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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0655

PubMed

33912144, Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.

Characterization method

sequence homology analysis

Genomic accession number

NZ_CAJQES010000017.1

Nucelotide position range

10692-25936

Substrate

alginate

Loci

KCP55_RS09490-KCP55_RS09545

Species

Maribacter dokdonensis 62-1/320912

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- KCP55_RS09490 WP_210707579.1 0 - 939 (+) NZ_CAJQES010000017.1:10692-11631 -
- KCP55_RS09495 WP_210707580.1 935 - 2150 (+) NZ_CAJQES010000017.1:11627-12842 6.3.2.-
- KCP55_RS09505 WP_210707581.1 3158 - 6263 (+) NZ_CAJQES010000017.1:13850-16955 -
- KCP55_RS09510 WP_071340228.1 6282 - 7731 (+) NZ_CAJQES010000017.1:16974-18423 -
- KCP55_RS09515 WP_232231312.1 7786 - 9655 (+) NZ_CAJQES010000017.1:18478-20347 -
- KCP55_RS09520 WP_244745189.1 9705 - 10581 (+) NZ_CAJQES010000017.1:20397-21273 -
- KCP55_RS09525 WP_244745192.1 10590 - 11562 (+) NZ_CAJQES010000017.1:21282-22254 -
- KCP55_RS09530 WP_210707583.1 11747 - 12449 (+) NZ_CAJQES010000017.1:22439-23141 -
- KCP55_RS09535 WP_091601973.1 12538 - 13801 (+) NZ_CAJQES010000017.1:23230-24493 -
murF KCP55_RS09545 WP_210707584.1 13967 - 15245 (-) NZ_CAJQES010000017.1:24659-25937 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 939 (+) CDS No
- 936 - 2150 (+) CDS No
- 3159 - 6263 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 6283 - 7731 (+) other Yes
- 7787 - 9655 (+) other Yes
- 9706 - 10581 (+) CAZyme: PL7| PL7 Yes
- 10591 - 11562 (+) CAZyme: PL7| PL7 Yes
- 11748 - 12449 (+) TF: DBD-Pfam|GntR,DBD-SUPERFAMILY|0039384 No
- 12539 - 13801 (+) CDS No
murF 13968 - 15245 (-) CDS No

PUL ID

PUL0655

PubMed

33912144, Front Microbiol. 2021 Apr 12;12:628055. doi: 10.3389/fmicb.2021.628055. eCollection 2021.

Title

CAZymes in Maribacter dokdonensis 62-1 From the Patagonian Shelf: Genomics and Physiology Compared to Related Flavobacteria and a Co-occurring Alteromonas Strain.

Author

Wolter LA, Mitulla M, Kalem J, Daniel R, Simon M, Wietz M

Abstract

Carbohydrate-active enzymes (CAZymes) are an important feature of bacteria in productive marine systems such as continental shelves, where phytoplankton and macroalgae produce diverse polysaccharides. We herein describe Maribacter dokdonensis 62-1, a novel strain of this flavobacterial species, isolated from alginate-supplemented seawater collected at the Patagonian continental shelf. M. dokdonensis 62-1 harbors a diverse array of CAZymes in multiple polysaccharide utilization loci (PUL). Two PUL encoding polysaccharide lyases from families 6, 7, 12, and 17 allow substantial growth with alginate as sole carbon source, with simultaneous utilization of mannuronate and guluronate as demonstrated by HPLC. Furthermore, strain 62-1 harbors a mixed-feature PUL encoding both ulvan- and fucoidan-targeting CAZymes. Core-genome phylogeny and pangenome analysis revealed variable occurrence of these PUL in related Maribacter and Zobellia strains, indicating specialization to certain "polysaccharide niches." Furthermore, lineage- and strain-specific genomic signatures for exopolysaccharide synthesis possibly mediate distinct strategies for surface attachment and host interaction. The wide detection of CAZyme homologs in algae-derived metagenomes suggests global occurrence in algal holobionts, supported by sharing multiple adaptive features with the hydrolytic model flavobacterium Zobellia galactanivorans. Comparison with Alteromonas sp. 76-1 isolated from the same seawater sample revealed that these co-occurring strains target similar polysaccharides but with different genomic repertoires, coincident with differing growth behavior on alginate that might mediate ecological specialization. Altogether, our study contributes to the perception of Maribacter as versatile flavobacterial polysaccharide degrader, with implications for biogeochemical cycles, niche specialization and bacteria-algae interactions in the oceans.