Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0040 Northern Blot, enzyme activity assay cellulose Ruminiclostridium cellulolyticum 12896991, 1398087, 11844767
A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome. J Bacteriol. 2003 Aug;185(16):4727-33. doi: 10.1128/jb.185.16.4727-4733.2003. Gene. 1992 Sep 21;119(1):17-28. doi: 10.1016/0378-1119(92)90062-t. J Bacteriol. 2002 Mar;184(5):1378-84. doi: 10.1128/jb.184.5.1378-1384.2002.
2003 Aug,1992 Sep 21,2002 Mar degradation 6 6 GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1, GH5
PUL0086 label-free quantitative proteomics, functional enrichment analysis, enzyme activity assay lignocellulose Ruminiclostridium papyrosolvens 31338125
Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation. Biotechnol Biofuels. 2019 Jul 15;12:183. doi: 10.1186/s13068-019-1522-8. eCollection 2019.
2019 degradation 3 3 PL10, PL10_1, CE8, CE12, PL11_1, PL11
PUL0192 RNA-Seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 27 12 GH106, GH28, GH28, CE12, GH2, GH43_18, GH43_34, GH43, CBM32, GH42, GH28, PL11, PL11_1, PL26, CE12, GH105, PL11_1, GH105
PUL0193 RNA-Seq, RT-PCR, qPCR pectin Bacteroides xylanisolvens 26920945
Unraveling the pectinolytic function of Bacteroides xylanisolvens using a RNA-seq approach and mutagenesis. BMC Genomics. 2016 Feb 27;17:147. doi: 10.1186/s12864-016-2472-1.
2016 Feb 27 degradation 30 10 GH2, PL11_1, PL11, GH28, GH117, PL11_1, PL11, GH28, GH105, GH28
PUL0249 microarray rhamnogalacturonan Bacillus subtilis 17449691
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. Appl Environ Microbiol. 2007 Jun;73(12):3803-13. doi: 10.1128/AEM.00147-07. Epub 2007 Apr 20.
2007 Jun degradation 12 6 GH105, CE12, PL11_1, CE12, PL11, PL11_1, PL11, CE12, GH42
PUL0385 ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography cellulose Ruminiclostridium cellulolyticum 20013800
Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311.
2010 Feb degradation 12 10 GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1
PUL0564 microarray, qPCR rhamnogalacturonan Bacteroides thetaiotaomicron 18996345, 22205877
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2008 Nov 13,2011 Dec degradation 39 23 GH106, GH28, GH28, CE12, GH2, GH43, GH43_18, GH42, GH28, CE4, CE0, GH28, GH2, GH27, CBM35, GH35, PL9, PL9_1, PL26, CE12, GH105, PL11, PL11_1, GH105, CE4, CE0, CE6, GH2, PL9_1