PUL ID

PUL0469

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Characterization method

microarray,qPCR

Genomic accession number

AE015928.1

Nucelotide position range

4800873-4816693

Substrate

starch

Loci

BT3698-BT3705

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

susG BT_3698 AAO78803.1 0 - 2079 (-) AE015928.1:4800873-4802952 -
susF BT_3699 AAO78804.1 2188 - 3646 (-) AE015928.1:4803061-4804519 -
susE BT_3700 AAO78805.1 3671 - 4835 (-) AE015928.1:4804544-4805708 -
susD BT_3701 AAO78806.1 4869 - 6525 (-) AE015928.1:4805742-4807398 -
susC BT_3702 AAO78807.1 6546 - 9558 (-) AE015928.1:4807419-4810431 -
susB BT_3703 AAO78808.1 9712 - 11929 (-) AE015928.1:4810585-4812802 -
susA BT_3704 AAO78809.1 12125 - 13979 (-) AE015928.1:4812998-4814852 -
susR BT_3705 AAO78810.1 14072 - 15821 (+) AE015928.1:4814945-4816694 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

susG 1 - 2079 (-) TC: gnl|TC-DB|Q05839|8.A.9.1.1 Yes
susF 2189 - 3646 (-) other Yes
susE 3672 - 4835 (-) other Yes
susD 4870 - 6525 (-) TC: gnl|TC-DB|Q8A1G2|8.A.46.1.1 Yes
susC 6547 - 9558 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
susB 9713 - 11929 (-) CAZyme: GH97 Yes
susA 12126 - 13979 (-) TC: gnl|TC-DB|Q07837|8.A.9.1.2 Yes
susR 14073 - 15821 (+) CDS No

PUL ID

PUL0469

PubMed

18996345, Cell Host Microbe. 2008 Nov 13;4(5):447-57. doi: 10.1016/j.chom.2008.09.007.

Title

Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Author

Martens EC, Chiang HC, Gordon JI

Abstract

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profiling and genetic tests were used to define the mechanisms underlying host glycan foraging in vivo and in vitro. PULs that target all major classes of host glycans were identified. However, mucin O-glycans are the principal host substrate foraged in vivo. Simultaneous deletion of five genes encoding ECF-sigma transcription factors, which activate mucin O-glycan utilization, produces defects in bacterial persistence in the gut and in mother-to-offspring transmission. Thus, PUL-mediated glycan catabolism is an important component in gut colonization and may impact microbiota ecology.