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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0331

PubMed

18487409, Appl Environ Microbiol. 2008 Jul;74(13):4059-69. doi: 10.1128/AEM.00190-08. Epub 2008 May 16.

Characterization method

carbon utilization assay

Genomic accession number

NC_006840.2

Nucelotide position range

662465-667978

Substrate

cellobiose

Loci

celCBGKAI

Species

Aliivibrio fischeri/668

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- VF_RS03140 WP_011261349.1 0 - 1356 (+) NC_006840.2:662465-663821 -
- VF_RS03145 WP_005417897.1 1388 - 1694 (+) NC_006840.2:663853-664159 -
- VF_RS03150 WP_005417898.1 1733 - 3143 (+) NC_006840.2:664198-665608 -
- VF_RS03155 WP_011261350.1 3169 - 4105 (+) NC_006840.2:665634-666570 -
- VF_RS03160 WP_011261351.1 4116 - 4440 (+) NC_006840.2:666581-666905 -
- VF_RS03165 WP_011261352.1 4509 - 5514 (+) NC_006840.2:666974-667979 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1356 (+) TC: gnl|TC-DB|Q72XQ0|4.A.3.2.8 Yes
- 1389 - 1694 (+) TC: gnl|TC-DB|Q9KSH5|4.A.3.2.6 Yes
- 1734 - 3143 (+) CAZyme: GH1 Yes
- 3170 - 4105 (+) other Yes
- 4117 - 4440 (+) TC: gnl|TC-DB|C4X746|4.A.3.2.7 Yes
- 4510 - 5514 (+) STP: STP|LacI,STP|Peripla_BP_3 No

PUL ID

PUL0331

PubMed

18487409, Appl Environ Microbiol. 2008 Jul;74(13):4059-69. doi: 10.1128/AEM.00190-08. Epub 2008 May 16.

Title

Identification of a cellobiose utilization gene cluster with cryptic beta-galactosidase activity in Vibrio fischeri.

Author

Adin DM, Visick KL, Stabb EV

Abstract

Cellobiose utilization is a variable trait that is often used to differentiate members of the family Vibrionaceae. We investigated how Vibrio fischeri ES114 utilizes cellobiose and found a cluster of genes required for growth on this beta-1,4-linked glucose disaccharide. This cluster includes genes annotated as a phosphotransferase system II (celA, celB, and celC), a glucokinase (celK), and a glucosidase (celG). Directly downstream of celCBGKA is celI, which encodes a LacI family regulator that represses cel transcription in the absence of cellobiose. When the celCBGKAI gene cluster was transferred to cellobiose-negative strains of Vibrio and Photobacterium, the cluster conferred the ability to utilize cellobiose. Genomic analyses of naturally cellobiose-positive Vibrio species revealed that V. salmonicida has a homolog of the celCBGKAI cluster, but V. vulnificus does not. Moreover, bioinformatic analyses revealed that CelG and CelK share the greatest homology with glucosidases and glucokinases in the phylum Firmicutes. These observations suggest that distinct genes for cellobiose utilization have been acquired by different lineages within the family Vibrionaceae. In addition, the loss of the celI regulator, but not the structural genes, attenuated the ability of V. fischeri to compete for colonization of its natural host, Euprymna scolopes, suggesting that repression of the cel gene cluster is important in this symbiosis. Finally, we show that the V. fischeri cellobioase (CelG) preferentially cleaves beta-d-glucose linkages but also cleaves beta-d-galactose-linked substrates such as 5-bromo-4-chloro-3-indolyl-beta-d-galactoside (X-gal), a finding that has important implications for the use of lacZ as a marker or reporter gene in V. fischeri.