Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0187

PubMed

26746717, Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.

Characterization method

qRT-PCR,enzyme activity assay

Genomic accession number

NC_012914.1

Nucelotide position range

1124076-1136568

Substrate

beta-glucan

Loci

PJDR2_RS04870-PJDR2_RS4900

Species

Paenibacillus sp. JDR-2/324057

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- PJDR2_RS04870 WP_015842573.1 0 - 1629 (-) NC_012914.1:1124076-1125705 -
- PJDR2_RS04875 WP_015842574.1 1639 - 3352 (-) NC_012914.1:1125715-1127428 -
- PJDR2_RS04880 WP_150106437.1 3487 - 4456 (+) NC_012914.1:1127563-1128532 -
- PJDR2_RS04885 WP_015842576.1 4469 - 5348 (+) NC_012914.1:1128545-1129424 -
- PJDR2_RS04890 WP_015842577.1 5411 - 9713 (+) NC_012914.1:1129487-1133789 -
- PJDR2_RS04895 WP_015842578.1 9806 - 10520 (+) NC_012914.1:1133882-1134596 -
- PJDR2_RS04900 WP_015842579.1 10768 - 12493 (+) NC_012914.1:1134844-1136569 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1629 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 No
- 1640 - 3352 (-) TC: gnl|TC-DB|F4LXP4|8.A.59.2.1 Yes
- 3488 - 4456 (+) TC: gnl|TC-DB|Q09LY7|3.A.1.1.9 Yes
- 4470 - 5348 (+) TC: gnl|TC-DB|Q09LY6|3.A.1.1.9 Yes
- 5412 - 9713 (+) CAZyme: CBM4|CBM54|CBM6|GH16 Yes
- 9807 - 10520 (+) CAZyme: GH16 Yes
- 10769 - 12493 (+) TC: gnl|TC-DB|C9RT46|3.A.1.1.9 Yes

PUL ID

PUL0187

PubMed

26746717, Appl Environ Microbiol. 2016 Jan 8;82(6):1789-1798. doi: 10.1128/AEM.03526-15.

Title

A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2.

Author

Chow V, Kim YS, Rhee MS, Sawhney N, St John FJ, Nong G, Rice JD, Preston JF

Abstract

Paenibacillus sp. strain JDR-2 (Paenibacillus JDR-2) secretes a multimodular cell-associated glycoside hydrolase family 10 (GH10) endoxylanase (XynA10A1) that catalyzes the depolymerization of methylglucuronoxylan (MeGXn) and rapidly assimilates the products of depolymerization. Efficient utilization of MeGXn has been postulated to result from the coupling of the processes of exocellular depolymerization and assimilation of oligosaccharide products, followed by intracellular metabolism. Growth and substrate utilization patterns with barley glucan and laminarin similar to those observed with MeGXn as a substrate suggest similar processes for 1,3-1,4-beta-glucan and 1,3-beta-glucan depolymerization and product assimilation. The Paenibacillus JDR-2 genome includes a cluster of genes encoding a secreted multimodular GH16 beta-glucanase (Bgl16A1) containing surface layer homology (SLH) domains, a secreted GH16 beta-glucanase with only a catalytic domain (Bgl16A2), transporter proteins, and transcriptional regulators. Recombinant Bgl16A1 and Bgl16A2 catalyze the formation of trisaccharides, tetrasaccharides, and larger oligosaccharides from barley glucan and of mono-, di-, tri-, and tetrasaccharides and larger oligosaccharides from laminarin. The lack of accumulation of depolymerization products during growth and a marked preference for polymeric glucan over depolymerization products support a process coupling extracellular depolymerization, assimilation, and intracellular metabolism for beta-glucans similar to that ascribed to the GH10/GH67 xylan utilization system in Paenibacillus JDR-2. Coordinate expression of genes encoding GH16 beta-glucanases, transporters, and transcriptional regulators supports their role as a regulon for the utilization of soluble beta-glucans. As in the case of the xylan utilization regulons, this soluble beta-glucan regulon provides advantages in the growth rate and yields on polymeric substrates and may be exploited for the efficient conversion of plant-derived polysaccharides to targeted products.