) AE015928.1:3513779-3515255 -
- BT_2824 AAO77930.1 12606 - 13725 (+) AE015928.1:3515351-3516470 -
- BT_2825 AAO77931.1 13850 - 14894 (+) AE015928.1:3516595-3517639 -
- BT_2826 AAO77932.1 15433 - 19351 (-) AE015928.1:3518178-3522096 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3174 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 3186 - 4943 (+) other Yes
- 4973 - 7753 (+) TC: gnl|TC-DB|Q93TH9|1.B.14.6.2 Yes
- 7776 - 9497 (+) other Yes
- 9529 - 11007 (+) other Yes
- 11035 - 12510 (+) other Yes
- 12607 - 13725 (+) CAZyme: GH16 Yes
- 13851 - 14894 (+) CAZyme: GH18 Yes
- 15434 - 19351 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 No

PUL ID

PUL0376

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.