dbCAN-PUL

PUL ID	PUL0460
PubMed	29774012, Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.
Characterization method	recombinant protein expression,RT-PCR,enzyme activity assay
Genomic accession number	NZ_LSNE01000005.1
Nucelotide position range	77050-234402
Substrate	carrageenan,agar
Loci	AX660_RS08390-AX660_RS08930
Species	Paraglaciecola hydrolytica/1799789
Degradation or Biosynthesis	degradation

Gene Name	Locus Tag	Protein ID	Gene Position	GenBank Contig Range	EC Number
-	AX660_RS08390	WP_068376952.1	77050 - 79585 (+)	NZ_LSNE01000005.1:154100-156635	-
-	AX660_RS08395	WP_106404062.1	79639 - 81214 (+)	NZ_LSNE01000005.1:156689-158264	-
-	AX660_RS08400	WP_082768813.1	81456 - 82737 (-)	NZ_LSNE01000005.1:158506-159787	-
-	AX660_RS08405	WP_068375542.1	82651 - 83638 (-)	NZ_LSNE01000005.1:159701-160688	-
-	AX660_RS08410	WP_068375545.1	83668 - 84958 (-)	NZ_LSNE01000005.1:160718-162008	-
-	AX660_RS08415	WP_068375547.1	85160 - 87608 (+)	NZ_LSNE01000005.1:162210-164658	-
-	AX660_RS08420	WP_068375550.1	87776 - 89354 (+)	NZ_LSNE01000005.1:164826-166404	-
-	AX660_RS08425	WP_068375554.1	89369 - 90635 (+)	NZ_LSNE01000005.1:166419-167685	-
-	AX660_RS08430	WP_068375556.1	90690 - 91848 (+)	NZ_LSNE01000005.1:167740-168898	-
-	AX660_RS08435	WP_068375558.1	91998 - 93105 (-)	NZ_LSNE01000005.1:169048-170155	-
-	AX660_RS08440	WP_162266408.1	93171 - 93524 (-)	NZ_LSNE01000005.1:170221-170574	-
-	AX660_RS08445	WP_162266409.1	93657 - 94087 (-)	NZ_LSNE01000005.1:170707-171137	-
-	AX660_RS23290	WP_068375562.1	94989 - 95979 (+)	NZ_LSNE01000005.1:172039-173029	-
-	AX660_RS22805	WP_131811792.1	96118 - 96282 (+)	NZ_LSNE01000005.1:173168-173332	-
-	AX660_RS08455	WP_162266410.1	96463 - 97699 (+)	NZ_LSNE01000005.1:173513-174749	-
-	AX660_RS08460	WP_068375565.1	97980 - 101256 (+)	NZ_LSNE01000005.1:175030-178306	-
-	AX660_RS08465	WP_068375567.1	101362 - 102868 (+)	NZ_LSNE01000005.1:178412-179918	-
-	AX660_RS08470	WP_068375568.1	102871 - 104347 (+)	NZ_LSNE01000005.1:179921-181397	-
-	AX660_RS08475	WP_068375570.1	104660 - 105443 (+)	NZ_LSNE01000005.1:181710-182493	-
-	AX660_RS08480	WP_068375572.1	105996 - 110943 (+)	NZ_LSNE01000005.1:183046-187993	-
-	AX660_RS08485	WP_068375574.1	110966 - 111542 (+)	NZ_LSNE01000005.1:188016-188592	-
-	AX660_RS08490	WP_068375576.1	111672 - 112287 (-)	NZ_LSNE01000005.1:188722-189337	-
-	AX660_RS08495	WP_157884330.1	112638 - 114957 (-)	NZ_LSNE01000005.1:189688-192007	-
-	AX660_RS08500	WP_068375580.1	115330 - 116434 (+)	NZ_LSNE01000005.1:192380-193484	-
-	AX660_RS08505	WP_157884331.1	116445 - 117010 (+)	NZ_LSNE01000005.1:193495-194060	-
-	AX660_RS08510	WP_157884332.1	117144 - 117599 (+)	NZ_LSNE01000005.1:194194-194649	-
-	AX660_RS08515	WP_068376956.1	117622 - 118387 (+)	NZ_LSNE01000005.1:194672-195437	1.1.1.127
aldA	AX660_RS08520	WP_068375588.1	118392 - 119871 (+)	NZ_LSNE01000005.1:195442-196921	-
-	AX660_RS08525	WP_068375590.1	120098 - 121703 (+)	NZ_LSNE01000005.1:197148-198753	-
-	AX660_RS08530	WP_082768815.1	121750 - 123607 (+)	NZ_LSNE01000005.1:198800-200657	-
-	AX660_RS08535	WP_068375592.1	123750 - 124293 (-)	NZ_LSNE01000005.1:200800-201343	-
-	AX660_RS08540	WP_068375594.1	124438 - 124876 (+)	NZ_LSNE01000005.1:201488-201926	-
-	AX660_RS08545	WP_082768817.1	124875 - 125790 (+)	NZ_LSNE01000005.1:201925-202840	-
-	AX660_RS08550	WP_068375596.1	125901 - 127371 (-)	NZ_LSNE01000005.1:202951-204421	-
-	AX660_RS08555	WP_068376965.1	127666 - 128623 (+)	NZ_LSNE01000005.1:204716-205673	-
-	AX660_RS08560	WP_068375598.1	128656 - 128935 (+)	NZ_LSNE01000005.1:205706-205985	-
-	AX660_RS08565	WP_068375600.1	128931 - 129234 (+)	NZ_LSNE01000005.1:205981-206284	-
-	AX660_RS08570	-	129324 - 129818 (-)	NZ_LSNE01000005.1:206374-206868	-
-	AX660_RS08575	WP_068375602.1	129958 - 131789 (-)	NZ_LSNE01000005.1:207008-208839	-
-	AX660_RS08580	WP_082768862.1	131812 - 134212 (-)	NZ_LSNE01000005.1:208862-211262	-
-	AX660_RS08585	WP_068375606.1	134459 - 135074 (-)	NZ_LSNE01000005.1:211509-212124	-
-	AX660_RS08590	WP_068375608.1	135180 - 137496 (-)	NZ_LSNE01000005.1:212230-214546	-
-	AX660_RS08595	WP_068375609.1	137946 - 141609 (+)	NZ_LSNE01000005.1:214996-218659	-
-	AX660_RS08600	WP_068375612.1	141669 - 143817 (+)	NZ_LSNE01000005.1:218719-220867	-
-	AX660_RS08605	WP_106404052.1	144101 - 144812 (+)	NZ_LSNE01000005.1:221151-221862	-
-	AX660_RS08610	WP_068375616.1	144825 - 145470 (+)	NZ_LSNE01000005.1:221875-222520	-
-	AX660_RS08615	WP_068375619.1	145606 - 147178 (+)	NZ_LSNE01000005.1:222656-224228	-
-	AX660_RS08620	WP_068375621.1	147208 - 148288 (+)	NZ_LSNE01000005.1:224258-225338	-
-	AX660_RS08625	WP_068375623.1	148330 - 149254 (+)	NZ_LSNE01000005.1:225380-226304	-
-	AX660_RS08630	WP_068375624.1	149253 - 150699 (+)	NZ_LSNE01000005.1:226303-227749	-
-	AX660_RS08635	WP_106404063.1	150807 - 152070 (+)	NZ_LSNE01000005.1:227857-229120	-
-	AX660_RS08640	WP_068375626.1	152059 - 152410 (+)	NZ_LSNE01000005.1:229109-229460	-
tnpA	AX660_RS08645	WP_068375627.1	152754 - 153198 (+)	NZ_LSNE01000005.1:229804-230248	-
-	AX660_RS08655	WP_068375632.1	153572 - 154451 (-)	NZ_LSNE01000005.1:230622-231501	-
-	AX660_RS08660	WP_068375633.1	154594 - 155317 (+)	NZ_LSNE01000005.1:231644-232367	-
-	AX660_RS08665	WP_068375635.1	155331 - 155682 (+)	NZ_LSNE01000005.1:232381-232732	-
-	AX660_RS08670	WP_068375637.1	156638 - 159005 (-)	NZ_LSNE01000005.1:233688-236055	-
-	AX660_RS08675	WP_164483516.1	159233 - 160241 (+)	NZ_LSNE01000005.1:236283-237291	-
-	AX660_RS08680	WP_068375639.1	160309 - 161590 (-)	NZ_LSNE01000005.1:237359-238640	-
-	AX660_RS08685	WP_068375641.1	161602 - 162133 (-)	NZ_LSNE01000005.1:238652-239183	-
-	AX660_RS08690	WP_082768818.1	162107 - 163154 (-)	NZ_LSNE01000005.1:239157-240204	-
-	AX660_RS08695	WP_082768865.1	163413 - 165177 (-)	NZ_LSNE01000005.1:240463-242227	-
-	AX660_RS08700	WP_131811775.1	165225 - 166164 (-)	NZ_LSNE01000005.1:242275-243214	-
-	AX660_RS08705	WP_068375647.1	166178 - 167060 (-)	NZ_LSNE01000005.1:243228-244110	-
-	AX660_RS08710	WP_131811793.1	167243 - 168079 (+)	NZ_LSNE01000005.1:244293-245129	-
-	AX660_RS08715	WP_068375649.1	168213 - 170514 (+)	NZ_LSNE01000005.1:245263-247564	-
-	AX660_RS08720	WP_068375651.1	170677 - 170977 (+)	NZ_LSNE01000005.1:247727-248027	-
-	AX660_RS08725	WP_068375653.1	171544 - 173827 (-)	NZ_LSNE01000005.1:248594-250877	-
-	AX660_RS08730	WP_106404065.1	173867 - 176057 (-)	NZ_LSNE01000005.1:250917-253107	-
-	AX660_RS08740	WP_068375659.1	176531 - 179147 (-)	NZ_LSNE01000005.1:253581-256197	-
-	AX660_RS08745	WP_068375661.1	179322 - 180585 (-)	NZ_LSNE01000005.1:256372-257635	-
-	AX660_RS08750	WP_068375663.1	180596 - 180905 (-)	NZ_LSNE01000005.1:257646-257955	-
-	AX660_RS08755	WP_068375665.1	180974 - 182132 (-)	NZ_LSNE01000005.1:258024-259182	-
-	AX660_RS08760	WP_068375668.1	182234 - 183035 (-)	NZ_LSNE01000005.1:259284-260085	-
-	AX660_RS08765	WP_068375670.1	183073 - 184192 (-)	NZ_LSNE01000005.1:260123-261242	-
-	AX660_RS08770	WP_068375672.1	184574 - 186137 (-)	NZ_LSNE01000005.1:261624-263187	-
-	AX660_RS08775	WP_068375674.1	186529 - 187387 (+)	NZ_LSNE01000005.1:263579-264437	-
-	AX660_RS08785	WP_106404066.1	187850 - 189296 (+)	NZ_LSNE01000005.1:264900-266346	-
-	AX660_RS08790	WP_068375678.1	189329 - 190877 (+)	NZ_LSNE01000005.1:266379-267927	-
-	AX660_RS08795	WP_068376979.1	190922 - 192383 (+)	NZ_LSNE01000005.1:267972-269433	-
-	AX660_RS08800	WP_068375680.1	192424 - 194308 (+)	NZ_LSNE01000005.1:269474-271358	-
-	AX660_RS08805	WP_068375683.1	194358 - 196341 (+)	NZ_LSNE01000005.1:271408-273391	-
-	AX660_RS08810	WP_068376982.1	196470 - 197460 (+)	NZ_LSNE01000005.1:273520-274510	-
-	AX660_RS08815	WP_068375685.1	197696 - 198215 (-)	NZ_LSNE01000005.1:274746-275265	-
-	AX660_RS23295	WP_162266411.1	198478 - 198915 (-)	NZ_LSNE01000005.1:275528-275965	-
-	AX660_RS08820	WP_131811777.1	198963 - 200436 (-)	NZ_LSNE01000005.1:276013-277486	-
-	AX660_RS08825	WP_106404067.1	201062 - 202133 (-)	NZ_LSNE01000005.1:278112-279183	-
-	AX660_RS08830	WP_082768866.1	202296 - 203280 (-)	NZ_LSNE01000005.1:279346-280330	-
-	AX660_RS08835	WP_068375692.1	203466 - 205899 (-)	NZ_LSNE01000005.1:280516-282949	-
-	AX660_RS08840	WP_068375694.1	205923 - 208275 (-)	NZ_LSNE01000005.1:282973-285325	-
-	AX660_RS08845	WP_068375697.1	208394 - 210071 (-)	NZ_LSNE01000005.1:285444-287121	-
-	AX660_RS08850	WP_068375699.1	210229 - 210946 (-)	NZ_LSNE01000005.1:287279-287996	-
-	AX660_RS08855	WP_068375702.1	210966 - 211698 (-)	NZ_LSNE01000005.1:288016-288748	-
-	AX660_RS08860	WP_162266412.1	211697 - 213089 (-)	NZ_LSNE01000005.1:288747-290139	-
-	AX660_RS08865	WP_068375704.1	213119 - 214088 (-)	NZ_LSNE01000005.1:290169-291138	-
-	AX660_RS08870	WP_068375706.1	214165 - 215926 (-)	NZ_LSNE01000005.1:291215-292976	-
-	AX660_RS08875	WP_068375710.1	215997 - 219663 (-)	NZ_LSNE01000005.1:293047-296713	-
-	AX660_RS08880	WP_068375712.1	220544 - 221630 (-)	NZ_LSNE01000005.1:297594-298680	-
-	AX660_RS08885	WP_068375714.1	221626 - 223207 (-)	NZ_LSNE01000005.1:298676-300257	-
-	AX660_RS08890	WP_068375716.1	223219 - 224506 (-)	NZ_LSNE01000005.1:300269-301556	-
-	AX660_RS08895	WP_068376991.1	224581 - 226708 (-)	NZ_LSNE01000005.1:301631-303758	-
-	AX660_RS08900	WP_068375718.1	226881 - 227382 (+)	NZ_LSNE01000005.1:303931-304432	-
-	AX660_RS08905	WP_068375720.1	227402 - 227867 (-)	NZ_LSNE01000005.1:304452-304917	-
-	AX660_RS08910	WP_068375721.1	228001 - 229075 (+)	NZ_LSNE01000005.1:305051-306125	-
-	AX660_RS08915	WP_068375723.1	229074 - 229998 (+)	NZ_LSNE01000005.1:306124-307048	-
-	AX660_RS08920	WP_082768820.1	230607 - 231657 (-)	NZ_LSNE01000005.1:307657-308707	-
-	AX660_RS08925	WP_068375726.1	232367 - 232934 (+)	NZ_LSNE01000005.1:309417-309984	-
-	AX660_RS08930	WP_082768821.1	232899 - 234402 (+)	NZ_LSNE01000005.1:309949-311452	-

Cluster number	2
Gene name	Gene position	Gene type	Found by CGCFinder?
-	77051 - 79585 (+)	CAZyme: GH2	Yes (cluster 1)
-	79640 - 81214 (+)	CAZyme: GH29	Yes (cluster 1)
-	81457 - 82737 (-)	other	Yes (cluster 1)
-	82652 - 83638 (-)	STP: STP\|PfkB	Yes (cluster 1)
-	83669 - 84958 (-)	CAZyme: GH63	Yes (cluster 1)
-	85161 - 87608 (+)	other	Yes (cluster 1)
-	87777 - 89354 (+)	TC: gnl\|TC-DB\|Q92911\|2.A.21.5.1	Yes (cluster 1)
-	89370 - 90635 (+)	other	Yes (cluster 1)
-	90691 - 91848 (+)	STP: STP\|Sigma70_r4	Yes (cluster 1)
-	91999 - 93105 (-)	other	Yes (cluster 1)
-	93172 - 93524 (-)	other	Yes (cluster 1)
-	93658 - 94087 (-)	other	Yes (cluster 1)
-	94990 - 95979 (+)	STP: STP\|HTH_18	Yes (cluster 1)
-	96119 - 96282 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0035607	Yes (cluster 1)
-	96464 - 97699 (+)	other	Yes (cluster 1)
-	97981 - 101256 (+)	TC: gnl\|TC-DB\|Q9A608\|1.B.14.12.1	Yes (cluster 1)
-	101363 - 102868 (+)	other	Yes (cluster 1)
-	102872 - 104347 (+)	other	Yes (cluster 1)
-	104661 - 105443 (+)	TF: DBD-SUPERFAMILY\|0040266	Yes (cluster 1)
-	105997 - 110943 (+)	CAZyme: GH86\|CBM6	Yes (cluster 1)
-	110967 - 111542 (+)	other	Yes (cluster 1)
-	111673 - 112287 (-)	TF: DBD-Pfam\|GntR,DBD-SUPERFAMILY\|0039384	Yes (cluster 1)
-	112639 - 114957 (-)	CAZyme: GH50	Yes (cluster 1)
-	115331 - 116434 (+)	CDS	No
-	116446 - 117010 (+)	CDS	No
-	117145 - 117599 (+)	CDS	No
-	117623 - 118387 (+)	CDS	No
-	118393 - 119871 (+)	CDS	No
-	120099 - 121703 (+)	CDS	No
-	121751 - 123607 (+)	CDS	No
-	123751 - 124293 (-)	STP: STP\|TetR_N	No
-	124439 - 124876 (+)	STP: STP\|Glyoxalase	No
-	124876 - 125790 (+)	CDS	No
-	125902 - 127371 (-)	CDS	No
-	127667 - 128623 (+)	CDS	No
-	128657 - 128935 (+)	CDS	No
-	128932 - 129234 (+)	CDS	No
-	129325 - 129818 (-)	CDS	No
-	129959 - 131789 (-)	CDS	No
-	131813 - 134212 (-)	CAZyme: GH86	Yes (cluster 2)
-	134460 - 135074 (-)	TF: DBD-Pfam\|GntR,DBD-SUPERFAMILY\|0039384	Yes (cluster 2)
-	135181 - 137496 (-)	CAZyme: GH50	Yes (cluster 2)
-	137947 - 141609 (+)	TC: gnl\|TC-DB\|Q9AAZ6\|1.B.14.12.2	Yes (cluster 2)
-	141670 - 143817 (+)	other	Yes (cluster 2)
-	144102 - 144812 (+)	other	Yes (cluster 2)
-	144826 - 145470 (+)	other	Yes (cluster 2)
-	145607 - 147178 (+)	TC: gnl\|TC-DB\|P96710\|2.A.1.1.55	Yes (cluster 2)
-	147209 - 148288 (+)	CAZyme: GH117	Yes (cluster 2)
-	148331 - 149254 (+)	STP: STP\|PfkB	Yes (cluster 2)
-	149254 - 150699 (+)	other	Yes (cluster 2)
-	150808 - 152070 (+)	other	Yes (cluster 2)
-	152060 - 152410 (+)	other	Yes (cluster 2)
-	152755 - 153198 (+)	other	Yes (cluster 2)
-	153573 - 154451 (-)	TF: DBD-Pfam\|HTH_1	Yes (cluster 2)
-	154595 - 155317 (+)	TC: gnl\|TC-DB\|K3LPY5\|1.B.6.2.13	Yes (cluster 2)
-	155332 - 155682 (+)	other	Yes (cluster 2)
-	156639 - 159005 (-)	CAZyme: GH50	Yes (cluster 2)
-	159234 - 160241 (+)	other	Yes (cluster 2)
-	160310 - 161590 (-)	TC: gnl\|TC-DB\|B9TSM9\|2.A.56.1.6	Yes (cluster 2)
-	161603 - 162133 (-)	other	Yes (cluster 2)
-	162108 - 163154 (-)	TC: gnl\|TC-DB\|Q9KR64\|2.A.56.1.6	Yes (cluster 2)
-	163414 - 165177 (-)	other	Yes (cluster 2)
-	165226 - 166164 (-)	other	Yes (cluster 2)
-	166179 - 167060 (-)	CAZyme: GH16	Yes (cluster 2)
-	167244 - 168079 (+)	other	Yes (cluster 2)
-	168214 - 170514 (+)	other	Yes (cluster 2)
-	170678 - 170977 (+)	TF: DBD-Pfam\|HTH_3,DBD-SUPERFAMILY\|0038966	Yes (cluster 2)
-	171545 - 173827 (-)	other	Yes (cluster 2)
-	173868 - 176057 (-)	CAZyme: GH50	Yes (cluster 2)
-	176532 - 179147 (-)	other	Yes (cluster 2)
-	179323 - 180585 (-)	STP: STP\|Pyr_redox_2	Yes (cluster 2)
-	180597 - 180905 (-)	other	Yes (cluster 2)
-	180975 - 182132 (-)	other	Yes (cluster 2)
-	182235 - 183035 (-)	CAZyme: CE1	Yes (cluster 2)
-	183074 - 184192 (-)	other	Yes (cluster 2)
-	184575 - 186137 (-)	TC: gnl\|TC-DB\|P96169\|2.A.21.3.2	Yes (cluster 2)
-	186530 - 187387 (+)	TF: DBD-Pfam\|HTH_AraC,DBD-Pfam\|HTH_AraC,DBD-SUPERFAMILY\|0036286,DBD-SUPERFAMILY\|0035607	Yes (cluster 2)
-	187851 - 189296 (+)	CAZyme: GH16	Yes (cluster 2)
-	189330 - 190877 (+)	other	Yes (cluster 2)
-	190923 - 192383 (+)	other	Yes (cluster 2)
-	192425 - 194308 (+)	other	Yes (cluster 2)
-	194359 - 196341 (+)	CAZyme: GH127	Yes (cluster 2)
-	196471 - 197460 (+)	other	Yes (cluster 2)
-	197697 - 198215 (-)	other	Yes (cluster 2)
-	198479 - 198915 (-)	other	Yes (cluster 2)
-	198964 - 200436 (-)	other	Yes (cluster 2)
-	201063 - 202133 (-)	other	Yes (cluster 2)
-	202297 - 203280 (-)	CAZyme: GH16	Yes (cluster 2)
-	203467 - 205899 (-)	other	Yes (cluster 2)
-	205924 - 208275 (-)	other	Yes (cluster 2)
-	208395 - 210071 (-)	CAZyme: GH82	Yes (cluster 2)
-	210230 - 210946 (-)	other	Yes (cluster 2)
-	210967 - 211698 (-)	other	Yes (cluster 2)
-	211698 - 213089 (-)	CAZyme: GH16	Yes (cluster 2)
-	213120 - 214088 (-)	CAZyme: GH16	Yes (cluster 2)
-	214166 - 215926 (-)	other	Yes (cluster 2)
-	215998 - 219663 (-)	other	Yes (cluster 2)
-	220545 - 221630 (-)	other	Yes (cluster 2)
-	221627 - 223207 (-)	other	Yes (cluster 2)
-	223220 - 224506 (-)	other	Yes (cluster 2)
-	224582 - 226708 (-)	TC: gnl\|TC-DB\|Q9LAE4\|1.B.14.8.1	Yes (cluster 2)
-	226882 - 227382 (+)	TF: DBD-Pfam\|MarR,DBD-SUPERFAMILY\|0040517	Yes (cluster 2)
-	227403 - 227867 (-)	TF: DBD-Pfam\|HTH_11,DBD-SUPERFAMILY\|0044258	Yes (cluster 2)
-	228002 - 229075 (+)	other	Yes (cluster 2)
-	229075 - 229998 (+)	TF: DBD-Pfam\|Crp	Yes (cluster 2)
-	230608 - 231657 (-)	CAZyme: GH16	Yes (cluster 2)
-	232368 - 232934 (+)	CDS	No
-	232900 - 234402 (+)	CDS	No

PUL ID	PUL0460
PubMed	29774012, Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.
Title	A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus.
Author	Schultz-Johansen M, Bech PK, Hennessy RC, Glaring MA, Barbeyron T, Czjzek M, Stougaard P
Abstract	Marine microbes are a rich source of enzymes for the degradation of diverse polysaccharides. Paraglaciecola hydrolytica S66(T) is a marine bacterium capable of hydrolyzing polysaccharides found in the cell wall of red macroalgae. In this study, we applied an approach combining genomic mining with functional analysis to uncover the potential of this bacterium to produce enzymes for the hydrolysis of complex marine polysaccharides. A special feature of P. hydrolytica S66(T) is the presence of a large genomic region harboring an array of carbohydrate-active enzymes (CAZymes) notably agarases and carrageenases. Based on a first functional characterization combined with a comparative sequence analysis, we confirmed the enzymatic activities of several enzymes required for red algal polysaccharide degradation by the bacterium. In particular, we report for the first time, the discovery of novel enzyme activities targeting furcellaran, a hybrid carrageenan containing both beta-carrageenan and kappa/beta-carrageenan motifs. Some of these enzymes represent a new subfamily within the CAZy classification. From the combined analyses, we propose models for the complete degradation of agar and kappa/beta-type carrageenan by P. hydrolytica S66(T). The novel enzymes described here may find value in new bio-based industries and advance our understanding of the mechanisms responsible for recycling of red algal polysaccharides in marine ecosystems.

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Degradation or Biosynthesis

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