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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0557

PubMed

25567280, Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995.

Characterization method

gene deletion mutant and growth assay

Genomic accession number

AE015928.1

Nucelotide position range

3262908-3288188

Substrate

alpha-mannan

Loci

BT2620-BT2632

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_2620 AAO77727.1 0 - 1950 (-) AE015928.1:3262908-3264858 -
- BT_2621 AAO77728.1 1975 - 3286 (-) AE015928.1:3264883-3266194 -
- BT_2622 AAO77729.1 3294 - 5463 (-) AE015928.1:3266202-3268371 -
- BT_2623 AAO77730.1 5559 - 7113 (-) AE015928.1:3268467-3270021 -
- BT_2624 AAO77731.1 7128 - 8094 (-) AE015928.1:3270036-3271002 -
- BT_2625 AAO77732.1 8105 - 10088 (-) AE015928.1:3271013-3272996 -
- BT_2626 AAO77733.1 10098 - 13203 (-) AE015928.1:3273006-3276111 -
- BT_2627 AAO77734.1 13236 - 14541 (-) AE015928.1:3276144-3277449 -
- BT_2628 AAO77735.1 15031 - 19069 (-) AE015928.1:3277939-3281977 -
- BT_2629 AAO77736.1 19279 - 21562 (+) AE015928.1:3282187-3284470 -
- BT_2630 AAO77737.1 21615 - 22566 (+) AE015928.1:3284523-3285474 -
- BT_2631 AAO77738.1 22618 - 23776 (+) AE015928.1:3285526-3286684 -
- BT_2632 AAO77739.1 23835 - 25281 (+) AE015928.1:3286743-3288189 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1950 (-) CAZyme: GH97 Yes
- 1976 - 3286 (-) other Yes
- 3295 - 5463 (-) CAZyme: GH67 Yes
- 5560 - 7113 (-) CAZyme: GH76 Yes
- 7129 - 8094 (-) other Yes
- 8106 - 10088 (-) other Yes
- 10099 - 13203 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 13237 - 14541 (-) other Yes
- 15032 - 19069 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 19280 - 21562 (+) CAZyme: GH92 Yes
- 21616 - 22566 (+) other Yes
- 22619 - 23776 (+) CAZyme: GH76 Yes
- 23836 - 25281 (+) CAZyme: GH125 Yes

PUL ID

PUL0557

PubMed

25567280, Nature. 2015 Jan 8;517(7533):165-169. doi: 10.1038/nature13995.

Title

Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.

Author

Cuskin F, Lowe EC, Temple MJ, Zhu Y, Cameron E, Pudlo NA, Porter NT, Urs K, Thompson AJ, Cartmell A, Rogowski A, Hamilton BS, Chen R, Tolbert TJ, Piens K, Bracke D, Vervecken W, Hakki Z, Speciale G, Munoz-Munoz JL, Day A, Pena MJ, McLean R, Suits MD, Boraston AB, Atherly T, Ziemer CJ, Williams SJ, Davies GJ, Abbott DW, Martens EC, Gilbert HJ

Abstract

Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall alpha-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast alpha-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of alpha-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.