PUL ID

PUL0018

PubMed

22311922, Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.

Characterization method

rapid plate method growth assay,gene deletion mutant and growth assay,RT-PCR,enzyme activity assay

Genomic accession number

NC_003098.1

Nucelotide position range

285103-298853

Substrate

glycosaminoglycan

Loci

SPR_RS01490_SPR_RS01555

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 33 - 3237 (+) CAZyme: CBM70|PL8_1|PL8 Yes
- 4381 - 5010 (-) other Yes
- 5020 - 6021 (-) STP: STP|PfkB Yes
- 6052 - 6693 (-) other Yes
- 6712 - 7527 (-) other Yes
- 7798 - 8232 (+) TC: gnl|TC-DB|Q8DR78|4.A.6.1.14 Yes
- 8244 - 9434 (+) CAZyme: GH88 Yes
- 9445 - 9936 (+) TC: gnl|TC-DB|Q8DR76|4.A.6.1.14 Yes
- 9951 - 10730 (+) TC: gnl|TC-DB|Q8DR75|4.A.6.1.14 Yes
- 10717 - 11535 (+) TC: gnl|TC-DB|Q8DR74|4.A.6.1.14 Yes
- 11535 - 11828 (+) other Yes
- 11850 - 13751 (+) CAZyme: PL12_1 Yes

PUL ID

PUL0018

PubMed

22311922, Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.

Title

Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth.

Author

Marion C, Stewart JM, Tazi MF, Burnaugh AM, Linke CM, Woodiga SA, King SJ

Abstract

The mechanisms by which Streptococcus pneumoniae obtains carbohydrates for growth during airway colonization remain to be elucidated. The low concentration of free carbohydrates in the normal human airway suggests that pneumococci must utilize complex glycan structures for growth. The glycosaminoglycan hyaluronic acid is present on the apical surface of airway epithelial cells. As pneumococci express a hyaluronate lyase (Hyl) that cleaves hyaluronic acid into disaccharides, we hypothesized that during colonization pneumococci utilize the released carbohydrates for growth. Hyaluronic acid supported significant pneumococcal growth in an hyl-dependent manner. A phosphoenolpyruvate-dependent phosphotransferase system (PTS) and an unsaturated glucuronyl hydrolase (Ugl) encoded downstream of hyl are also essential for growth on hyaluronic acid. This genomic arrangement is present in several other organisms, suggesting conservation of the utilization mechanism between species. In vivo experiments support the hypothesis that S. pneumoniae utilizes hyaluronic acid as a carbon source during colonization. We also demonstrate that pneumococci can utilize the hyaluronic acid capsule of other bacterial species for growth, suggesting an alternative carbohydrate source for pneumococcal growth. Together, these data support a novel function for pneumococcal degradation of hyaluronic acid in vivo and provide mechanistic details of growth on this glycosaminoglycan.