Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0230 RT-PCR, enzyme activity assay, clone, enzyme kinetic analysis, thin layer chromatography, crystallization maltooligosaccharide Lactobacillus acidophilus 22685275, 32444471
Enzymology and structure of the GH13_31 glucan 1,6-alpha-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM. An 1,4-alpha-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus. J Bacteriol. 2012 Aug;194(16):4249-59. doi: 10.1128/JB.00622-12. Epub 2012 Jun 8. Appl Environ Microbiol. 2020 Jul 20;86(15). pii: AEM.00661-20. doi: 10.1128/AEM.00661-20. Print 2020 Jul 20.
2012 Aug,2020 Jul 20 degradation 12 4 GH65, CBM34, GH13, GH13_20
PUL0267 RT-qPCR glycogen Lactobacillus acidophilus 23879596
A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon. Mol Microbiol. 2013 Sep;89(6):1187-200. doi: 10.1111/mmi.12338. Epub 2013 Aug 16.
2013 Sep biosynthesis 10 4 GH13_9, CBM48, GH13, GT5, GT35, GH13_39, CBM34, GH13
PUL0327 microarray, gas chromatography and mass spectrometry, gene deletion mutant and growth assay, complementation study maltodextrin Enterococcus faecalis 28455338, 32680872
Enzymes Required for Maltodextrin Catabolism in Enterococcus faecalis Exhibit Novel Activities. Enterococcus faecalis Maltodextrin Gene Regulation by Combined Action of Maltose Gene Regulator MalR and Pleiotropic Regulator CcpA. Appl Environ Microbiol. 2017 Jun 16;83(13). pii: AEM.00038-17. doi: 10.1128/AEM.00038-17. Print 2017 Jul 1. Appl Environ Microbiol. 2020 Sep 1;86(18). pii: AEM.01147-20. doi: 10.1128/AEM.01147-20. Print 2020 Sep 1.
2017 Jul 1,2020 Sep 1 degradation 6 3 GH13, CBM34, GH13_20
PUL0361 enzyme activity assay starch, maltodextrin Thermotoga maritima 10972187
Thermotoga maritima AglA, an extremely thermostable NAD+-, Mn2+-, and thiol-dependent alpha-glucosidase. Extremophiles. 2000 Aug;4(4):189-200. doi: 10.1007/pl00010711.
2000 Aug degradation 6 3 GH13_20, CBM34, GH13, GH4
PUL0371 enzyme activity assay cyclomaltodextrin Thermococcus sp. B1001 11489857
Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001. J Bacteriol. 2001 Sep;183(17):5050-7. doi: 10.1128/jb.183.17.5050-5057.2001.
2001 Sep degradation 5 1 CBM34, GH13, GH13_20
PUL0590 qRT-PCR, gene deletion mutant and growth assay, microarray maltose, maltodextrin Enterococcus faecium 23951303
A LacI-family regulator activates maltodextrin metabolism of Enterococcus faecium. PLoS One. 2013 Aug 7;8(8):e72285. doi: 10.1371/journal.pone.0072285. eCollection 2013.
2013 degradation 5 1 CBM34, GH13_20
PUL0593 Northern Blot maltose, maltotriose Thermoanaerobacterium thermosulfurigenes 8576036
Molecular analysis of the amy gene locus of Thermoanaerobacterium thermosulfurigenes EM1 encoding starch-degrading enzymes and a binding protein-dependent maltose transport system. J Bacteriol. 1996 Feb;178(4):1039-46. doi: 10.1128/jb.178.4.1039-1046.1996.
1996 Feb degradation 6 2 GH13_39, CBM34, GH13, CBM20, GH13_2, CBM20