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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0012

PubMed

28683122, PLoS Genet. 2017 Jul 6;13(7):e1006877. doi: 10.1371/journal.pgen.1006877. eCollection 2017 Jul.

Characterization method

enzyme activity assay

Genomic accession number

NC_002505.1

Nucelotide position range

642814-661623

Substrate

chitin

Loci

VC_RS03095-VC_RS03145

Species

Vibrio cholerae/666

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- VC_RS03095 WP_000589191.1 0 - 1413 (-) NC_002505.1:642814-644227 -
- VC_RS03100 WP_000873677.1 1535 - 3941 (-) NC_002505.1:644349-646755 -
- VC_RS03105 WP_000122112.1 4059 - 5973 (-) NC_002505.1:646873-648787 -
- VC_RS03110 WP_001110418.1 5969 - 6854 (-) NC_002505.1:648783-649668 -
- VC_RS03115 WP_000925616.1 6850 - 8575 (-) NC_002505.1:649664-651389 -
- VC_RS03120 WP_000042558.1 8631 - 9627 (-) NC_002505.1:651445-652441 -
- VC_RS03125 WP_000211039.1 9669 - 10653 (-) NC_002505.1:652483-653467 -
- VC_RS03130 WP_000646270.1 10655 - 11681 (-) NC_002505.1:653469-654495 -
- VC_RS03135 WP_000540337.1 11683 - 12670 (-) NC_002505.1:654497-655484 -
- VC_RS03140 WP_000880698.1 12839 - 14510 (-) NC_002505.1:655653-657324 -
- VC_RS03145 WP_000488279.1 15420 - 18810 (-) NC_002505.1:658234-661624 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1413 (-) CDS No
- 1536 - 3941 (-) CAZyme: GH94 Yes
- 4060 - 5973 (-) CAZyme: GH20 Yes
- 5970 - 6854 (-) other Yes
- 6851 - 8575 (-) CAZyme: GH9 Yes
- 8632 - 9627 (-) TC: gnl|TC-DB|Q97UG5|3.A.1.5.8 Yes
- 9670 - 10653 (-) TC: gnl|TC-DB|Q9X271|3.A.1.5.15 Yes
- 10656 - 11681 (-) TC: gnl|TC-DB|Q9X0U8|3.A.1.5.14 Yes
- 11684 - 12670 (-) TC: gnl|TC-DB|Q9WXN7|3.A.1.5.16 Yes
- 12840 - 14510 (-) TC: gnl|TC-DB|Q9WXS6|3.A.1.5.13 Yes
- 15421 - 18810 (-) STP: STP|HisKA,STP|HATPase_c,STP|Response_reg,STP|PAS No

PUL ID

PUL0012

PubMed

28683122, PLoS Genet. 2017 Jul 6;13(7):e1006877. doi: 10.1371/journal.pgen.1006877. eCollection 2017 Jul.

Title

The nucleoid occlusion protein SlmA is a direct transcriptional activator of chitobiose utilization in Vibrio cholerae.

Author

Klancher CA, Hayes CA, Dalia AB

Abstract

Chitin utilization by the cholera pathogen Vibrio cholerae is required for its persistence and evolution via horizontal gene transfer in the marine environment. Genes involved in the uptake and catabolism of the chitin disaccharide chitobiose are encoded by the chb operon. The orphan sensor kinase ChiS is critical for regulation of this locus, however, the mechanisms downstream of ChiS activation that result in expression of the chb operon are poorly understood. Using an unbiased transposon mutant screen, we uncover that the nucleoid occlusion protein SlmA is a regulator of the chb operon. SlmA has not previously been implicated in gene regulation. Also, SlmA is a member of the TetR family of proteins, which are generally transcriptional repressors. In vitro, we find that SlmA binds directly to the chb operon promoter, and in vivo, we show that this interaction is required for transcriptional activation of this locus and for chitobiose utilization. Using point mutations that disrupt distinct functions of SlmA, we find that DNA-binding, but not nucleoid occlusion, is critical for transcriptional activation. This study identifies a novel role for SlmA as a transcriptional regulator in V. cholerae in addition to its established role as a cell division licensing factor.