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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0647

PubMed

33603728, Front Microbiol. 2021 Feb 2;12:636684. doi: 10.3389/fmicb.2021.636684. eCollection 2021.

Characterization method

qPCR

Genomic accession number

NC_004350.2

Nucelotide position range

1459786-1467814

Substrate

sucrose

Loci

SMU_1535-SMU_1539

Species

Streptococcus mutans UA159/1309

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SMU_RS06970 WP_002262947.1 0 - 2397 (-) NC_004350.2:1459786-1462183 -
glgA SMU_RS06975 WP_002262948.1 2427 - 3858 (-) NC_004350.2:1462213-1463644 2.4.1.21
glgD SMU_RS06980 WP_002262949.1 3854 - 4988 (-) NC_004350.2:1463640-1464774 2.7.7.27
- SMU_RS06985 WP_002262950.1 4977 - 6117 (-) NC_004350.2:1464763-1465903 2.7.7.27
glgB SMU_RS06990 WP_002262951.1 6142 - 8029 (-) NC_004350.2:1465928-1467815 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 2397 (-) CAZyme: GT35| GT35 No
glgA 2428 - 3858 (-) CAZyme: GT5|GT5 No
glgD 3855 - 4988 (-) CDS No
- 4978 - 6117 (-) CDS No
glgB 6143 - 8029 (-) CAZyme: CBM48| GH13_9|GH13_9 No

PUL ID

PUL0647

PubMed

33603728, Front Microbiol. 2021 Feb 2;12:636684. doi: 10.3389/fmicb.2021.636684. eCollection 2021.

Title

The Route of Sucrose Utilization by Streptococcus mutans Affects Intracellular Polysaccharide Metabolism.

Author

Costa Oliveira BE, Ricomini Filho AP, Burne RA, Zeng L

Abstract

Streptococcus mutans converts extracellular sucrose (Suc) into exopolysaccharides (EPS) by glucosyl-transferase and fructosyl-transferase enzymes and internalizes Suc for fermentation through the phosphotransferase system (PTS). Here, we examined how altering the routes for sucrose utilization impacts intracellular polysaccharide [IPS; glycogen, (glg)] metabolism during carbohydrate starvation. Strain UA159 (WT), a mutant lacking all exo-enzymes for sucrose utilization (MMZ952), and a CcpA-deficient mutant (∆ccpA) were cultured with sucrose or a combination of glucose and fructose, followed by carbohydrate starvation. At baseline (0h), and after 4 and 24h of starvation, cells were evaluated for mRNA levels of the glg operon, IPS storage, glucose-1-phosphate (G1P) concentrations, viability, and PTS activities. A pH drop assay was performed in the absence of carbohydrates at the baseline to measure acid production. We observed glg operon activation in response to starvation (p<0.05) in all strains, however, such activation was significantly delayed and reduced in magnitude when EPS synthesis was involved (p<0.05). Enhanced acidification and greater G1P concentrations were observed in the sucrose-treated group, but mostly in strains capable of producing EPS (p<0.05). Importantly, only the WT exposed to sucrose was able to synthesize IPS during starvation. Contrary to CcpA-proficient strains, IPS was progressively degraded during starvation in ∆ccpA, which also showed increased glg operon expression and greater PTS activities at baseline. Therefore, sucrose metabolism by secreted enzymes affects the capacity of S. mutans in synthesizing IPS and converting it into organic acids, without necessarily inducing greater expression of the glg operon.