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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0570

PubMed

12777497, Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0.

Characterization method

clone and expression,enzyme activity assay

Genomic accession number

AF508972

Nucelotide position range

1307-5555

Substrate

cellobiose

Loci

bglFAG

Species

Corynebacterium glutamicum/1718

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

bglF - AAO15901.1 196 - 2053 (+) AF508972.1:1503-3360 -
bglA - AAO15902.1 2113 - 3523 (+) AF508972.1:3420-4830 -
bglG - AAO15903.1 3562 - 4432 (+) AF508972.1:4869-5739 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 197 - 2053 (+) TC: gnl|TC-DB|Q8GGK3|4.A.1.2.5 Yes
- 2114 - 3523 (+) CAZyme: GH1 Yes
- 3563 - 4432 (+) CDS No

PUL ID

PUL0570

PubMed

12777497, Microbiology (Reading). 2003 Jun;149(Pt 6):1569-1580. doi: 10.1099/mic.0.26053-0.

Title

A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose.

Author

Kotrba P, Inui M, Yukawa H

Abstract

A catabolic system involved in the utilization of beta-glucosides in Corynebacterium glutamicum R and its spontaneous mutant variants allowing uptake of cellobiose were investigated. The system comprises a beta-glucoside-specific Enzyme IIBCA component (gene bglF) of the phosphotransferase system (PTS), a phospho-beta-glucosidase (bglA) and an antiterminator protein (bglG) from the BglG/SacY family of transcription regulators. The results suggest that transcription antitermination is involved in control of induction and carbon catabolite repression of bgl genes, which presumably form an operon. Functional analysis of the bglF and bglA products revealed that they are simultaneously required for uptake, phosphorylation and breakdown of methyl beta-glucoside, salicin and arbutin. Although cellobiose is not normally a substrate for BglF permease and is not utilized by C. glutamicum R, cellobiose-utilizing mutants can be obtained. The mutation responsible was mapped to the bgl locus and sequenced, and point mutations were found in codon 317 of bglF. These led to substitutions V317A and/or V317M near the putative PTS active-site H313 in the membrane-spanning IIC domain of BglF and allowed BglF to act on cellobiose. Such results strengthen the evidence that the IIC domains can be regarded as selectivity filters of the PTS.