Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0027 Northern Blot, gene deletion mutant and growth assay alginate Azotobacter vinelandii 10352233
Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene. 1999 May 31;232(2):217-22. doi: 10.1016/s0378-1119(99)00119-5.
1999 May 31 biosynthesis 5 1 PL5_1
PUL0051 sequence homology analysis alginate Cellulophaga lytica 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 6 2 PL6_1, PL6, PL17_2, PL17
PUL0052 sequence homology analysis alginate Maricaulis maris 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 10 2 PL6, PL6_1, PL17, PL17_2
PUL0053 sequence homology analysis alginate Stenotrophomonas maltophilia 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 8 2 PL6_1, PL6, PL17, PL17_2
PUL0054 sequence homology analysis alginate Alteromonas macleodii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 9 2 PL6, PL6_1, PL17_2, PL17
PUL0055 sequence homology analysis alginate Bacteroides sp. 1_1_30 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 7 2 PL17, PL17_2, PL6, PL6_1
PUL0056 sequence homology analysis alginate Bacteroides eggerthii 29795267
Ancient acquisition of "alginate utilization loci" by human gut microbiota. Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.
2018 May 23 degradation 5 3 PL6_1, PL6, PL17, PL17_2
PUL0126 growth assay, sequence homology analysis alginate, ulvan Alteromonas sp. 76-1 30936857
Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019.
2019 degradation 8 2 PL7, PL7_5, PL6, PL6_1
PUL0127 growth assay, sequence homology analysis alginate, ulvan Alteromonas sp. 76-1 30936857
Adaptations of Alteromonas sp. 76-1 to Polysaccharide Degradation: A CAZyme Plasmid for Ulvan Degradation and Two Alginolytic Systems. Front Microbiol. 2019 Mar 18;10:504. doi: 10.3389/fmicb.2019.00504. eCollection 2019.
2019 degradation 12 3 PL7, PL7_5, CBM32, PL6_3, PL6, PL7, PL7_5, PL6, PL6_1
PUL0150 sequence homology analysis alginate Pseudoalteromonas carrageenovora 30524390
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018.
2018 degradation 14 3 PL6, PL6_1, PL17, PL17_2, PL6_3
PUL0151 sequence homology analysis, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 30524390, 32585009
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2018,2020 Aug 20 degradation 12 2 PL7, PL17_2, PL17
PUL0152 sequence homology analysis alginate Pseudoalteromonas atlantica 30524390
Evolutionary Evidence of Algal Polysaccharide Degradation Acquisition by Pseudoalteromonas carrageenovora 9(T) to Adapt to Macroalgal Niches. Front Microbiol. 2018 Nov 22;9:2740. doi: 10.3389/fmicb.2018.02740. eCollection 2018.
2018 degradation 8 1 PL6, PL6_1
PUL0155 enzyme activity assay alginate Agrobacterium fabrum 16545947
A biosystem for alginate metabolism in Agrobacterium tumefaciens strain C58: molecular identification of Atu3025 as an exotype family PL-15 alginate lyase. Res Microbiol. 2006 Sep;157(7):642-9. doi: 10.1016/j.resmic.2006.02.006. Epub 2006 Mar 2.
2006 Sep degradation 8 1 PL15_1
PUL0199 enzyme activity assay, liquid chromatography and mass spectrometry alginate Saccharophagus degradans 26458373
Putative Alginate Assimilation Process of the Marine Bacterium Saccharophagus degradans 2-40 Based on Quantitative Proteomic Analysis. Mar Biotechnol (NY). 2016 Feb;18(1):15-23. doi: 10.1007/s10126-015-9667-3. Epub 2015 Oct 12.
2016 Feb degradation 17 7 CBM16, PL18, CBM32, PL6_1, PL6, PL6_1, PL6, PL17, PL17_2, PL6_1, PL6, PL7_5
PUL0216 Western Blot, enzyme activity assay, RT-PCR, microarray alginate Sphingomonas sp. 24816607
Alginate-dependent gene expression mechanism in Sphingomonas sp. strain A1. J Bacteriol. 2014 Jul;196(14):2691-700. doi: 10.1128/JB.01666-14. Epub 2014 May 9.
2014 Jul degradation 10 1 PL7, PL5, PL15_1
PUL0235 proteome fractionation, mass spectrometry, target decoy database analysis alginate Gramella forsetii 24522261
Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4. Epub 2014 Feb 13.
2014 Jul degradation 20 7 PL7, PL7_5, PL6_1, PL6, PL7, PL7_5, PL17, PL17_2, PL7, PL7
PUL0313 microarray, Northern Blot, RT-qPCR, electrophoretic mobility shift assay, clone and expression, gene deletion mutant and growth assay alginate Zobellia galactanivorans 28983288, 32585009
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017. Nucleic Acids Res. 2020 Aug 20;48(14):7786-7800. doi: 10.1093/nar/gkaa533.
2017,2020 Aug 20 degradation 3 3 PL6, PL6_1, PL7_5, PL7, PL6, PL6_1
PUL0314 microarray laminarin, alginate Zobellia galactanivorans 28983288
Gene Expression Analysis of Zobellia galactanivorans during the Degradation of Algal Polysaccharides Reveals both Substrate-Specific and Shared Transcriptome-Wide Responses. Front Microbiol. 2017 Sep 21;8:1808. doi: 10.3389/fmicb.2017.01808. eCollection 2017.
2017 degradation 5 2 GT2, GT4
PUL0400 RT-qPCR, RNA-Seq alginate Alteromonas macleodii 25847866, 30116038
Different utilization of alginate and other algal polysaccharides by marine Alteromonas macleodii ecotypes. Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. Environ Microbiol. 2015 Oct;17(10):3857-68. doi: 10.1111/1462-2920.12862. Epub 2015 May 8. ISME J. 2019 Jan;13(1):92-103. doi: 10.1038/s41396-018-0252-4. Epub 2018 Aug 16.
2015 Oct,2019 Jan degradation 14 5 PL6_1, PL6, PL17, PL17_2, CBM32, PL7_5, PL6_3, PL7_5
PUL0445 recombinant protein expression, thin layer chromatography, enzyme activity assay alginate Sphingomonas sp. 10913091
Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate. J Bacteriol. 2000 Aug;182(16):4572-7. doi: 10.1128/JB.182.16.4572-4577.2000.
2000 Aug degradation 8 2 PL7, PL5, PL15_1
PUL0572 enzyme activity assay alginate Pseudomonas aeruginosa 8335634
Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli. J Bacteriol. 1993 Aug;175(15):4780-9. doi: 10.1128/jb.175.15.4780-4789.1993.
1993 Aug biosynthesis 12 1 PL5, PL5_1
PUL0631 growth assay, sequence homology analysis alginate Pseudooceanicola algae Lw-13e 33310406
Pseudooceanicola algae sp. nov., isolated from the marine macroalga Fucus spiralis, shows genomic and physiological adaptations for an algae-associated lifestyle. Syst Appl Microbiol. 2021 Jan;44(1):126166. doi: 10.1016/j.syapm.2020.126166. Epub 2020 Nov 27.
2021 Jan degradation 8 1 PL15, PL15_1
PUL0654 sequence homology analysis alginate Maribacter dokdonensis 62-1 33912144
CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.
2021 degradation 38 8 GH144, GH144, CE0, CE1, CBM6, GH3, PL6_1, PL6, PL12, PL6_1, PL6, PL17, PL17_2
PUL0655 sequence homology analysis alginate Maribacter dokdonensis 62-1 33912144
CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain. Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.
2021 degradation 10 2 PL7, PL7