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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0622

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKE010000021.1

Nucelotide position range

12378-52329

Substrate

xylan

Loci

HPS55_11495-HPS55_11570

Species

Prevotella sp. PROD/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS55_11495 NPE14934.1 0 - 1419 (-) JABKKE010000021.1:12378-13797 -
- HPS55_11500 NPE14935.1 1527 - 3105 (-) JABKKE010000021.1:13905-15483 -
- HPS55_11505 NPE14936.1 3594 - 6867 (-) JABKKE010000021.1:15972-19245 -
- HPS55_11510 NPE14937.1 6869 - 8612 (-) JABKKE010000021.1:19247-20990 -
- HPS55_11515 NPE14938.1 8642 - 10769 (-) JABKKE010000021.1:21020-23147 -
- HPS55_11520 NPE14939.1 10790 - 12533 (-) JABKKE010000021.1:23168-24911 -
- HPS55_11525 NPE14940.1 12568 - 15961 (-) JABKKE010000021.1:24946-28339 -
- HPS55_11530 NPE14941.1 15973 - 18892 (-) JABKKE010000021.1:28351-31270 -
- HPS55_11535 NPE14942.1 19178 - 19796 (-) JABKKE010000021.1:31556-32174 -
- HPS55_11540 NPE14943.1 19882 - 22498 (-) JABKKE010000021.1:32260-34876 -
- HPS55_11545 NPE14944.1 22720 - 27238 (-) JABKKE010000021.1:35098-39616 -
- HPS55_11550 NPE14945.1 27774 - 30366 (+) JABKKE010000021.1:40152-42744 -
- HPS55_11555 - 30368 - 31691 (+) JABKKE010000021.1:42746-44069 -
- HPS55_11560 NPE14946.1 32393 - 33836 (+) JABKKE010000021.1:44771-46214 -
- HPS55_11565 NPE14947.1 34117 - 36730 (+) JABKKE010000021.1:46495-49108 -
- HPS55_11570 NPE14948.1 36973 - 39952 (+) JABKKE010000021.1:49351-52330 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1419 (-) CDS No
- 1528 - 3105 (-) CDS No
- 3595 - 6867 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 6870 - 8612 (-) other Yes
- 8643 - 10769 (-) other Yes
- 10791 - 12533 (-) other Yes
- 12569 - 15961 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 15974 - 18892 (-) other Yes
- 19179 - 19796 (-) other Yes
- 19883 - 22498 (-) other Yes
- 22721 - 27238 (-) CAZyme: PL11|PL11_1 Yes
- 27775 - 30366 (+) CAZyme: GH3| GH3 Yes
- 32394 - 33836 (+) CAZyme: CBM13| GH43_7|GH43_7 Yes
- 34118 - 36730 (+) CAZyme: PL11_1|PL11_1 Yes
- 36974 - 39952 (+) CAZyme: GH2|GH2 Yes

PUL ID

PUL0622

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.