29795267, Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.

Characterization method

sequence homology analysis

Genomic accession number


Nucelotide position range







Cellulophaga lytica/979

Degradation or Biosynthesis


Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- Celly_0292 ADY28127.1 0 - 951 (-) CP002534.1:303130-304081 -
- Celly_0293 ADY28128.1 1107 - 1872 (+) CP002534.1:304237-305002
- Celly_0294 ADY28129.1 2305 - 4633 (+) CP002534.1:305435-307763
- Celly_0295 ADY28130.1 4636 - 6901 (+) CP002534.1:307766-310031 -
- Celly_0296 ADY28131.1 7249 - 10351 (+) CP002534.1:310379-313481 -
- Celly_0297 ADY28132.1 10376 - 11822 (+) CP002534.1:313506-314952 -

Cluster number


Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 951 (-) TC: gnl|TC-DB|P39836|2.A.28.3.4 Yes
- 1108 - 1872 (+) other Yes
- 2306 - 4633 (+) CAZyme: PL6_1|PL6 Yes
- 4637 - 6901 (+) CAZyme: PL17_2|PL17 Yes
- 7250 - 10351 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 10377 - 11822 (+) TC: gnl|TC-DB|C6Y217|8.A.46.1.3 Yes




29795267, Sci Rep. 2018 May 23;8(1):8075. doi: 10.1038/s41598-018-26104-1.


Ancient acquisition of "alginate utilization loci" by human gut microbiota.


Mathieu S, Touvrey-Loiodice M, Poulet L, Drouillard S, Vincentelli R, Henrissat B, Skjak-Braek G, Helbert W


In bacteria from the phylum Bacteroidetes, the genes coding for enzymes involved in polysaccharide degradation are often colocalized and coregulated in so-called "polysaccharide utilization loci" (PULs). PULs dedicated to the degradation of marine polysaccharides (e.g. laminaran, ulvan, alginate and porphyran) have been characterized in marine bacteria. Interestingly, the gut microbiome of Japanese individuals acquired, by lateral transfer from marine bacteria, the genes involved in the breakdown of porphyran, the cell wall polysaccharide of the red seaweed used in maki. Sequence similarity analyses predict that the human gut microbiome also encodes enzymes for the degradation of alginate, the main cell wall polysaccharide of brown algae. We undertook the functional characterization of diverse polysaccharide lyases from family PL17, frequently found in marine bacteria as well as those of human gut bacteria. We demonstrate here that this family is polyspecific. Our phylogenetic analysis of family PL17 reveals that all alginate lyases, which have all the same specificity and mode of action, cluster together in a very distinct subfamily. The alginate lyases found in human gut bacteria group together in a single clade which is rooted deeply in the PL17 tree. These enzymes were found in PULs containing PL6 enzymes, which also clustered together in the phylogenetic tree of PL6. Together, biochemical and bioinformatics analyses suggest that acquisition of this system appears ancient and, because only traces of two successful transfers were detected upon inspection of PL6 and PL17 families, the pace of acquisition of marine polysaccharide degradation system is probably very slow.