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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0186

PubMed

17028271, J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6.

Characterization method

gene deletion mutant and growth assay

Genomic accession number

AE007317.1

Nucelotide position range

274499-281042

Substrate

cellobiose

Loci

spr0276-spr0282

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

bglA spr0276 AAK99080.1 0 - 1437 (+) AE007317.1:274499-275936 3.2.1.86
- spr0277 AAK99081.1 1488 - 1728 (+) AE007317.1:275987-276227 -
PTS-EII spr0278 AAK99082.1 1830 - 2172 (+) AE007317.1:276329-276671 2.7.1.69
bglG spr0279 AAK99083.1 2289 - 4269 (+) AE007317.1:276788-278768 -
PTS-EII spr0280 AAK99084.1 4278 - 4593 (+) AE007317.1:278777-279092 2.7.1.69
- spr0281 AAK99085.1 4611 - 5118 (+) AE007317.1:279110-279617 -
PTS-EII spr0282 AAK99086.1 5197 - 6544 (+) AE007317.1:279696-281043 2.7.1.69

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

bglA 1 - 1437 (+) CAZyme: GH1 Yes
- 1489 - 1728 (+) TC: gnl|TC-DB|C3WG44|2.A.123.2.3 Yes
PTS-EII 1831 - 2172 (+) other Yes
bglG 2290 - 4269 (+) STP: STP|HTH_11,STP|Mga Yes
PTS-EII 4279 - 4593 (+) TC: gnl|TC-DB|P46319|4.A.3.2.2 Yes
- 4612 - 5118 (+) other Yes
PTS-EII 5198 - 6544 (+) TC: gnl|TC-DB|Q72XQ0|4.A.3.2.8 Yes

PUL ID

PUL0186

PubMed

17028271, J Bacteriol. 2007 Feb;189(4):1342-50. doi: 10.1128/JB.01170-06. Epub 2006 Oct 6.

Title

The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6.

Author

McKessar SJ, Hakenbeck R

Abstract

The two-component system TCS08 is one of the regulatory systems that is important for virulence of Streptococcus pneumoniae. In order to investigate the TCS08 regulon, we have analyzed transcription profiles of mutants derived from S. pneumoniae R6 by microarray analysis. Since deletion mutants are often without a significant phenotype, we constructed a mutation in the histidine kinase HK08, T133P, in analogy to the phosphatase mutation T230P in the H box of the S. pneumoniae CiaH kinase described recently (D. Zahner, K. Kaminski, M. van der Linden, T. Mascher, M. Merai, and R. Hakenbeck, J. Mol. Microbiol. Biotechnol. 4:211-216, 2002). In addition, a deletion mutation was constructed in rr08, encoding the cognate response regulator. The most heavily suppressed genes in the hk08 mutant were spr0276 to spr0282, encoding a putative cellobiose phosphoenolpyruvate sugar phosphotransferase system (PTS). Whereas the R6 Sm(r) parent strain and the Deltarr08 mutant readily grew on cellobiose, the hk08 mutant and selected mutants with deletions in the PTS cluster did not, strongly suggesting that TCS08 is involved in the catabolism of cellobiose. Homologues of the TCS08 system were found in closely related streptococci and other gram-positive cocci. However, the genes spr0276 to spr0282, encoding the putative cellobiose PTS, represent a genomic island in S. pneumoniae and homologues were found in Streptococcus gordonii only, suggesting that this system might contribute to the pathogenicity potential of the pneumococcus.