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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0045

PubMed

26112186, Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481.

Characterization method

qRT-PCR

Genomic accession number

DS264583.1

Nucelotide position range

286501-307432

Substrate

arabinoxylan

Loci

bacova_04385-bacova_04394

Species

Bacteroides ovatus/28116

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BACOVA_04384 EDO10004.1 0 - 19 (+) DS264583.1:286501-286520 -
- BACOVA_04385 EDO10005.1 0 - 2136 (-) DS264583.1:286501-288637 -
- BACOVA_04386 EDO10006.1 2301 - 3279 (-) DS264583.1:288802-289780 -
- BACOVA_04387 EDO10007.1 3305 - 4436 (-) DS264583.1:289806-290937 -
gph BACOVA_04388 EDO10008.1 4475 - 5891 (-) DS264583.1:290976-292392 -
- BACOVA_04389 EDO10009.1 5907 - 7686 (-) DS264583.1:292408-294187 -
- BACOVA_04390 EDO10010.1 7898 - 10121 (-) DS264583.1:294399-296622 -
- BACOVA_04391 EDO10011.1 10145 - 11549 (-) DS264583.1:296646-298050 -
- BACOVA_04392 EDO10012.1 11563 - 13210 (-) DS264583.1:298064-299711 -
- BACOVA_04393 EDO10013.1 13229 - 16403 (-) DS264583.1:299730-302904 -
- BACOVA_04394 EDO10014.1 16798 - 20932 (+) DS264583.1:303299-307433 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 19 (+) CDS No
- 1 - 2136 (-) CAZyme: GH67 Yes
- 2302 - 3279 (-) CAZyme: GH43_1|GH43 Yes
- 3306 - 4436 (-) CAZyme: GH10 Yes
gph 4476 - 5891 (-) TC: gnl|TC-DB|P94488|2.A.2.3.2 Yes
- 5908 - 7686 (-) other Yes
- 7899 - 10121 (-) CAZyme: CBM4|GH10 Yes
- 10146 - 11549 (-) other Yes
- 11564 - 13210 (-) other Yes
- 13230 - 16403 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 16799 - 20932 (+) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607,DBD-SUPERFAMILY|0035607 No

PUL ID

PUL0045

PubMed

26112186, Nat Commun. 2015 Jun 26;6:7481. doi: 10.1038/ncomms8481.

Title

Glycan complexity dictates microbial resource allocation in the large intestine.

Author

Rogowski A, Briggs JA, Mortimer JC, Tryfona T, Terrapon N, Lowe EC, Basle A, Morland C, Day AM, Zheng H, Rogers TE, Thompson P, Hawkins AR, Yadav MP, Henrissat B, Martens EC, Dupree P, Gilbert HJ, Bolam DN

Abstract

The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota.