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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0466

PubMed

14973026, J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004.

Characterization method

clone and expression,enzyme activity assay,Northern Blot

Genomic accession number

NC_000964.3

Nucelotide position range

2938330-2948875

Substrate

arabinan

Loci

araABDLMNPQ-abfA

Species

Bacillus subtilis/1423

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

abfA BSU_28720 NP_390750.2 0 - 1503 (-) NC_000964.3:2938330-2939833 3.2.1.55
araQ BSU_28730 NP_390751.2 1521 - 2367 (-) NC_000964.3:2939851-2940697 -
araP BSU_28740 NP_390752.1 2367 - 3309 (-) NC_000964.3:2940697-2941639 -
araN BSU_28750 NP_390753.2 3344 - 4646 (-) NC_000964.3:2941674-2942976 -
egsA BSU_28760 NP_390754.1 4676 - 5861 (-) NC_000964.3:2943006-2944191 1.1.1.261
araL BSU_28770 NP_390755.1 5857 - 6676 (-) NC_000964.3:2944187-2945006 3.1.3.23
araD BSU_28780 NP_390756.1 6653 - 7343 (-) NC_000964.3:2944983-2945673 5.1.3.4
araB BSU_28790 NP_390757.1 7359 - 9042 (-) NC_000964.3:2945689-2947372 2.7.1.16
araA BSU_28800 NP_390758.2 9055 - 10546 (-) NC_000964.3:2947385-2948876 5.3.1.4

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

abfA 1 - 1503 (-) CAZyme: GH51 Yes
araQ 1522 - 2367 (-) TC: gnl|TC-DB|P94530|3.A.1.1.34 Yes
araP 2368 - 3309 (-) TC: gnl|TC-DB|P94529|3.A.1.1.34 Yes
araN 3345 - 4646 (-) TC: gnl|TC-DB|P94528|3.A.1.1.34 Yes
egsA 4677 - 5861 (-) CDS No
araL 5858 - 6676 (-) CDS No
araD 6654 - 7343 (-) CDS No
araB 7360 - 9042 (-) CDS No
araA 9056 - 10546 (-) CDS No

PUL ID

PUL0466

PubMed

14973026, J Bacteriol. 2004 Mar;186(5):1287-96. doi: 10.1128/JB.186.5.1287-1296.2004.

Title

Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.

Author

Raposo MP, Inacio JM, Mota LJ, de Sa-Nogueira I

Abstract

Bacillus subtilis produces hemicellulases capable of releasing arabinosyl oligomers and arabinose from plant cell walls. In this work, we characterize the transcriptional regulation of three genes encoding arabinan-degrading enzymes that are clustered with genes encoding enzymes that further catabolize arabinose. The abfA gene comprised in the metabolic operon araABDLMNPQ-abfA and the xsa gene located 23 kb downstream most probably encode alpha-L-arabinofuranosidases (EC 3.2.1.55). Here, we show that the abnA gene, positioned immediately upstream from the metabolic operon, encodes an endo-alpha-1,5-arabinanase (EC 3.2.1.99). Furthermore, by in vivo RNA studies, we inferred that abnA and xsa are monocistronic and are transcribed from sigma(A)-like promoters. Transcriptional fusion analysis revealed that the expression of the three arabinases is induced by arabinose and arabinan and is repressed by glucose. The levels of induction by arabinose and arabinan are higher during early postexponential growth, suggesting a temporal regulation. Moreover, the induction mechanism of these genes is mediated through negative control by the key regulator of arabinose metabolism, AraR. Thus, we analyzed AraR-DNA interactions by in vitro quantitative DNase I footprinting and in vivo analysis of single-base-pair substitutions within the promoter regions of xsa and abnA. The results indicate that transcriptional repression of the abfA and xsa genes is achieved by a tightly controlled mechanism but that the regulation of abnA is more flexible. We suggest that the expression of genes encoding extracellular degrading enzymes of arabinose-containing polysaccharides, transport systems, and intracellular enzymes involved in further catabolism is regulated by a coordinate mechanism triggered by arabinose via AraR.