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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0631

PubMed

33310406, Syst Appl Microbiol. 2021 Jan;44(1):126166. doi: 10.1016/j.syapm.2020.126166. Epub 2020 Nov 27.

Characterization method

growth assay,sequence homology analysis

Genomic accession number

NZ_CP060437.1

Nucelotide position range

182863-192114

Substrate

alginate

Loci

PSAL_RS18385-PSAL_RS18420

Species

Pseudooceanicola algae Lw-13e/1537215

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- PSAL_RS18385 WP_119840551.1 0 - 942 (-) NZ_CP060437.1:182863-183805 -
- PSAL_RS18390 WP_119840421.1 1088 - 1400 (+) NZ_CP060437.1:183951-184263 -
- PSAL_RS18395 WP_119840420.1 1482 - 2226 (-) NZ_CP060437.1:184345-185089 1.1.1.-
- PSAL_RS18400 WP_119840419.1 2241 - 4593 (-) NZ_CP060437.1:185104-187456 -
- PSAL_RS18405 WP_231388708.1 4666 - 6235 (-) NZ_CP060437.1:187529-189098 -
- PSAL_RS18410 WP_119840417.1 6261 - 7143 (-) NZ_CP060437.1:189124-190006 -
- PSAL_RS18415 WP_119840416.1 7156 - 8152 (-) NZ_CP060437.1:190019-191015 -
ugpC PSAL_RS18420 WP_119840415.1 8148 - 9252 (-) NZ_CP060437.1:191011-192115 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 942 (-) STP: STP|PfkB No
- 1089 - 1400 (+) STP: STP|AraC_binding No
- 1483 - 2226 (-) CDS No
- 2242 - 4593 (-) CAZyme: PL15_1| PL15_1 Yes
- 4667 - 6235 (-) TC: gnl|TC-DB|Q9KWT6|3.A.1.1.10 Yes
- 6262 - 7143 (-) TC: gnl|TC-DB|Q9KWT7|3.A.1.1.10 Yes
- 7157 - 8152 (-) TC: gnl|TC-DB|Q9KWT8|3.A.1.1.10 Yes
ugpC 8149 - 9252 (-) TC: gnl|TC-DB|Q9KWT9|3.A.1.1.10 Yes

PUL ID

PUL0631

PubMed

33310406, Syst Appl Microbiol. 2021 Jan;44(1):126166. doi: 10.1016/j.syapm.2020.126166. Epub 2020 Nov 27.

Title

Pseudooceanicola algae sp. nov., isolated from the marine macroalga Fucus spiralis, shows genomic and physiological adaptations for an algae-associated lifestyle.

Author

Wolter LA, Wietz M, Ziesche L, Breider S, Leinberger J, Poehlein A, Daniel R, Schulz S, Brinkhoff T

Abstract

The genus Pseudooceanicola from the alphaproteobacterial Roseobacter group currently includes ten validated species. We herein describe strain Lw-13e(T), the first Pseudooceanicola species from marine macroalgae, isolated from the brown alga Fucus spiralis abundant at European and North American coasts. Physiological and pangenome analyses of Lw-13e(T) showed corresponding adaptive features. Adaptations to the tidal environment include a broad salinity tolerance, degradation of macroalgae-derived substrates (mannitol, mannose, proline), and resistance to several antibiotics and heavy metals. Notably, Lw-13e(T) can degrade oligomeric alginate via PL15 alginate lyase encoded in a polysaccharide utilization locus (PUL), rarely described for roseobacters to date. Plasmid localization of the PUL strengthens the importance of mobile genetic elements for evolutionary adaptations within the Roseobacter group. PL15 homologs were primarily detected in marine plant-associated metagenomes from coastal environments but not in the open ocean, corroborating its adaptive role in algae-rich habitats. Exceptional is the tolerance of Lw-13e(T) against the broad-spectrum antibiotic tropodithietic acid, produced by Phaeobacter spp. co-occurring in coastal habitats. Furthermore, Lw-13e(T) exhibits features resembling terrestrial plant-bacteria associations, i.e. biosynthesis of siderophores, terpenes and volatiles, which may contribute to mutual bacteria-algae interactions. Closest described relative of Lw-13e(T) is Pseudopuniceibacterium sediminis CY03(T) with 98.4% 16S rRNA gene sequence similarity. However, protein sequence-based core genome phylogeny and average nucleotide identity indicate affiliation of Lw-13e(T) with the genus Pseudooceanicola. Based on phylogenetic, physiological and (chemo)taxonomic distinctions, we propose strain Lw-13e(T) (=DSM 29013(T)=LMG 30557(T)) as a novel species with the name Pseudooceanicola algae.