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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0028

PubMed

24204617, PLoS One. 2013 Oct 25;8(10):e76341. doi: 10.1371/journal.pone.0076341. eCollection 2013.

Characterization method

microarray,qPCR,enzyme activity assay

Genomic accession number

NZ_AAYG02000020.1

Nucelotide position range

134626-149292

Substrate

mucin

Loci

RUMGNA_RS11830-RUMGNA_RS11895

Species

[Ruminococcus] gnavus/33038

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- RUMGNA_RS11830 WP_004843624.1 0 - 405 (+) NZ_AAYG02000020.1:134626-135031 -
- RUMGNA_RS11835 WP_004843625.1 531 - 1428 (-) NZ_AAYG02000020.1:135157-136054 -
- RUMGNA_RS11840 WP_024854133.1 1577 - 2495 (-) NZ_AAYG02000020.1:136203-137121 -
- RUMGNA_RS11845 WP_024854132.1 2542 - 3232 (-) NZ_AAYG02000020.1:137168-137858 -
- RUMGNA_RS11850 WP_004843631.1 3342 - 5514 (-) NZ_AAYG02000020.1:137968-140140 -
- RUMGNA_RS11855 WP_004843633.1 5510 - 6635 (-) NZ_AAYG02000020.1:140136-141261 -
- RUMGNA_RS11860 WP_039959879.1 6656 - 7490 (-) NZ_AAYG02000020.1:141282-142116 -
- RUMGNA_RS11865 WP_039959892.1 7489 - 8377 (-) NZ_AAYG02000020.1:142115-143003 -
- RUMGNA_RS11870 WP_004843638.1 8425 - 9763 (-) NZ_AAYG02000020.1:143051-144389 -
- RUMGNA_RS11875 WP_101883549.1 9914 - 10763 (-) NZ_AAYG02000020.1:144540-145389 -
- RUMGNA_RS11880 WP_004843641.1 10905 - 11355 (-) NZ_AAYG02000020.1:145531-145981 -
- RUMGNA_RS11885 WP_023923965.1 11370 - 12081 (-) NZ_AAYG02000020.1:145996-146707 -
- RUMGNA_RS11890 WP_004843644.1 12706 - 13429 (-) NZ_AAYG02000020.1:147332-148055 -
- RUMGNA_RS11895 WP_004843646.1 13413 - 13429 (-) NZ_AAYG02000020.1:148039-148055 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 405 (+) TF: DBD-Pfam|MerR,DBD-SUPERFAMILY|0042546 No
- 532 - 1428 (-) CDS No
- 1578 - 2495 (-) CDS No
- 2543 - 3232 (-) CDS No
- 3343 - 5514 (-) CAZyme: GH0|GH33|CBM40 Yes
- 5511 - 6635 (-) other Yes
- 6657 - 7490 (-) TC: gnl|TC-DB|Q72H66|3.A.1.1.25 Yes
- 7490 - 8377 (-) TC: gnl|TC-DB|Q72H67|3.A.1.1.25 Yes
- 8426 - 9763 (-) STP: STP|SBP_bac_1 Yes
- 9915 - 10763 (-) TF: DBD-Pfam|HTH_AraC,DBD-Pfam|HTH_AraC,DBD-SUPERFAMILY|0036286,DBD-SUPERFAMILY|0035607 Yes
- 10906 - 11355 (-) other Yes
- 11371 - 12081 (-) other Yes
- 12707 - 13429 (-) CAZyme: GH1 Yes
- 13414 - 13429 (-) CAZyme: GH140 Yes

PUL ID

PUL0028

PubMed

24204617, PLoS One. 2013 Oct 25;8(10):e76341. doi: 10.1371/journal.pone.0076341. eCollection 2013.

Title

Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.

Author

Crost EH, Tailford LE, Le Gall G, Fons M, Henrissat B, Juge N

Abstract

Commensal bacteria often have an especially rich source of glycan-degrading enzymes which allow them to utilize undigested carbohydrates from the food or the host. The species Ruminococcus gnavus is present in the digestive tract of >/=90% of humans and has been implicated in gut-related diseases such as inflammatory bowel diseases (IBD). Here we analysed the ability of two R. gnavus human strains, E1 and ATCC 29149, to utilize host glycans. We showed that although both strains could assimilate mucin monosaccharides, only R. gnavus ATCC 29149 was able to grow on mucin as a sole carbon source. Comparative genomic analysis of the two R. gnavus strains highlighted potential clusters and glycoside hydrolases (GHs) responsible for the breakdown and utilization of mucin-derived glycans. Transcriptomic and functional activity assays confirmed the importance of specific GH33 sialidase, and GH29 and GH95 fucosidases in the mucin utilisation pathway. Notably, we uncovered a novel pathway by which R. gnavus ATCC 29149 utilises sialic acid from sialylated substrates. Our results also demonstrated the ability of R. gnavus ATCC 29149 to produce propanol and propionate as the end products of metabolism when grown on mucin and fucosylated glycans. These new findings provide molecular insights into the strain-specificity of R. gnavus adaptation to the gut environment advancing our understanding of the role of gut commensals in health and disease.