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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0018

PubMed

22311922, Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.

Characterization method

rapid plate method growth assay,gene deletion mutant and growth assay,RT-PCR,enzyme activity assay

Genomic accession number

NC_003098.1

Nucelotide position range

285103-298853

Substrate

glycosaminoglycan

Loci

SPR_RS01490_SPR_RS01555

Species

Streptococcus pneumoniae/1313

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- SPR_RS01490 WP_001193721.1 33 - 3237 (+) NC_003098.1:285136-288340 -
- SPR_RS01505 WP_000167287.1 4380 - 5010 (-) NC_003098.1:289483-290113 -
- SPR_RS01510 WP_000161491.1 5019 - 6021 (-) NC_003098.1:290122-291124 -
- SPR_RS01515 WP_000684777.1 6051 - 6693 (-) NC_003098.1:291154-291796 -
- SPR_RS01520 WP_000185874.1 6711 - 7527 (-) NC_003098.1:291814-292630 1.1.1.69
- SPR_RS01525 WP_000706826.1 7797 - 8232 (+) NC_003098.1:292900-293335 -
- SPR_RS01530 WP_000592948.1 8243 - 9434 (+) NC_003098.1:293346-294537 -
- SPR_RS01535 WP_000178620.1 9444 - 9936 (+) NC_003098.1:294547-295039 -
- SPR_RS01540 WP_000026610.1 9950 - 10730 (+) NC_003098.1:295053-295833 -
- SPR_RS01545 WP_000148019.1 10716 - 11535 (+) NC_003098.1:295819-296638 -
- SPR_RS01550 WP_000381739.1 11534 - 11828 (+) NC_003098.1:296637-296931 -
- SPR_RS01555 WP_000657079.1 11849 - 13751 (+) NC_003098.1:296952-298854 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 33 - 3237 (+) CAZyme: CBM70|PL8_1|PL8 Yes
- 4381 - 5010 (-) other Yes
- 5020 - 6021 (-) STP: STP|PfkB Yes
- 6052 - 6693 (-) other Yes
- 6712 - 7527 (-) other Yes
- 7798 - 8232 (+) TC: gnl|TC-DB|Q8DR78|4.A.6.1.14 Yes
- 8244 - 9434 (+) CAZyme: GH88 Yes
- 9445 - 9936 (+) TC: gnl|TC-DB|Q8DR76|4.A.6.1.14 Yes
- 9951 - 10730 (+) TC: gnl|TC-DB|Q8DR75|4.A.6.1.14 Yes
- 10717 - 11535 (+) TC: gnl|TC-DB|Q8DR74|4.A.6.1.14 Yes
- 11535 - 11828 (+) other Yes
- 11850 - 13751 (+) CAZyme: PL12_1 Yes

PUL ID

PUL0018

PubMed

22311922, Infect Immun. 2012 Apr;80(4):1390-8. doi: 10.1128/IAI.05756-11. Epub 2012 Feb 6.

Title

Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth.

Author

Marion C, Stewart JM, Tazi MF, Burnaugh AM, Linke CM, Woodiga SA, King SJ

Abstract

The mechanisms by which Streptococcus pneumoniae obtains carbohydrates for growth during airway colonization remain to be elucidated. The low concentration of free carbohydrates in the normal human airway suggests that pneumococci must utilize complex glycan structures for growth. The glycosaminoglycan hyaluronic acid is present on the apical surface of airway epithelial cells. As pneumococci express a hyaluronate lyase (Hyl) that cleaves hyaluronic acid into disaccharides, we hypothesized that during colonization pneumococci utilize the released carbohydrates for growth. Hyaluronic acid supported significant pneumococcal growth in an hyl-dependent manner. A phosphoenolpyruvate-dependent phosphotransferase system (PTS) and an unsaturated glucuronyl hydrolase (Ugl) encoded downstream of hyl are also essential for growth on hyaluronic acid. This genomic arrangement is present in several other organisms, suggesting conservation of the utilization mechanism between species. In vivo experiments support the hypothesis that S. pneumoniae utilizes hyaluronic acid as a carbon source during colonization. We also demonstrate that pneumococci can utilize the hyaluronic acid capsule of other bacterial species for growth, suggesting an alternative carbohydrate source for pneumococcal growth. Together, these data support a novel function for pneumococcal degradation of hyaluronic acid in vivo and provide mechanistic details of growth on this glycosaminoglycan.