Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0624

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKF010000001.1

Nucelotide position range

437271-467310

Substrate

xylan

Loci

HPS56_01555-HPS56_01605

Species

Prevotella sp. PMUR/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS56_01555 NPD91058.1 0 - 1143 (+) JABKKF010000001.1:437271-438414 -
- HPS56_01560 NPD91059.1 1611 - 5211 (+) JABKKF010000001.1:438882-442482 -
- HPS56_01565 NPD91060.1 5309 - 9560 (+) JABKKF010000001.1:442580-446831 -
- HPS56_01570 NPD91061.1 9575 - 12287 (+) JABKKF010000001.1:446846-449558 -
- HPS56_01575 NPD91062.1 12363 - 15657 (+) JABKKF010000001.1:449634-452928 -
- HPS56_01580 NPD91063.1 15672 - 17397 (+) JABKKF010000001.1:452943-454668 -
- HPS56_01585 NPD91064.1 17433 - 19506 (+) JABKKF010000001.1:454704-456777 -
- HPS56_01590 NPD91065.1 19530 - 21225 (+) JABKKF010000001.1:456801-458496 -
- HPS56_01595 NPD91066.1 21258 - 24543 (+) JABKKF010000001.1:458529-461814 -
- HPS56_01600 NPD91067.1 24564 - 26331 (+) JABKKF010000001.1:461835-463602 -
- HPS56_01605 NPD91068.1 26371 - 30040 (+) JABKKF010000001.1:463642-467311 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1143 (+) CAZyme: GH43_24| GH43_24 Yes
- 1612 - 5211 (+) other Yes
- 5310 - 9560 (+) CAZyme: GH43_19| GH43_19| GH51|GH51 Yes
- 9576 - 12287 (+) other Yes
- 12364 - 15657 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 15673 - 17397 (+) TC: gnl|TC-DB|Q5LEN2|8.A.46.1.4 Yes
- 17434 - 19506 (+) other Yes
- 19531 - 21225 (+) other Yes
- 21259 - 24543 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 24565 - 26331 (+) other Yes
- 26372 - 30040 (+) CAZyme: GH128|GH128 Yes

PUL ID

PUL0624

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.