Home Repository Download Help About Statistics Taxonomy Blastx
Links
PULDB
CAZy
dbCAN2
dbCAN-seq
run_dbcan
CAZypedia
PlantCAZyme
Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0625

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKF010000013.1

Nucelotide position range

29296-73074

Substrate

xylan

Loci

HPS56_11565-HPS56_11655

Species

Prevotella sp. PMUR/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS56_11565 NPD92966.1 0 - 1650 (-) JABKKF010000013.1:29296-30946 -
- HPS56_11570 NPD92967.1 1710 - 4212 (-) JABKKF010000013.1:31006-33508 -
- HPS56_11575 NPD92968.1 4226 - 6323 (-) JABKKF010000013.1:33522-35619 -
- HPS56_11580 NPD92969.1 6359 - 8264 (-) JABKKF010000013.1:35655-37560 -
- HPS56_11585 NPD92970.1 8318 - 9689 (-) JABKKF010000013.1:37614-38985 -
- HPS56_11590 NPD92971.1 9695 - 11561 (-) JABKKF010000013.1:38991-40857 -
- HPS56_11595 NPD92972.1 11576 - 14033 (-) JABKKF010000013.1:40872-43329 -
- HPS56_11600 NPD92973.1 14042 - 16565 (-) JABKKF010000013.1:43338-45861 -
- HPS56_11605 - 17707 - 19258 (-) JABKKF010000013.1:47003-48554 -
- HPS56_11610 NPD92974.1 19439 - 22811 (-) JABKKF010000013.1:48735-52107 -
- HPS56_11615 NPD92975.1 23318 - 27197 (-) JABKKF010000013.1:52614-56493 -
- HPS56_11620 NPD92976.1 27591 - 28833 (-) JABKKF010000013.1:56887-58129 -
- HPS56_11625 NPD92977.1 28861 - 30370 (-) JABKKF010000013.1:58157-59666 -
- HPS56_11630 NPD92978.1 30366 - 32043 (-) JABKKF010000013.1:59662-61339 -
- HPS56_11635 NPD92979.1 32052 - 35208 (-) JABKKF010000013.1:61348-64504 -
- HPS56_11640 NPD92980.1 35207 - 36827 (-) JABKKF010000013.1:64503-66123 -
- HPS56_11645 NPD92981.1 36867 - 38796 (-) JABKKF010000013.1:66163-68092 -
- HPS56_11650 NPD92982.1 38827 - 40513 (-) JABKKF010000013.1:68123-69809 -
- HPS56_11655 NPD92983.1 40524 - 43779 (-) JABKKF010000013.1:69820-73075 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1650 (-) CAZyme: CBM91|GH43_12| GH43_12 Yes
- 1711 - 4212 (-) CAZyme: GH115| GH115 Yes
- 4227 - 6323 (-) CAZyme: GH43_10| GH43_10|CBM91 Yes
- 6360 - 8264 (-) CAZyme: GH97|GH97 Yes
- 8319 - 9689 (-) CAZyme: GH43_29|GH43_29| CBM6|CBM6 Yes
- 9696 - 11561 (-) CAZyme: CE1|CE0|CBM48|CE6|CE1 Yes
- 11577 - 14033 (-) CAZyme: GH95| GH95 Yes
- 14043 - 16565 (-) CAZyme: GH115| GH115 Yes
- 19440 - 22811 (-) other Yes
- 23319 - 27197 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 27592 - 28833 (-) CAZyme: CE1|CE1 Yes
- 28862 - 30370 (-) CAZyme: GH30_8|GH30_8 Yes
- 30367 - 32043 (-) other Yes
- 32053 - 35208 (-) other Yes
- 35208 - 36827 (-) other Yes
- 36868 - 38796 (-) other Yes
- 38828 - 40513 (-) other Yes
- 40525 - 43779 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes

PUL ID

PUL0625

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.