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Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0666

PubMed

34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.

Characterization method

UHPLC-MS,RNA-seq,RT-qPCR

Genomic accession number

NC_004663.1

Nucelotide position range

4732770-4741675

Substrate

pectin

Loci

BT_3654-BT_3658

Species

Bacteroides thetaiotaomicron VPI-5482/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS18435 WP_008767049.1 0 - 2337 (+) NC_004663.1:4732770-4735107 3.2.1.23
- BT_RS18440 WP_008767048.1 2407 - 3379 (+) NC_004663.1:4735177-4736149 -
- BT_RS18445 WP_008767047.1 3420 - 5856 (+) NC_004663.1:4736190-4738626 -
- BT_RS18450 WP_011108921.1 5926 - 8404 (+) NC_004663.1:4738696-4741174 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2337 (+) CAZyme: CBM32| GH35|GH35 No
- 2408 - 3379 (+) CAZyme: GH43_19| GH43_19|3.2.1.55 No
- 3421 - 5856 (+) CAZyme: GH43_9|GH43_19| GH43_19|CBM91|GH43_9 No
- 5927 - 8404 (+) CAZyme: GH51|GH51 No

PUL ID

PUL0666

PubMed

34420703, Carbohydr Polym. 2021 Nov 15;272:118534. doi: 10.1016/j.carbpol.2021.118534. Epub 2021 Aug 6.

Title

Discrete genetic loci in human gut Bacteroides thetaiotaomicron confer pectin metabolism.

Author

Li M, Li S, Guo X, Guo C, Wang Y, Du Z, Zhang Z, Xie C, Ding K

Abstract

Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.