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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0385

PubMed

20013800, Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311.

Characterization method

ion trap liquid chromatography,mass spectrometry,target decoy database analysis,high performance anion exchange chromatography

Genomic accession number

NC_011898.1

Nucelotide position range

838275-864108

Substrate

cellulose

Loci

CCEL_RS03695-CCEL_RS03750

Species

Ruminiclostridium cellulolyticum/1521

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- CCEL_RS03695 WP_014313401.1 0 - 3924 (+) NC_011898.1:838275-842199 -
- CCEL_RS18230 WP_020814339.1 3936 - 4641 (+) NC_011898.1:842211-842916 -
- CCEL_RS03700 WP_015924275.1 4757 - 6926 (+) NC_011898.1:843032-845201 -
- CCEL_RS03705 WP_015924276.1 7054 - 8437 (+) NC_011898.1:845329-846712 -
- CCEL_RS03710 WP_015924277.1 8521 - 10699 (+) NC_011898.1:846796-848974 -
- CCEL_RS03715 WP_015924278.1 10791 - 13449 (+) NC_011898.1:849066-851724 -
- CCEL_RS03720 WP_015924279.1 13617 - 14310 (+) NC_011898.1:851892-852585 -
- CCEL_RS03725 WP_015924280.1 14336 - 16550 (+) NC_011898.1:852611-854825 -
- CCEL_RS03730 WP_015924281.1 16737 - 19011 (+) NC_011898.1:855012-857286 -
- CCEL_RS03735 WP_015924282.1 19048 - 20323 (+) NC_011898.1:857323-858598 -
- CCEL_RS03740 WP_015924283.1 20412 - 21993 (+) NC_011898.1:858687-860268 -
- CCEL_RS18235 WP_157668438.1 22009 - 22126 (+) NC_011898.1:860284-860401 -
- CCEL_RS03745 WP_049756855.1 22177 - 24190 (+) NC_011898.1:860452-862465 -
- CCEL_RS03750 WP_015924285.1 24229 - 25834 (+) NC_011898.1:862504-864109 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 4758 - 6926 (+) CAZyme: GH48 No
- 7055 - 8437 (+) CAZyme: GH8 No
- 8522 - 10699 (+) CAZyme: GH9|CBM3 No
- 10792 - 13449 (+) CAZyme: CBM4|GH9|CBM30 No
- 13618 - 14310 (+) CDS No
- 14337 - 16550 (+) CAZyme: GH9|CBM3 No
- 16738 - 19011 (+) CAZyme: GH9|CBM3 No
- 19049 - 20323 (+) CAZyme: GH5_17 No
- 20413 - 21993 (+) CAZyme: GH9 No
- 22010 - 22126 (+) CDS No
- 22178 - 24190 (+) CAZyme: PL11_1|PL11 No
- 24230 - 25834 (+) CAZyme: GH5_1 No

PUL ID

PUL0385

PubMed

20013800, Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311.

Title

Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses.

Author

Blouzard JC, Coutinho PM, Fierobe HP, Henrissat B, Lignon S, Tardif C, Pages S, de Philip P

Abstract

Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities were predicted. Among them, 62 dockerin-containing proteins bear catalytic modules from numerous carbohydrate-active enzymes' families and whose diversity reflects the chemical and structural complexity of the plant carbohydrate. The composition of the cellulosomes produced by C. cellulolyticum upon growth on different substrates (cellulose, xylan, and wheat straw) was investigated by LC MS/MS. The majority of the proteins encoded by the cip-cel operon, essential for cellulose degradation, were detected in all cellulosome preparations. In the presence of wheat straw, the natural and most complex of the substrates studied, additional proteins predicted to be involved in hemicellulose degradation were produced. A 32-kb gene cluster encodes the majority of these proteins, all harbouring carbohydrate-binding module 6 or carbohydrate-binding module 22 xylan-binding modules along dockerins. This newly identified xyl-doc gene cluster, specialised in hemicellulose degradation, comes in addition of the cip-cel operon for plant cell wall degradation. Hydrolysis efficiencies determined on the different substrates corroborates the finding that cellulosome composition is adapted to the growth substrate.