Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0166 enzyme activity assay, RT-PCR starch Bacteroides fragilis 16788175
Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis: Regulation by carbon source and oxygen. J Bacteriol. 2006 Jul;188(13):4663-72. doi: 10.1128/JB.00125-06.
2006 Jul degradation 5 1 GH13_10, GH13
PUL0174 RT-PCR, enzyme activity assay, enzymatic product analysis starch Kribbella flavida 27302067
Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1-->6)-alpha-nigerosyl. J Biol Chem. 2016 Aug 5;291(32):16438-47. doi: 10.1074/jbc.M116.727305. Epub 2016 Jun 14.
2016 Aug 5 degradation 3 2 CBM20, GH31, CBM35, GH31
PUL0268 Northern Blot, promoter assay starch Geobacillus kaustophilus 23793634
Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426. Appl Environ Microbiol. 2013 Sep;79(17):5151-8. doi: 10.1128/AEM.01506-13. Epub 2013 Jun 21.
2013 Sep degradation 5 1 GH13_1, GH13
PUL0361 enzyme activity assay starch, maltodextrin Thermotoga maritima 10972187
Thermotoga maritima AglA, an extremely thermostable NAD+-, Mn2+-, and thiol-dependent alpha-glucosidase. Extremophiles. 2000 Aug;4(4):189-200. doi: 10.1007/pl00010711.
2000 Aug degradation 6 3 GH13_20, CBM34, GH13, GH4
PUL0421 microarray starch Bacteroides ovatus 22205877
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 2011 Dec;9(12):e1001221. doi: 10.1371/journal.pbio.1001221. Epub 2011 Dec 20.
2011 Dec degradation 7 3 GH13, GH13_10, GH97, GH13
PUL0469 microarray, qPCR starch Bacteroides thetaiotaomicron 18996345
Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont. Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.
2008 Nov 13 degradation 8 3 GH97
PUL0473 growth assay alpha-glucan, starch Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 8 3 CBM26, GH13, GH97, GH13
PUL0478 growth assay alpha-glucan, starch Flavobacterium johnsoniae 19717629
Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl Environ Microbiol. 2009 Nov;75(21):6864-75. doi: 10.1128/AEM.01495-09. Epub 2009 Aug 28.
2009 Nov degradation 8 4 GH2, GH92, GH92, GH125
PUL0589 Western Blot, enzyme activity assay, thin layer chromatography starch Streptococcus mutans 23930155
The malQ gene is essential for starch metabolism in Streptococcus mutans. J Oral Microbiol. 2013 Aug 6;5. doi: 10.3402/jom.v5i0.21285. Print 2013.
2013 degradation 3 2 GT35, GH77
PUL0595 enzyme activity assay, qPCR, crystallization starch [Eubacterium] rectale 25388295
Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19.
2015 Jan degradation 4 1 CBM26, GH13_41, CBM82, GH13, CBM41, CBM83
PUL0596 enzyme activity assay, qPCR, crystallization starch [Eubacterium] rectale 25388295
Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol. 2015 Jan;95(2):209-30. doi: 10.1111/mmi.12859. Epub 2014 Dec 19.
2015 Jan degradation 4 1 GH13_36