PUL ID

PUL0026

PubMed

31901520, Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.

Characterization method

qPCR, Western Blot, RNA-Seq, enzyme activity assay

Genomic accession number

AE015928.1

Nucelotide position range

3477149-3489799

Substrate

ribose

Loci

BT2802-BT2809

Species

Bacteroides thetaiotaomicron/818

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- BT_2802 AAO77908.1 0 - 1542 (+) AE015928.1:3477149-3478691 -
- BT_2803 AAO77909.1 1728 - 2694 (+) AE015928.1:3478877-3479843 -
- BT_2804 AAO77910.1 2733 - 3669 (+) AE015928.1:3479882-3480818 -
- BT_2805 AAO77911.1 3681 - 6933 (+) AE015928.1:3480830-3484082 -
- BT_2806 AAO77912.1 6945 - 8946 (+) AE015928.1:3484094-3486095 -
- BT_2807 AAO77913.1 8979 - 10599 (+) AE015928.1:3486128-3487748 -
- BT_2808 AAO77914.1 10610 - 11627 (+) AE015928.1:3487759-3488776 -
- BT_2809 AAO77915.1 11646 - 12651 (+) AE015928.1:3488795-3489800 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 1542 (+) CDS No
- 1729 - 2694 (+) STP: STP|PfkB No
- 2734 - 3669 (+) STP: STP|PfkB No
- 3682 - 6933 (+) TC: gnl|TC-DB|Q45780|1.B.14.6.1 Yes
- 6946 - 8946 (+) other Yes
- 8980 - 10599 (+) CAZyme: GH0|GH35 Yes
- 10611 - 11627 (+) CDS No
- 11647 - 12651 (+) CDS No

PUL ID

PUL0026

PubMed

31901520, Cell Host Microbe. 2020 Jan 8;27(1):79-92.e9. doi: 10.1016/j.chom.2019.11.009. Epub 2019 Dec 31.

Title

A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner.

Author

Glowacki RWP, Pudlo NA, Tuncil Y, Luis AS, Sajjakulnukit P, Terekhov AI, Lyssiotis CA, Hamaker BR, Martens EC

Abstract

Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as nucleic acids and nucleosides are less studied. We describe several ribose-utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to access ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. We used molecular genetics to probe the required functions of the system and the nature of the nutrient source(s) underlying this phenotype. Two RUS-encoded ribokinases were the only components required for this effect, presumably because they generate ribose-phosphate derivatives from products of an unlinked but essential nucleoside phosphorylase. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and the potential for unexpected dependencies among systems that mediate colonization and persistence.