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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0621

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Characterization method

RNA-Seq

Genomic accession number

JABKKE010000002.1

Nucelotide position range

129359-150740

Substrate

pectin

Loci

HPS55_02230-HPS55_02270

Species

Prevotella sp. PROD/2736291

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- HPS55_02230 NPE13157.1 0 - 3237 (+) JABKKE010000002.1:129359-132596 -
- HPS55_02235 NPE13158.1 3258 - 5190 (+) JABKKE010000002.1:132617-134549 -
- HPS55_02240 NPE13159.1 5208 - 6807 (+) JABKKE010000002.1:134567-136166 -
- HPS55_02245 - 7098 - 8568 (+) JABKKE010000002.1:136457-137927 -
- HPS55_02250 NPE13160.1 9336 - 11919 (+) JABKKE010000002.1:138695-141278 -
- HPS55_02255 NPE13161.1 12132 - 16029 (-) JABKKE010000002.1:141491-145388 -
- HPS55_02260 NPE13162.1 16670 - 18074 (-) JABKKE010000002.1:146029-147433 -
- HPS55_02265 NPE13163.1 18148 - 19417 (-) JABKKE010000002.1:147507-148776 -
- HPS55_02270 NPE13164.1 19432 - 21382 (-) JABKKE010000002.1:148791-150741 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 3237 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 3259 - 5190 (+) other Yes
- 5209 - 6807 (+) other Yes
- 9337 - 11919 (+) CAZyme: GH3| GH3 Yes
- 12133 - 16029 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 16671 - 18074 (-) CAZyme: GH57|GH57 Yes
- 18149 - 19417 (-) CAZyme: GT4|GT4 Yes
- 19433 - 21382 (-) CAZyme: GH133|GH133 Yes

PUL ID

PUL0621

PubMed

33113351, Cell Host Microbe. 2020 Dec 9;28(6):838-852.e6. doi: 10.1016/j.chom.2020.09.012. Epub 2020 Oct 27.

Title

Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Author

Galvez EJC, Iljazovic A, Amend L, Lesker TR, Renault T, Thiemann S, Hao L, Roy U, Gronow A, Charpentier E, Strowig T

Abstract

Prevotella spp. are a dominant bacterial genus within the human gut. Multiple Prevotella spp. co-exist in some individuals, particularly those consuming plant-based diets. Additionally, Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates. To investigate the relationship between Prevotella competition and diet, we isolated Prevotella species from the mouse gut, analyzed their genomes and transcriptomes in vivo, and performed competition experiments between species in mice. Diverse dominant Prevotella species compete for similar metabolic niches in vivo, which is linked to the upregulation of specific polysaccharide utilization loci (PULs). Complex plant-derived polysaccharides are required for Prevotella spp. expansion, with arabinoxylans having a prominent impact on species abundance. The most dominant Prevotella species encodes a specific tandem-repeat trsusC/D PUL that enables arabinoxylan utilization and is conserved in human Prevotella copri strains, particularly among those consuming a vegan diet. These findings suggest that efficient (arabino)xylan-utilization is a factor contributing to Prevotella dominance.