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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0604

PubMed

31703861, Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.

Characterization method

microarray,qRT-PCR,culureing methods

Genomic accession number

NC_004567.2

Nucelotide position range

164479-171853

Substrate

galactomannooligosaccharide

Loci

LP_RS00750-LP_RS00770

Species

Lactobacillus plantarum WCFS1/1590

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- LP_RS00750 WP_003641780.1 0 - 867 (-) NC_004567.2:164479-165346 -
- LP_RS00755 WP_003641781.1 1000 - 2956 (-) NC_004567.2:165479-167435 -
- LP_RS00760 WP_011100912.1 3211 - 4717 (+) NC_004567.2:167690-169196 -
- LP_RS00765 WP_003641783.1 4697 - 5678 (+) NC_004567.2:169176-170157 -
- LP_RS00770 WP_003641784.1 5698 - 7375 (+) NC_004567.2:170177-171854 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 867 (-) CDS No
- 1001 - 2956 (-) TC: gnl|TC-DB|Q8NMD6|4.A.1.2.12 Yes
- 3212 - 4717 (+) CAZyme: GH32 Yes
- 4698 - 5678 (+) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 Yes
- 5699 - 7375 (+) CAZyme: GH13_31|GH13 Yes

PUL ID

PUL0604

PubMed

31703861, Food Microbiol. 2020 Apr;86:103336. doi: 10.1016/j.fm.2019.103336. Epub 2019 Sep 14.

Title

Transcriptional analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.

Author

Panwar D, Kapoor M

Abstract

Plant derived galactomannooligosaccharides (GMOS) are an emerging class of prebiotics, but no information is available on their utilization in lactobacilli at the molecular level. The current study aimed at identifying the genetic loci involved in the transport and catabolism of locust bean gum derived GMOS in Lactobacillus plantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degree of polymerization; DP carbohydrate source. Two genetic loci involved in cellobiose (~3.2 kb) and oligo-sucrose (~7.3 kb) utilization in L. plantarum WCFS1 were highly up-regulated up to 8.3 and up to 6.7-fold, respectively by GMOS utilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptome and qRT-PCR studies of L. plantarum WCFS1 suggested that un-substituted mannobiose (DP(2)) might be metabolized by proteins encoded by the cellobiose operon while, substituted DP(2) (galactomannose) and DP(3) (galactomannobiose) were most likely transported and catabolized by the oligo-sucrose utilization loci encoded proteins.