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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0573

PubMed

19648249, J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31.

Characterization method

enzyme activity assay,electrophoretic mobility shift assay,RT-PCR,qRT-PCR

Genomic accession number

NC_010572.1

Nucelotide position range

5564640-5570805

Substrate

cellobiose,cellulose,cellooligosaccharide

Loci

SGR_RS23570-SGR_RS23590

Species

Streptomyces griseus/1911

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SGR_RS23570 WP_012380827.1 0 - 1323 (+) NC_010572.1:5564640-5565963 -
- SGR_RS23575 WP_012380828.1 1491 - 2490 (+) NC_010572.1:5566131-5567130 -
- SGR_RS23580 WP_012380829.1 2504 - 3425 (+) NC_010572.1:5567144-5568065 -
- SGR_RS23585 WP_012380830.1 3491 - 4949 (+) NC_010572.1:5568131-5569589 3.2.1.23
- SGR_RS23590 WP_003968984.1 5128 - 6166 (+) NC_010572.1:5569768-5570806 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1323 (+) TC: gnl|TC-DB|Q9X9R7|3.A.1.1.23 Yes
- 1492 - 2490 (+) TC: gnl|TC-DB|Q9X9R6|3.A.1.1.23 Yes
- 2505 - 3425 (+) TC: gnl|TC-DB|Q9X9R5|3.A.1.1.23 Yes
- 3492 - 4949 (+) CAZyme: GH1 Yes
- 5129 - 6166 (+) STP: STP|LacI,STP|Peripla_BP_3 No

PUL ID

PUL0573

PubMed

19648249, J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31.

Title

CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus.

Author

Marushima K, Ohnishi Y, Horinouchi S

Abstract

Streptomyces griseus mutants exhibiting deficient glucose repression of beta-galactosidase activity on lactose-containing minimal medium supplemented with a high concentration of glucose were isolated. One of these mutants had a 12-bp deletion in cebR, which encodes a LacI/GalR family regulator. Disruption of cebR in the wild-type strain caused the same phenotype as the mutant, indicating that CebR is required for glucose repression of beta-galactosidase activity. Recombinant CebR protein bound to a 14-bp inverted-repeat sequence (designated the CebR box) present in the promoter regions of cebR and the putative cellobiose utilization operon, cebEFG-bglC. The DNA-binding activity of CebR was impaired by cellooligosaccharides, including cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose. In agreement with this observation, transcription from the cebE and cebR promoters was greatly enhanced by the addition of cellobiose to the medium. Seven other genes containing one or two CebR boxes in their upstream regions were found in the S. griseus genome. Five of these genes encode putative secreted proteins: two cellulases, a cellulose-binding protein, a pectate lyase, and a protein of unknown function. These five genes and cebEFG-bglC were transcribed at levels 4 to 130 times higher in the DeltacebR mutant than in the wild-type strain, as determined by quantitative reverse transcription-PCR. These findings indicate that CebR is a master regulator of cellulose/cellooligosaccharide catabolism. Unexpectedly, the DeltacebR mutant formed very few aerial hyphae on lactose-containing medium, demonstrating a link between carbon source utilization and morphological development.