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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0218

PubMed

25304507, Appl Environ Microbiol. 2015 Jan;81(1):31-9. doi: 10.1128/AEM.02257-14. Epub 2014 Oct 10.

Characterization method

enzyme activity assay

Genomic accession number

HF548278.1

Nucelotide position range

1-41173

Substrate

arabinose,arabinan

Loci

BN138_163-BN138_186

Species

termite gut metagenome/433724

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BN138_163 CCO20975.1 1 - 265 (-) HF548278.1:2-266 -
- BN138_164 CCO20976.1 491 - 2036 (-) HF548278.1:492-2037 -
- BN138_165 CCO20977.1 2047 - 5851 (-) HF548278.1:2048-5852 -
- BN138_166 CCO20978.1 5847 - 7506 (-) HF548278.1:5848-7507 -
- BN138_167 CCO20979.1 7507 - 9019 (-) HF548278.1:7508-9020 -
- BN138_168 CCO20980.1 9046 - 9733 (-) HF548278.1:9047-9734 -
- BN138_169 CCO20981.1 9737 - 10412 (-) HF548278.1:9738-10413 -
- BN138_170 CCO20982.1 10415 - 12107 (-) HF548278.1:10416-12108 -
- BN138_171 CCO20983.1 12140 - 13280 (-) HF548278.1:12141-13281 -
- BN138_172 CCO20984.1 13327 - 15247 (-) HF548278.1:13328-15248 -
- BN138_173 CCO20985.1 15259 - 15685 (-) HF548278.1:15260-15686 -
- BN138_174 CCO20986.1 15718 - 17497 (-) HF548278.1:15719-17498 -
- BN138_175 CCO20987.1 17515 - 20698 (-) HF548278.1:17516-20699 -
- BN138_176 CCO20988.1 20719 - 22462 (-) HF548278.1:20720-22463 -
- BN138_177 CCO20989.1 22469 - 25535 (-) HF548278.1:22470-25536 -
- BN138_178 CCO20990.1 25638 - 26337 (-) HF548278.1:25639-26338 -
- BN138_179 CCO20991.1 26356 - 28096 (-) HF548278.1:26357-28097 -
- BN138_180 CCO20992.1 28302 - 32367 (-) HF548278.1:28303-32368 -
- BN138_181 CCO20993.1 32594 - 34079 (+) HF548278.1:32595-34080 -
- BN138_182 CCO20994.1 34111 - 36985 (+) HF548278.1:34112-36986 -
- BN138_183 CCO20995.1 37328 - 38474 (+) HF548278.1:37329-38475 -
- BN138_184 CCO20996.1 38515 - 39766 (-) HF548278.1:38516-39767 -
- BN138_185 CCO20997.1 39954 - 40956 (+) HF548278.1:39955-40957 -
- BN138_186 CCO20998.1 40999 - 41173 (+) HF548278.1:41000-41174 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 2 - 265 (-) CDS No
- 492 - 2036 (-) CAZyme: GH51 Yes
- 2048 - 5851 (-) CAZyme: GH97|GH146 Yes
- 5848 - 7506 (-) other Yes
- 7508 - 9019 (-) other Yes
- 9047 - 9733 (-) other Yes
- 9738 - 10412 (-) STP: STP|NUDIX Yes
- 10416 - 12107 (-) TC: gnl|TC-DB|Q8AAV7|2.A.21.3.19 Yes
- 12141 - 13280 (-) other Yes
- 13328 - 15247 (-) CAZyme: GH43_4|GH43 Yes
- 15260 - 15685 (-) other Yes
- 15719 - 17497 (-) other Yes
- 17516 - 20698 (-) TC: gnl|TC-DB|Q93TH9|1.B.14.6.2 Yes
- 20720 - 22462 (-) other Yes
- 22470 - 25535 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 25639 - 26337 (-) other Yes
- 26357 - 28096 (-) other Yes
- 28303 - 32367 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 32595 - 34079 (+) CAZyme: GH43_4 Yes
- 34112 - 36985 (+) CAZyme: GH51|GH43_29 Yes
- 37329 - 38474 (+) CDS No
- 38516 - 39766 (-) STP: STP|AAA No
- 39955 - 40956 (+) CDS No
- 41000 - 41173 (+) CDS No

PUL ID

PUL0218

PubMed

25304507, Appl Environ Microbiol. 2015 Jan;81(1):31-9. doi: 10.1128/AEM.02257-14. Epub 2014 Oct 10.

Title

Investigating the function of an arabinan utilization locus isolated from a termite gut community.

Author

Arnal G, Bastien G, Monties N, Abot A, Anton Leberre V, Bozonnet S, O'Donohue M, Dumon C

Abstract

Biocatalysts are essential for the development of bioprocesses efficient for plant biomass degradation. Previously, a metagenomic clone containing DNA from termite gut microbiota was pinpointed in a functional screening that revealed the presence of arabinofuranosidase activity. Subsequent genetic and bioinformatic analysis revealed that the DNA fragment belonged to a member of the genus Bacteroides and encoded 19 open reading frames (ORFs), and annotation suggested the presence of hypothetical transporter and regulator proteins and others involved in the catabolism of pentose sugar. In this respect and considering the phenotype of the metagenomic clone, it was noted that among the ORFs, there are four putative arabinose-specific glycoside hydrolases, two from family GH43 and two from GH51. In this study, a thorough bioinformatics analysis of the metagenomic clone gene cluster has been performed and the four aforementioned glycoside hydrolases have been characterized. Together, the results provide evidence that the gene cluster is a polysaccharide utilization locus dedicated to the breakdown of the arabinan component in pectin and related substrates. Characterization of the two GH43 and the two GH51 glycoside hydrolases has revealed that each of these enzymes displays specific catalytic capabilities and that when these are combined the enzymes act synergistically, increasing the efficiency of arabinan degradation.