PUL ID

PUL0381

PubMed

14983042, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2524-9. doi: 10.1073/pnas.0308707101.

Characterization method

microarray, gene deletion mutant and growth assay

Genomic accession number

NC_002505.1

Nucelotide position range

1355388-1361117

Substrate

chitin

Loci

VC1280-VC1286

Species

Vibrio cholerae/666

Degradation or Biosynthesis

degradation

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

cod 1 - 1296 (-) CAZyme: CBM12|CE4 Yes
- 1539 - 1844 (+) TC: gnl|TC-DB|Q9KSH5|4.A.3.2.6 Yes
- 1932 - 3263 (+) TC: gnl|TC-DB|Q9KSH4|4.A.3.2.6 Yes
- 3310 - 3645 (+) TC: gnl|TC-DB|Q9KSH3|4.A.3.2.6 Yes
- 3627 - 4949 (+) CAZyme: GH4 Yes
chbG 4972 - 5730 (+) CDS No

PUL ID

PUL0381

PubMed

14983042, Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2524-9. doi: 10.1073/pnas.0308707101.

Title

The Vibrio cholerae chitin utilization program.

Author

Meibom KL, Li XB, Nielsen AT, Wu CY, Roseman S, Schoolnik GK

Abstract

Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc)(2-6), GlcNAc, or the glucosamine dimer (GlcN)2 identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, including a (GlcNAc)2 catabolic operon, two extracellular chitinases, a chitoporin, and a PilA-containing type IV pilus, designated ChiRP (chitin-regulated pilus) that confers a significant growth advantage to V. cholerae on a chitin surface; (ii) GlcNAc causes the coordinate expression of genes involved with chitin chemotaxis and adherence and with the transport and assimilation of GlcNAc; (iii) (GlcN)2 induces genes required for the transport and catabolism of nonacetylated chitin residues; and (iv) the constitutively expressed MSHA pilus facilitates adhesion to the chitin surface independent of surface chemistry. Collectively, these results provide a global portrait of a complex, multistage V. cholerae program for the efficient utilization of chitin.