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PlantCAZyme
Cite: NAR/gkaa742 2020

Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL General Information

Literature Information

PUL ID

PUL0003

PubMed

26559526, Appl Microbiol Biotechnol. 2016 Feb;100(3):1501-1510. doi: 10.1007/s00253-015-7124-x. Epub 2015 Nov 12.

Characterization method

RT-PCR

Genomic accession number

NC_000964

Nucelotide position range

1942714-1945654

Substrate

xylan

Loci

xynCD

Species

Bacillus subtilis/1423

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

xynC BSU_18150 NP_389697.1 0 - 1269 (-) NC_000964.3:1942714-1943983 3.2.1.136
xynD BSU_18160 NP_389698.1 1399 - 2941 (-) NC_000964.3:1944113-1945655 3.2.1.55

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

xynC 1 - 1269 (-) CAZyme: GH30|GH30_8 No
xynD 1400 - 2941 (-) CAZyme: GH43_16|CBM6 No

PUL ID

PUL0003

PubMed

26559526, Appl Microbiol Biotechnol. 2016 Feb;100(3):1501-1510. doi: 10.1007/s00253-015-7124-x. Epub 2015 Nov 12.

Title

Metabolic potential of Bacillus subtilis 168 for the direct conversion of xylans to fermentation products.

Author

Rhee MS, Wei L, Sawhney N, Kim YS, Rice JD, Preston JF

Abstract

Methylglucuronoxylans (MeGXn) and methylglucuronoarabinoxylans (MeGAXn) respectively comprise most of the hemicellulose fractions in dicots and monocots and, next to cellulose, are the major resources for the production of fuels and chemicals from lignocellulosics. With either MeGXn or MeGAXn as a substrate, Bacillus subtilis 168 accumulates acidic methylglucuronoxylotriose as a limit product following the uptake and metabolism of neutral xylooligosaccharides. Secreted GH11 endoxylanase (Xyn11A), GH30 endoxylanase (Xyn30C), and GH43 arabinoxylan arabinofuranohydrolase (Axh43) respectively encoded by the xynA, xynC, and xynD genes collectively contribute to the depolymerization of MeGAXn. Studies here demonstrate the complementary roles of these enzymes in the digestion of MeGAXn. Coordinate expression of the xynD and xynC genes defines an operon accounting for the Axh43-catalyzed release of arabinose followed by Xyn30C and Xyn11A-catalyzed depolymerization of MeGAXn. Both sources generate acetate and lactate as the principal fermentation products, with yields of 26 % acetate and 32 % lactate from MeGXn compared to 22 % acetate and 21 % lactate from MeGAXn. These studies of the GH43/GH30/GH11 system in B. subtilis 168 provide a basis for the further development of B. subtilis and related species as biocatalysts for direct conversion of hemicellulose derived from energy crops as well as agricultural and forest residues to chemical feedstocks.