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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0292

PubMed

18671744, FEMS Microbiol Ecol. 2008 Oct;66(1):123-35. doi: 10.1111/j.1574-6941.2008.00547.x. Epub 2008 Jul 30.

Characterization method

enzyme activity assay

Genomic accession number

EU599185.1

Nucelotide position range

495-16938

Substrate

chitin

Loci

ACF93782.1-ACF93795.1

Species

Collimonas fungivorans/158899

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

chiI - ACF93782.1 495 - 1740 (+) EU599185.1:990-2235 3.2.1.14
- - ACF93783.1 1846 - 2182 (-) EU599185.1:2341-2677 -
chiII - ACF93784.1 2352 - 3513 (-) EU599185.1:2847-4008 3.2.1.14
- - ACF93785.1 3530 - 4451 (-) EU599185.1:4025-4946 -
ampG - ACF93786.1 4456 - 5758 (-) EU599185.1:4951-6253 -
hexI - ACF93787.1 5754 - 7320 (-) EU599185.1:6249-7815 3.2.1.52
nagB1 - ACF93788.1 7377 - 8388 (-) EU599185.1:7872-8883 3.5.99.6
nagQ - ACF93789.1 9558 - 10293 (-) EU599185.1:10053-10788 -
nagK1 - ACF93790.1 10286 - 11261 (-) EU599185.1:10781-11756 2.7.1.59
exbD2 - ACF93791.1 11399 - 11834 (-) EU599185.1:11894-12329 -
exbD1 - ACF93792.1 11951 - 12380 (-) EU599185.1:12446-12875 -
exbB - ACF93793.1 12382 - 13303 (-) EU599185.1:12877-13798 -
- - ACF93794.1 13380 - 14031 (-) EU599185.1:13875-14526 -
- - ACF93795.1 14265 - 16938 (-) EU599185.1:14760-17433 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 496 - 1740 (+) CDS No
- 1847 - 2182 (-) CDS No
- 2353 - 3513 (-) CAZyme: GH0|GH16 Yes
- 3531 - 4451 (-) other Yes
- 4457 - 5758 (-) other Yes
- 5755 - 7320 (-) CAZyme: GH3 Yes
- 7378 - 8388 (-) STP: STP|SIS,STP|SIS Yes
- 9559 - 10293 (-) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0039384 Yes
- 10287 - 11261 (-) other Yes
- 11400 - 11834 (-) TC: gnl|TC-DB|Q1D0D2|1.A.30.2.7 Yes
- 11952 - 12380 (-) TC: gnl|TC-DB|Q1D0D2|1.A.30.2.7 Yes
- 12383 - 13303 (-) TC: gnl|TC-DB|Q9A608|1.B.14.12.1 Yes
- 13381 - 14031 (-) CDS No
- 14266 - 16938 (-) CDS No

PUL ID

PUL0292

PubMed

18671744, FEMS Microbiol Ecol. 2008 Oct;66(1):123-35. doi: 10.1111/j.1574-6941.2008.00547.x. Epub 2008 Jul 30.

Title

Identification and characterization of genes underlying chitinolysis in Collimonas fungivorans Ter331.

Author

Fritsche K, de Boer W, Gerards S, van den Berg M, van Veen JA, Leveau JH

Abstract

Through a combinatorial approach of plasposon mutagenesis, genome mining, and heterologous expression, we identified genes contributing to the chitinolytic phenotype of bacterium Collimonas fungivorans Ter331. One of five mutants with abolished ability to hydrolyze colloidal chitin carried its plasposon in the chiI gene coding for an extracellular endochitinase. Two mutants were affected in the promoter of chiP-II coding for an outer-membrane transporter of chitooligosaccharides. The remaining two mutations were linked to chitobiose/N-acetylglucosamine uptake. Thus, our model for the Collimonas chitinolytic system assumes a positive feedback regulation of chitinase activity by chitin degradation products. A second chitinase gene, chiII, coded for an exochitinase that preferentially released chitobiose from chitin analogs. Genes hexI and hexII showed coding resemblance to N-acetylglucosaminidases, and the activity of purified HexI protein towards chitin analogs suggested its role in converting chitobiose to N-acetylglucosamine. The hexI gene clustered with chiI, chiII, and chiP-II in one locus, while chitobiose/N-acetylglucosamine uptake genes colocalized in another. Both loci contained genes for conversion of N-acetylglucosamine to fructose-6-phosphate, confirming that C. fungivorans Ter331 features a complete chitin pathway. No link could be established between chitinolysis and antifungal activity of C. fungivorans Ter331, suggesting that the bacterium's reported antagonism towards fungi relies on other mechanisms.