dbCAN-PUL

PUL ID	PUL0312
PubMed	19139238, Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9.
Characterization method	RT-PCR
Genomic accession number	L41732.5
Nucelotide position range	1072-4481
Substrate	sucrose
Loci	lsdAB
Species	Gluconacetobacter diazotrophicus/33996
Degradation or Biosynthesis	degradation

PUL ID	PUL0312
PubMed	19139238, Appl Environ Microbiol. 2009 Mar;75(6):1782-5. doi: 10.1128/AEM.01887-08. Epub 2009 Jan 9.
Title	Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus.
Author	Menendez C, Banguela A, Caballero-Mellado J, Hernandez L
Abstract	Gluconacetobacter diazotrophicus utilizes plant sucrose with a constitutively expressed levansucrase (LsdA), producing extracellular levan, which may be degraded under energetically unfavored conditions. Reverse transcriptase-PCR analysis revealed that lsdA and the downstream exolevanase gene (lsdB) form an operon. lsdB transcription was induced during growth with low fructose concentrations (0.44 to 33 mM) and repressed by glucose. Transport of LsdB to the periplasm involved N-terminal signal peptide cleavage. Type II secretion mutants failed to transfer LsdB across the outer membrane, impeding levan hydrolysis.