Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0002 enzyme activity assay, Northern Blot lichenan Bacillus subtilis 8606172
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J Bacteriol. 1996 Apr;178(7):1971-9. doi: 10.1128/jb.178.7.1971-1979.1996.
1996 Apr degradation 2 1 GH16
PUL0049 fosmid library screen beta-glucan, galactomannan feces metagenome 29601586
Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018.
2018 degradation 29 6 GH43_17, GH26, GH16
PUL0118 qRT-PCR, affinity gel electrophoresis, isothermal titration calorimetry laminarin, beta-glucan Bacteroides uniformis 32265336
Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides. mBio. 2020 Apr 7;11(2). pii: mBio.00095-20. doi: 10.1128/mBio.00095-20.
2020 Apr 7 degradation 7 3 CBM6, GH3, GH158, GH16
PUL0125 affinity gel electrophoresis, carbohydrate binding assay, RNA-seq beta-glucan Bacteroides ovatus 31062073, 32801182
Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Mol Life Sci. 2019 Nov;76(21):4319-4340. doi: 10.1007/s00018-019-03115-3. Epub 2019 May 6. Appl Environ Microbiol. 2020 Oct 1;86(20). pii: AEM.01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2019 Nov,2020 Oct 1 degradation 6 2 GH16, GH3
PUL0146 sequence homology analysis carrageenan Pseudoalteromonas atlantica 30524390
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018.
2018 degradation 23 1 GH16
PUL0147 sequence homology analysis carrageenan Pseudoalteromonas carrageenovora 30524390
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018.
2018 degradation 30 5 GH16, GH16, GH150
PUL0170 enzyme activity assay beta-glucan Bacteroides ovatus 29141231
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Cell Rep. 2017 Nov 14;21(7):2030. doi: 10.1016/j.celrep.2017.11.013.
2017 Nov 14 degradation 7 3 GH3, GH16, GH3
PUL0187 qRT-PCR, enzyme activity assay beta-glucan Paenibacillus sp. JDR-2 26746717
A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2. Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.
2016 Jan 8 degradation 7 2 CBM4, CBM54, CBM6, GH16, GH16
PUL0220 mass spectrometry, target decoy database analysis laminarin Polaribacter sp. Hel1_33_49 25478683
Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015 Jun;9(6):1410-22. doi: 10.1038/ismej.2014.225. Epub 2014 Dec 5.
2015 Jun degradation 11 5 GH30, GH30_1, GH17, GH3, GH149, GH16
PUL0225 RT-PCR porphyran Bacteroides plebeius 23150581
Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19786-91. doi: 10.1073/pnas.1211002109. Epub 2012 Nov 12.
2012 Nov 27 degradation 36 12 GH16, GH117, GH2, GH2, GH50, GH154, GH105, GH16, GH86, GH86, GH29, GH16, GH2
PUL0234 proteome fractionation, mass spectrometry, target decoy database analysis laminarin Gramella forsetii 24522261
Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13.
2014 Jul degradation 7 3 GH16, GH3, GH16
PUL0264 RNA-Seq carrageenan Pseudoalteromonas distincta 31886414
Insights into the kappa/iota-carrageenan metabolism pathway of some marine Pseudoalteromonas species. Commun Biol. 2019 Dec 19;2:474. doi: 10.1038/s42003-019-0721-y. eCollection 2019.
2019 degradation 29 5 GH16, GH16
PUL0292 enzyme activity assay chitin Collimonas fungivorans 18671744
Identification and characterization of genes underlying chitinolysis in Collimonas fungivorans Ter331. FEMS Microbiol Ecol. 2008 Oct;66(1):123-35. doi: 10.1111/j.1574-6941.2008.00547.x. Epub 2008 Jul 30.
2008 Oct degradation 14 3 GH0, GH16, GH3
PUL0308 microarray, enzyme activity assay, high performance anion exchange chromatography, mass spectrometry, RNA-seq beta-glucan Bacteroides ovatus 29020628, 22205877, 32801182
Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Sharing a beta-Glucan Meal: Transcriptomic Eavesdropping on a Bacteroides ovatus-Subdoligranulum variabile-Hungatella hathewayi Consortium. Cell Rep. 2017 Oct 10;21(2):417-430. doi: 10.1016/j.celrep.2017.09.049. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20. Appl Environ Microbiol. 2020 Oct 1;86(20). pii: AEM.01651-20. doi: 10.1128/AEM.01651-20. Print 2020 Oct 1.
2017 Oct 10,2011 Dec,2020 Oct 1 degradation 5 3 GH16, GH3
PUL0315 microarray agar, porphyran Zobellia galactanivorans 28983288
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017.
2017 degradation 4 2 GH16
PUL0317 microarray agar, porphyran Zobellia galactanivorans 28983288
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017.
2017 degradation 3 2 GH16
PUL0318 microarray carrageenan Zobellia galactanivorans 28983288
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017.
2017 degradation 3 1 CBM16, GH16
PUL0335 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 31 4 GH13, GH97, GH3, GH158, GH16
PUL0336 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 25 3 GH3, GH158, GH16
PUL0337 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 29 3 GH16, GH158, GH3
PUL0338 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 34 4 GH16, GH158, GH3, GH97
PUL0339 fosmid library screen, enzyme activity assay, thin layer chromatography xylan, beta-glucan, lichenan uncultured bacterium 28091525
A fibrolytic potential in the human ileum mucosal microbiota revealed by functional metagenomic. Sci Rep. 2017 Jan 16;7:40248. doi: 10.1038/srep40248.
2017 Jan 16 degradation 24 4 GH97, GH3, GH16, GH20
PUL0346 gene deletion mutant and growth assay xylooligosaccharide uncultured bacterium 27573446
Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli. Mol Microbiol. 2016 Nov;102(4):579-592. doi: 10.1111/mmi.13480. Epub 2016 Sep 14.
2016 Nov degradation 15 5 GH43, GH43_29, GH43, GH43_12, GH43, GH43_1, GH10, GH16
PUL0376 microarray, qPCR mucin, O-glycan Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 9 2 GH16, GH18
PUL0393 enzyme activity assay, analysis of reaction products galactan Microbulbifer thermotolerans 20686828
Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94T, and insight into the unusual catalytic mechanism. Mar Biotechnol (NY). 2011 Jun;13(3):411-22. doi: 10.1007/s10126-010-9312-0. Epub 2010 Aug 5.
2011 Jun degradation 10 1 GH86, CBM6, GH16, CBM6
PUL0401 RNA-Seq laminarin Alteromonas macleodii 30116038
Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16.
2019 Jan degradation 9 3 CBM4, GH16, GH3
PUL0406 high performance anion exchange chromatography beta-glucan Coprothermobacter proteolyticus 30315317
From proteins to polysaccharides: lifestyle and genetic evolution of Coprothermobacter proteolyticus. ISME J. 2019 Mar;13(3):603-617. doi: 10.1038/s41396-018-0290-y. Epub 2018 Oct 12.
2019 Mar degradation 20 3 GH16, GH3, GH18
PUL0435 mass spectrometry, high performance anion exchange chromatography glucose Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 13 4 GH43, GH43_34, GH30, GH30_1, GH30, GH30_3, GH16
PUL0442 mass spectrometry, high performance anion exchange chromatography glucomannan, galactomannan, glucose Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 9 3 CBM5, GH18, GH18, GH64, CBM6, GH16, CBM6
PUL0443 mass spectrometry, high performance anion exchange chromatography glucomannan, galactomannan, glucose Chitinophaga pinensis 28069559
Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis. J Proteomics. 2017 Mar 6;156:63-74. doi: 10.1016/j.jprot.2017.01.003. Epub 2017 Jan 6.
2017 Mar 6 degradation 9 3 GH16, CBM6
PUL0458 RNA-Seq, analysis of reaction products, enzyme activity assay carrageenan Colwellia echini 31915221
A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. mSphere. 2020 Jan 8;5(1). pii: 5/1/e00792-19. doi: 10.1128/mSphere.00792-19.
2020 Jan 8 degradation 49 12 GH16, GH16, CBM16, GH16, GH16
PUL0460 recombinant protein expression, RT-PCR, enzyme activity assay carrageenan, agar Paraglaciecola hydrolytica 29774012
A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.
2018 degradation 108 18 GH2, GH29, GH63, GH86, CBM6, GH50, GH86, GH50, GH117, GH50, GH16, GH50, CE1, GH16, GH127, GH16, GH82, GH16, GH16, GH16
PUL0477 growth assay beta-glucan Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 6 2 GH16, GH16
PUL0480 growth assay hemicellulose Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 9 4 GH3, GH10, GH16, GH8
PUL0528 microarray, qPCR beta-glucan Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH3, GH16, GH3
PUL0535 RNA-Seq arabinogalactan Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 9 2 GH16, GH43, GH43_24, GH16
PUL0551 RNA-Seq cellobiose, beta-glucan, glucomannan, laminarin Bacteroides cellulosilyticus 23976882
Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20.
2013 degradation 6 2 GH16, GH3
PUL0563 microarray, qPCR arabinogalactan Bacteroides thetaiotaomicron 18996345, 22205877
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2008 Nov 13,2011 Dec degradation 14 7 GH127, GH43, GH43_34, GH154, GH43, GH16, GH43_24, GH43_24, GH145, GH105
PUL0607 enzymy activity assay, clone and expression, liquid chromatography and mass spectrometry porphyran Wenyingzhuangia fucanilytica strain CZ1127 32520542
Characterization of a Novel Porphyranase Accommodating Methyl-galactoses at Its Subsites. J Agric Food Chem. 2020 Jul 1;68(26):7032-7039. doi: 10.1021/acs.jafc.0c02404. Epub 2020 Jun 22.
2020 Jul 1 degradation 22 8 GH2, CBM67, CBM51, GH141, PL0, GH105, GH154, GH16_11, GH16, GH117, GH16_11, GH16, GH16_14, GH29