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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0161

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Characterization method

mass spectrometry,sequence homology analysis

Genomic accession number

NC_004663.1

Nucelotide position range

3262908-3301755

Substrate

alpha-mannan

Loci

BT_2620-BT_2648

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_RS13250 WP_011108399.1 0 - 1950 (-) NC_004663.1:3262908-3264858 -
- BT_RS13255 WP_164927909.1 1975 - 3295 (-) NC_004663.1:3264883-3266203 -
- BT_RS13260 WP_011108401.1 3294 - 5463 (-) NC_004663.1:3266202-3268371 -
- BT_RS13265 WP_011108402.1 5559 - 7113 (-) NC_004663.1:3268467-3270021 -
- BT_RS13270 WP_011108403.1 7128 - 8094 (-) NC_004663.1:3270036-3271002 -
- BT_RS13275 WP_011108404.1 8105 - 10088 (-) NC_004663.1:3271013-3272996 -
- BT_RS13280 WP_162303101.1 10098 - 13197 (-) NC_004663.1:3273006-3276105 -
- BT_RS13285 WP_011108406.1 13236 - 14541 (-) NC_004663.1:3276144-3277449 -
- BT_RS13290 WP_011108407.1 15031 - 19069 (-) NC_004663.1:3277939-3281977 -
- BT_RS13295 WP_011108408.1 19279 - 21562 (+) NC_004663.1:3282187-3284470 -
- BT_RS13300 WP_004304952.1 21615 - 22566 (+) NC_004663.1:3284523-3285474 -
- BT_RS13305 WP_011108409.1 22618 - 23776 (+) NC_004663.1:3285526-3286684 -
- BT_RS13310 WP_011108410.1 23835 - 25281 (+) NC_004663.1:3286743-3288189 -
- BT_RS13315 WP_113760760.1 25754 - 25838 (-) NC_004663.1:3288662-3288746 -
- BT_RS13320 WP_011108412.1 26067 - 27060 (-) NC_004663.1:3288975-3289968 -
- BT_RS13325 WP_158257884.1 28084 - 28357 (-) NC_004663.1:3290992-3291265 -
- BT_RS13330 WP_162303082.1 28429 - 29455 (-) NC_004663.1:3291337-3292363 -
- BT_RS13335 WP_011108420.1 30929 - 32735 (+) NC_004663.1:3293837-3295643 -
- BT_RS13340 WP_004323215.1 32781 - 33057 (+) NC_004663.1:3295689-3295965 -
- BT_RS13345 WP_011108421.1 33151 - 34912 (+) NC_004663.1:3296059-3297820 -
- BT_RS13350 WP_004323218.1 35196 - 35409 (+) NC_004663.1:3298104-3298317 -
- BT_RS13355 WP_073343634.1 35429 - 35639 (+) NC_004663.1:3298337-3298547 -
- BT_RS13360 WP_011108422.1 35657 - 36740 (+) NC_004663.1:3298565-3299648 -
- BT_RS13365 WP_011108423.1 36761 - 36983 (+) NC_004663.1:3299669-3299891 -
- BT_RS13370 WP_011108424.1 37027 - 38134 (+) NC_004663.1:3299935-3301042 -
- BT_RS13375 WP_011108425.1 38146 - 38147 (+) NC_004663.1:3301054-3301055 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1950 (-) CAZyme: GH97 Yes
- 1976 - 3295 (-) other Yes
- 3295 - 5463 (-) CAZyme: GH67 Yes
- 5560 - 7113 (-) CAZyme: GH76 Yes
- 7129 - 8094 (-) other Yes
- 8106 - 10088 (-) other Yes
- 10099 - 13197 (-) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 13237 - 14541 (-) other Yes
- 15032 - 19069 (-) TF: DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0035607 Yes
- 19280 - 21562 (+) CAZyme: GH92 Yes
- 21616 - 22566 (+) other Yes
- 22619 - 23776 (+) CAZyme: GH76 Yes
- 23836 - 25281 (+) CAZyme: GH125 Yes
- 25755 - 25838 (-) other Yes
- 26068 - 27060 (-) other Yes
- 28085 - 28357 (-) other Yes
- 28430 - 29455 (-) other Yes
- 30930 - 32735 (+) other Yes
- 32782 - 33057 (+) other Yes
- 33152 - 34912 (+) TC: gnl|TC-DB|Q07711|3.A.7.7.1 Yes
- 35197 - 35409 (+) CDS No
- 35430 - 35639 (+) CDS No
- 35658 - 36740 (+) CDS No
- 36762 - 36983 (+) CDS No
- 37028 - 38134 (+) CDS No
- 38147 - 38147 (+) CDS No

PUL ID

PUL0161

PubMed

30246424, Environ Microbiol. 2018 Nov;20(11):4127-4140. doi: 10.1111/1462-2920.14414. Epub 2018 Oct 16.

Title

Alpha- and beta-mannan utilization by marine Bacteroidetes.

Author

Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avci B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H

Abstract

Marine microscopic algae carry out about half of the global carbon dioxide fixation into organic matter. They provide organic substrates for marine microbes such as members of the Bacteroidetes that degrade algal polysaccharides using carbohydrate-active enzymes (CAZymes). In Bacteroidetes genomes CAZyme encoding genes are mostly grouped in distinct regions termed polysaccharide utilization loci (PULs). While some studies have shown involvement of PULs in the degradation of algal polysaccharides, the specific substrates are for the most part still unknown. We investigated four marine Bacteroidetes isolated from the southern North Sea that harbour putative mannan-specific PULs. These PULs are similarly organized as PULs in human gut Bacteroides that digest alpha- and beta-mannans from yeasts and plants respectively. Using proteomics and defined growth experiments with polysaccharides as sole carbon sources we could show that the investigated marine Bacteroidetes express the predicted functional proteins required for alpha- and beta-mannan degradation. Our data suggest that algal mannans play an as yet unknown important role in the marine carbon cycle, and that biochemical principles established for gut or terrestrial microbes also apply to marine bacteria, even though their PULs are evolutionarily distant.