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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0022

PubMed

30519284, Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.

Characterization method

RT-PCR,gene deletion mutant and growth assay,enzyme activity assay

Genomic accession number

NC_016023.1

Nucelotide position range

736768-743451

Substrate

cellobiose

Loci

BCOA_RS20690-BCOA_RS20715

Species

Bacillus coagulans/1398

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BCOA_RS20690 WP_014096055.1 0 - 1920 (-) NC_016023.1:736768-738688 -
- BCOA_RS20695 WP_014096056.1 2217 - 3654 (-) NC_016023.1:738985-740422 -
celB BCOA_RS20700 WP_014096057.1 3815 - 5171 (-) NC_016023.1:740583-741939 -
- BCOA_RS20705 WP_014096058.1 5192 - 5696 (-) NC_016023.1:741960-742464 -
- BCOA_RS20710 WP_014096059.1 5894 - 6218 (-) NC_016023.1:742662-742986 -
- BCOA_RS20715 WP_041817903.1 6351 - 6684 (-) NC_016023.1:743119-743452 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1920 (-) STP: STP|HTH_11,STP|Mga No
- 2218 - 3654 (-) CAZyme: GH1 Yes
celB 3816 - 5171 (-) TC: gnl|TC-DB|Q72XQ0|4.A.3.2.8 Yes
- 5193 - 5696 (-) other Yes
- 5895 - 6218 (-) other Yes
- 6352 - 6684 (-) TC: gnl|TC-DB|P46319|4.A.3.2.2 Yes

PUL ID

PUL0022

PubMed

30519284, Biotechnol Biofuels. 2018 Dec 1;11:320. doi: 10.1186/s13068-018-1323-5. eCollection 2018.

Title

Simultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons.

Author

Zheng Z, Jiang T, Zou L, Ouyang S, Zhou J, Lin X, He Q, Wang L, Yu B, Xu H, Ouyang J

Abstract

BACKGROUND: The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primarily glucose and xylose, is critical for the viability of lignocellulosic biorefineries. However, the phenomenon of glucose repression prevents co-utilization of both glucose and xylose in cellulosic hydrolysates. RESULTS: To circumvent glucose repression, co-utilization of cellobiose and xylose by Bacillus coagulans NL01 was investigated. During co-fermentation of cellobiose and xylose, B. coagulans NL01 simultaneously consumed the sugar mixtures and exhibited an improved lactic acid yield compared with co-fermentation of glucose and xylose. Moreover, the cellobiose metabolism of B. coagulans NL01 was investigated for the first time. Based on comparative genomic analysis, two gene clusters that encode two different operons of the cellobiose-specific phosphoenolpyruvate-dependent phosphotransferase system (assigned as CELO1 and CELO2) were identified. For CELO1, five genes were arranged as celA (encoding EIIA(cel)), celB (encoding EIIB(cel)), celC (encoding EIIC(cel)), pbgl (encoding 6-phospho-beta-glucosidase), and celR (encoding a transcriptional regulator), and these genes were found to be ubiquitous in different B. coagulans strains. Based on gene knockout results, CELO1 was confirmed to be responsible for the transport and assimilation of cellobiose. For CELO2, the five genes were arranged as celR, celB, celA, celX (encoding DUF871 domain-containing protein), and celC, and these genes were only found in some B. coagulans strains. However, through a comparison of cellobiose fermentation by NL01 and DSM1 that only possess CELO1, it was observed that CELO2 might also play an important role in the utilization of cellobiose in vivo despite the fact that no pbgl gene was found. When CELO1 or CELO2 was expressed in Escherichia coli, the recombinant strain exhibited distinct cellobiose uptake and consumption. CONCLUSIONS: This study demonstrated the cellobiose-assimilating pathway of B. coagulans and provided a new co-utilization strategy of cellobiose and xylose to overcome the obstacles that result from glucose repression in a biorefinery system.