| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0040 | Northern Blot, enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 12896991, 1398087, 11844767 A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome. J Bacteriol. 2003 Aug;185(16):4727-33. doi: 10.1128/JB.185.16.4727-4733.2003. Gene. 1992 Sep 21;119(1):17-28. doi: 10.1016/0378-1119(92)90062-t. J Bacteriol. 2002 Mar;184(5):1378-84. doi: 10.1128/JB.184.5.1378-1384.2002. |
2003 Aug,1992 Sep 21,2002 Mar | degradation | 6 | 6 | GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1, GH5 |
| PUL0083 | label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay | lignocellulose | Ruminiclostridium papyrosolvens | 31338125 Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019. |
2019 | degradation | 12 | 10 | cohesin, CBM3, GH48, GH8, CBM3, GH9, CBM4, CBM30, GH9, CBM3, GH9, CBM3, GH9, GH5_17, GH9, GH5_7, GH5_1 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 12 | 10 | GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1 |
| PUL0434 | SDS-PAGE, Western Blot | mannooligosaccharide | Clostridium cellulovorans | 10613891 The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA. J Bacteriol. 2000 Jan;182(1):244-7. doi: 10.1128/JB.182.1.244-247.2000. |
2000 Jan | degradation | 8 | 2 | CBM30, CBM4, GH9, GH9, GH5_17, CBM4, GH9, CBM4 |
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