| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0004 | enzyme activity assay, substrate binding assay | glucose, cellobiose, maltose | uncultured bacterium | 26827771 A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol. 2016 Mar;84:24-31. doi: 10.1016/j.enzmictec.2015.12.005. Epub 2015 Dec 15. |
2016 Mar | degradation | 2 | 1 | GH1 |
| PUL0011 | enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 8936327 Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Microbiology (Reading). 1996 Apr;142 ( Pt 4):1013-1023. doi: 10.1099/00221287-142-4-1013. |
1996 Apr | degradation | 4 | 4 | GH48, GH8, CBM3, GH9, CBM4, GH9 |
| PUL0015 | microarray | cellobiose | Lactococcus lactis | 28970222 Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23):e01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1. |
2017 Dec 1 | degradation | 4 | 1 | GH9 |
| PUL0016 | microarray | cellobiose | Lactococcus lactis | 28970222 Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363. Appl Environ Microbiol. 2017 Nov 16;83(23):e01279-17. doi: 10.1128/AEM.01279-17. Print 2017 Dec 1. |
2017 Dec 1 | degradation | 5 | 1 | GH1 |
| PUL0017 | qRT-PCR, Western Blot, isothermal titration calorimetry | cellobiose | Ruminiclostridium cellulolyticum | 29093754 A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis. Biotechnol Biofuels. 2017 Oct 30;10:250. doi: 10.1186/s13068-017-0933-7. eCollection 2017. |
2017 | degradation | 9 | 1 | GH94 |
| PUL0019 | enzyme activity assay, Northern Blot | lichenan, cellobiose, beta-glucoside | Bacillus subtilis | 8990303 Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis. J Bacteriol. 1997 Jan;179(2):496-506. doi: 10.1128/jb.179.2.496-506.1997. |
1997 Jan | degradation | 6 | 1 | GH4 |
| PUL0022 | RT-PCR, gene deletion mutant and growth assay, enzyme activity assay | cellobiose | Bacillus coagulans | 30519284 Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018. |
2018 | degradation | 6 | 1 | GH1 |
| PUL0023 | RT-PCR, gene deletion mutant and growth assay, enzyme activity assay | cellobiose | Bacillus coagulans | 30519284 Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons. Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018. |
2018 | degradation | 5 | 1 | GH1 |
| PUL0040 | Northern Blot, enzyme activity assay | cellulose | Ruminiclostridium cellulolyticum | 12896991, 1398087, 11844767 A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Cel9M, a new family 9 cellulase of the Clostridium cellulolyticum cellulosome. J Bacteriol. 2003 Aug;185(16):4727-33. doi: 10.1128/JB.185.16.4727-4733.2003. Gene. 1992 Sep 21;119(1):17-28. doi: 10.1016/0378-1119(92)90062-t. J Bacteriol. 2002 Mar;184(5):1378-84. doi: 10.1128/JB.184.5.1378-1384.2002. |
2003 Aug,1992 Sep 21,2002 Mar | degradation | 6 | 6 | GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1, GH5 |
| PUL0041 | Southern Blot, enzyme activity assay | cellobiose | Klebsiella oxytoca | 9023916 Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl Environ Microbiol. 1997 Feb;63(2):355-63. doi: 10.1128/aem.63.2.355-363.1997. |
1997 Feb | degradation | 3 | 1 | GH1 |
| PUL0050 | fosmid library screen | cellulose, galactomannan | feces metagenome | 29601586 Two new gene clusters involved in the degradation of plant cell wall from the fecal microbiota of Tunisian dromedary. PLoS One. 2018 Mar 30;13(3):e0194621. doi: 10.1371/journal.pone.0194621. eCollection 2018. |
2018 | degradation | 20 | 7 | GH5_4, GH5, GH26, GH130, GH3, GH94 |
| PUL0186 | gene deletion mutant and growth assay | cellobiose | Streptococcus pneumoniae | 17028271 The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6. J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6. |
2007 Feb | degradation | 7 | 1 | GH1 |
| PUL0195 | RT-PCR | cellobiose | Clostridium acetobutylicum | 26691835 PTS regulation domain-containing transcriptional activator CelR and sigma factor sigma(54) control cellobiose utilization in Clostridium acetobutylicum. Mol Microbiol. 2016 Apr;100(2):289-302. doi: 10.1111/mmi.13316. Epub 2016 Feb 9. |
2016 Apr | degradation | 5 | 1 | GH1 |
| PUL0221 | fosmid library screen | cellulose | uncultured bacterium Contig1529 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 10 | 5 | GH35, GH105, GH3, GH5_4 |
| PUL0222 | fosmid library screen | cellulose | uncultured bacterium Contig196 | 24223817 Metagenomic insights into the carbohydrate-active enzymes carried by the microorganisms adhering to solid digesta in the rumen of cows. PLoS One. 2013 Nov 5;8(11):e78507. doi: 10.1371/journal.pone.0078507. eCollection 2013. |
2013 | degradation | 7 | 3 | GH5, GH5_7, GH5_4, GH26 |
| PUL0224 | RT-PCR, qRT-PCR | cellulose | Ruminiclostridium cellulolyticum | 23418511 A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013;8(2):e56063. doi: 10.1371/journal.pone.0056063. Epub 2013 Feb 13. |
2013 | degradation | 16 | 14 | GH43_16, GH43, CBM6, GH10, CBM6, GH43, GH43_29, CBM6, CBM6, CE1, GH43_10, CBM6, GH62, CBM6, GH43, GH43_29, CBM6, GH146, CBM22, GH27, CBM6, GH59, CBM6, GH2, CBM6, GH62, CBM6, CE6, CBM32, GH95, CBM6, GH30_8, CBM6 |
| PUL0239 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00026 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 18 | 10 | GH26, GH5_4, GH5_7, GH130, GH26, CE7, GH36, GH3 |
| PUL0240 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00028 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 11 | 5 | GH31, GH9, GH26 |
| PUL0241 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00033 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 7 | 3 | GH5, GH5_4, GH36 |
| PUL0242 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00044 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 9 | 3 | GH31, CBM72, GH5_4, GH26 |
| PUL0243 | fosmid library screen, sequence homology analysis | cellulose | Prevotella sp. Sc00066 | 24448980 Analysis of the bovine rumen microbiome reveals a diversity of Sus-like polysaccharide utilization loci from the bacterial phylum Bacteroidetes. J Ind Microbiol Biotechnol. 2014 Mar;41(3):601-6. doi: 10.1007/s10295-013-1395-y. Epub 2014 Jan 22. |
2014 Mar | degradation | 12 | 4 | GH5_38, GH94, GH36 |
| PUL0311 | enzyme activity assay | cellulose | Escherichia coli | 31455320 Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4. BMC Biotechnol. 2019 Aug 27;19(1):63. doi: 10.1186/s12896-019-0556-0. |
2019 Aug 27 | degradation | 4 | 2 | GT2, GH8 |
| PUL0331 | carbon utilization assay | cellobiose | Aliivibrio fischeri | 18487409 Identification of a cellobiose utilization gene cluster with cryptic beta-galactosidase activity in Vibrio fischeri. Appl Environ Microbiol. 2008 Jul;74(13):4059-69. doi: 10.1128/AEM.00190-08. Epub 2008 May 16. |
2008 Jul | degradation | 6 | 1 | GH1 |
| PUL0385 | ion trap liquid chromatography, mass spectrometry, target decoy database analysis, high performance anion exchange chromatography | cellulose | Ruminiclostridium cellulolyticum | 20013800 Modulation of cellulosome composition in Clostridium cellulolyticum: adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses. Proteomics. 2010 Feb;10(3):541-54. doi: 10.1002/pmic.200900311. |
2010 Feb | degradation | 12 | 10 | GH48, GH8, GH9, CBM3, CBM4, GH9, CBM30, GH9, CBM3, GH9, CBM3, GH5_17, GH9, PL11_1, PL11, GH5_1 |
| PUL0391 | microarray | cellulose, xylan, xyloglucan, pectin, mannan | Caldicellulosiruptor bescii | 21227922 Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725. Nucleic Acids Res. 2011 Apr;39(8):3240-54. doi: 10.1093/nar/gkq1281. Epub 2011 Jan 11. |
2011 Apr | degradation | 15 | 10 | PL11, CBM35, GH12, CBM3, CBM2, CE12, PL3_1, PL3, CBM66, PL9_1, CBM35, CBM66, PL9, CBM22, GH12, CBM0, CBM3, CBM2, CBM1, GH48, GH10, CBM9, GH44, CBM35, GH12, CBM44, CBM3, CBM2, CBM76, CBM10, GH5, GH5_8, CBM35, GH12, CBM5, CBM3, CBM2, GH74, CBM1, GH48, GT39, CBM3, GH9, GH5_8, CBM3, GH5_1, GH5_8, CBM3, GH48, GH9 |
| PUL0413 | enzyme activity assay, reducing-sugar assay | cellobiose | uncultured bacterium contig00059 | 30116044 Functional metagenomics reveals abundant polysaccharide-degrading gene clusters and cellobiose utilization pathways within gut microbiota of a wood-feeding higher termite. ISME J. 2019 Jan;13(1):104-117. doi: 10.1038/s41396-018-0255-1. Epub 2018 Aug 16. |
2019 Jan | degradation | 31 | 1 | GH44 |
| PUL0423 | clone and expression, enzyme activity assay | cellobiose | Thermotoga neapolitana | 10960102 Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana. J Bacteriol. 2000 Sep;182(18):5172-9. doi: 10.1128/JB.182.18.5172-5179.2000. |
2000 Sep | degradation | 3 | 2 | GH94 |
| PUL0475 | clone and expression, gene deletion mutant and growth assay | cellobiose, cellotriose | Streptomyces reticuli | 10347054 Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl Environ Microbiol. 1999 Jun;65(6):2636-43. doi: 10.1128/AEM.65.6.2636-2643.1999. |
1999 Jun | degradation | 7 | 1 | GH18, CBM2 |
| PUL0538 | RNA-Seq | galactomannan, glucomannan, cellobiose, xyloglucan | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 15 | 7 | GH130, GH26, GH26, GH5_2, GH5, GH5_7, CE7, GH3 |
| PUL0543 | RNA-Seq | cellobiose, beta-glucan, glucosamine | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 6 | 3 | GH30, GH30_3, GH2, CBM57, GH88 |
| PUL0546 | RNA-Seq | arabinogalactan, cellobiose | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 8 | 4 | GH2, GH30_4, GH51 |
| PUL0551 | RNA-Seq | cellobiose, beta-glucan, glucomannan, laminarin | Bacteroides cellulosilyticus | 23976882 Effects of diet on resource utilization by a model human gut microbiota containing Bacteroides cellulosilyticus WH2, a symbiont with an extensive glycobiome. PLoS Biol. 2013;11(8):e1001637. doi: 10.1371/journal.pbio.1001637. Epub 2013 Aug 20. |
2013 | degradation | 6 | 2 | GH16, GH3 |
| PUL0570 | clone and expression, enzyme activity assay | cellobiose | Corynebacterium glutamicum | 12777497 A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0. |
2003 Jun | degradation | 3 | 1 | GH1 |
| PUL0573 | enzyme activity assay, electrophoretic mobility shift assay, RT-PCR, qRT-PCR | cellobiose, cellulose, cellooligosaccharide | Streptomyces griseus | 19648249 CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus. J Bacteriol. 2009 Oct;191(19):5930-40. doi: 10.1128/JB.00703-09. Epub 2009 Jul 31. |
2009 Oct | degradation | 5 | 1 | GH1 |
| PUL0577 | SDS-PAGE, enzyme activity assay | chitin, chitobiose, cellobiose | Photobacterium profundum | 21098515 Elucidation of exo-beta-D-glucosaminidase activity of a family 9 glycoside hydrolase (PBPRA0520) from Photobacterium profundum SS9. Glycobiology. 2011 Apr;21(4):503-11. doi: 10.1093/glycob/cwq191. Epub 2010 Nov 22. |
2011 Apr | degradation | 11 | 3 | GH94, GH20, GH9 |
| PUL0583 | enzyme activity assay, gene deletion mutant and growth assay | cellobiose | Geobacillus stearothermophilus | 8407820 Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli. J Bacteriol. 1993 Oct;175(20):6441-50. doi: 10.1128/jb.175.20.6441-6450.1993. |
1993 Oct | degradation | 5 | 1 | CE0 |
| PUL0585 | microarray, gene deletion mutant and growth assay, beta-galactosidase assays | cellobiose | Streptococcus pneumoniae | 21778207 CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae. Microbiology (Reading). 2011 Oct;157(Pt 10):2854-2861. doi: 10.1099/mic.0.051359-0. Epub 2011 Jul 21. |
2011 Oct | degradation | 7 | 1 | GH1 |
| PUL0671 | gene deletion mutant and growth assay, enzyme activity assay, Western blot, qPCR | cellulose | Cytophaga hutchinsonii ATCC 33406 | 34731049 A Type IX Secretion System Substrate Involved in Crystalline Cellulose Degradation by Affecting Crucial Cellulose Binding Proteins in Cytophaga hutchinsonii. Appl Environ Microbiol. 2022 Jan 25;88(2):e0183721. doi: 10.1128/AEM.01837-21. Epub 2021 Nov 3. |
2022 Jan 25 | degradation | 6 | 0 | NA |
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