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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0042

PubMed

30054816, J Microbiol. 2018 Sep;56(9):648-655. doi: 10.1007/s12275-018-8225-x. Epub 2018 Jul 27.

Characterization method

RT-PCR

Genomic accession number

CP001340.1

Nucelotide position range

1311323-1319129

Substrate

sucrose

Loci

SucABCD

Species

Caulobacter vibrioides/155892

Degradation or Biosynthesis

degradation

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- CCNA_01192 ACL94657.1 0 - 924 (-) CP001340.1:1311323-1312247 2.7.1.4
- CCNA_01193 ACL94658.2 905 - 2735 (-) CP001340.1:1312228-1314058 2.4.1.4
- CCNA_01194 ACL94659.1 2945 - 5399 (-) CP001340.1:1314268-1316722 -
- CCNA_01195 ACL94660.1 5549 - 6575 (-) CP001340.1:1316872-1317898 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 924 (-) other Yes
- 906 - 2735 (-) CAZyme: GH13_4 Yes
- 2946 - 5399 (-) CDS No
- 5550 - 6575 (-) TF: DBD-Pfam|LacI,DBD-SUPERFAMILY|0036955 No

PUL ID

PUL0042

PubMed

30054816, J Microbiol. 2018 Sep;56(9):648-655. doi: 10.1007/s12275-018-8225-x. Epub 2018 Jul 27.

Title

SucA-dependent uptake of sucrose across the outer membrane of Caulobacter crescentus.

Author

Modrak SK, Melin ME, Bowers LM

Abstract

Caulobacter crescentus is an aquatic Gram-negative bacterium that lives in nutrient-poor environments. Like several other aquatic and phytopathogenic bacteria, Caulobacter cells have a relatively large number of genes predicted to encode TonB-dependent receptors (TBDRs). TBDRs transport nutrients across the outer membrane using energy from the proton motive force. We identified one TBDR gene, sucA, which is situated within a cluster of genes predicted to encode a lacI-family transcription factor (sucR), amylosucrase (sucB), fructokinase (sucC), and an inner membrane transporter (sucD). Given its genomic neighborhood, we proposed that sucA encodes a transporter for sucrose. Using RT-qPCR, we determined that expression of sucABCD is strongly induced by sucrose in the media and repressed by the transcription factor, SucR. Furthermore, cells with a deletion of sucA have a reduced uptake of sucrose. Although cells with a non-polar deletion of sucA can grow with sucrose as the sole carbon source, cells with a polar deletion that eliminates expression of sucABCD cannot grow with sucrose as the sole carbon source. These results show that the suc locus is essential for sucrose utilization while SucA functions as one method of sucrose uptake in Caulobacter crescentus. This work sheds light on a new carbohydrate utilization locus in Caulobacter crescentus.