Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0312

PubMed

19139238, Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9.

Characterization method

RT-PCR

Genomic accession number

L41732.5

Nucelotide position range

1072-4481

Substrate

sucrose

Loci

lsdAB

Species

Gluconacetobacter diazotrophicus/33996

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

lsdA - AAB36606.1 0 - 1797 (+) L41732.5:1072-2869 2.4.1.10
lsdB - AAF16405.1 1805 - 3410 (+) L41732.5:2877-4482 3.2.1.65

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1755 (+) CAZyme: GH68 No
- 1806 - 3410 (+) CAZyme: GH32 No

PUL ID

PUL0312

PubMed

19139238, Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9.

Title

Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus.

Author

Menendez C, Banguela A, Caballero-Mellado J, Hernandez L

Abstract

Gluconacetobacter diazotrophicus utilizes plant sucrose with a constitutively expressed levansucrase (LsdA), producing extracellular levan, which may be degraded under energetically unfavored conditions. Reverse transcriptase-PCR analysis revealed that lsdA and the downstream exolevanase gene (lsdB) form an operon. lsdB transcription was induced during growth with low fructose concentrations (0.44 to 33 mM) and repressed by glucose. Transport of LsdB to the periplasm involved N-terminal signal peptide cleavage. Type II secretion mutants failed to transfer LsdB across the outer membrane, impeding levan hydrolysis.