Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0225 RT-PCR porphyran Bacteroides plebeius 23150581
Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19786-91. doi: 10.1073/pnas.1211002109. Epub 2012 Nov 12.
2012 Nov 27 degradation 36 12 GH16, GH117, GH2, GH2, GH50, GH154, GH105, GH16, GH86, GH86, GH29, GH16, GH2
PUL0393 enzyme activity assay, analysis of reaction products galactan Microbulbifer thermotolerans 20686828
Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94T, and insight into the unusual catalytic mechanism. Mar Biotechnol (NY). 2011 Jun;13(3):411-22. doi: 10.1007/s10126-010-9312-0. Epub 2010 Aug 5.
2011 Jun degradation 10 1 GH86, CBM6, GH16, CBM6
PUL0459 RNA-Seq, analysis of reaction products, enzyme activity assay agar Colwellia echini 31915221
A Multifunctional Polysaccharide Utilization Gene Cluster in Colwellia echini Encodes Enzymes for the Complete Degradation of kappa-Carrageenan, iota-Carrageenan, and Hybrid beta/kappa-Carrageenan. mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19.
2020 Jan 8 degradation 48 10 GH2, GH96, CBM6, GH96, CBM6, GH50, GH50, GH29, GH50, GH86, GH117
PUL0460 recombinant protein expression, RT-PCR, enzyme activity assay carrageenan, agar Paraglaciecola hydrolytica 29774012
A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus. Front Microbiol. 2018 May 3;9:839. doi: 10.3389/fmicb.2018.00839. eCollection 2018.
2018 degradation 108 18 GH2, GH29, GH63, GH86, CBM6, GH50, GH86, GH50, GH117, GH50, GH16, GH50, CE1, GH16, GH127, GH16, GH82, GH16, GH16, GH16
PUL0651 enzyme activity assay, NMR agar Gilvimarinus chinensis DSM 19667 33691998
Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19.
2021 Aug 1 degradation 63 14 CBM6, GH16_16, GH16, GH50, CBM6, CBM13, CBM6, GH16_16, GH16, GH50, GH117, GH16_3, GH16, GH2, GH50, GH127, GH167, CBM2, CBM6, GH86,, GH50,, GH86
PUL0652 RNA-Seq, enzyme activity assay, thin layer chromatography, liquid chromatography, mass spectrometry agar Colwellia echini A3 33811026
A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3(T). Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21. Epub 2021 May 26.
2021 May 26 degradation 54 10 CBM35, GH2, CBM6, GH96, CBM6, GH96, GH50, GH50, GH50, GH29, CBM13, GH50, GH86, GH117