dbCAN-PUL

PUL ID	PUL0043
PubMed	29886169, Int J Biol Macromol. 2018 Oct 1;117:1195-1199. doi: 10.1016/j.ijbiomac.2018.06.029. Epub 2018 Jun 7.
Characterization method	Smith degradation,sequence homology analysis
Genomic accession number	MH306195
Nucelotide position range	5072-32981
Substrate	capsule polysaccharide
Loci	wzc-wzb-wza-gna-mnaAB-wzx-gtr198-gtr199-wzy-fnlABC-gtr31-fnr1-itrB3-atr7-gdr-galU-ugd-gpi-gne1-pgm
Species	Acinetobacter baumannii/470
Degradation or Biosynthesis	biosynthesis

Gene Name	Locus Tag	Protein ID	Gene Position	GenBank Contig Range	EC Number
wzc	-	AWU46308.1	0 - 2184 (-)	MH306195.1:5072-7256	-
wzb	-	AWU46309.1	2202 - 2577 (-)	MH306195.1:7274-7649	-
wza	-	AWU46306.1	2635 - 3736 (-)	MH306195.1:7707-8808	-
gna	-	AWU46307.1	4091 - 5366 (+)	MH306195.1:9163-10438	-
mnaA	-	AWU46310.1	5379 - 6504 (+)	MH306195.1:10451-11576	-
mnaB	-	AWU46311.1	6535 - 7795 (+)	MH306195.1:11607-12867	-
wzx	-	AWU46312.1	7836 - 8970 (+)	MH306195.1:12908-14042	-
gtr198	-	AWU46313.1	8980 - 10018 (+)	MH306195.1:14052-15090	-
gtr199	-	AWU46314.1	10014 - 11136 (+)	MH306195.1:15086-16208	-
wzy	-	AWU46315.1	11132 - 12443 (+)	MH306195.1:16204-17515	-
fnlA	-	AWU46316.1	12460 - 13498 (+)	MH306195.1:17532-18570	-
fnlB	-	AWU46317.1	13500 - 14610 (+)	MH306195.1:18572-19682	-
fnlC	-	AWU46318.1	14640 - 15753 (+)	MH306195.1:19712-20825	-
gtr31	-	AWU46319.1	15764 - 16958 (+)	MH306195.1:20836-22030	-
fnr1	-	AWU46320.1	16959 - 17916 (+)	MH306195.1:22031-22988	-
itrB3	-	AWU46321.1	17919 - 18936 (+)	MH306195.1:22991-24008	-
atr7	-	AWU46322.1	18928 - 19462 (+)	MH306195.1:24000-24534	-
gdr	-	AWU46323.1	19674 - 21549 (+)	MH306195.1:24746-26621	-
galU	-	AWU46324.1	21560 - 22436 (+)	MH306195.1:26632-27508	-
ugd	-	AWU46325.1	22553 - 23816 (+)	MH306195.1:27625-28888	-
gpi	-	AWU46326.1	23812 - 25483 (+)	MH306195.1:28884-30555	-
gne1	-	AWU46327.1	25475 - 26492 (+)	MH306195.1:30547-31564	-
pgm	-	AWU46328.1	26539 - 27910 (-)	MH306195.1:31611-32982	-

Cluster number	1
Gene name	Gene position	Gene type	Found by CGCFinder?
-	1 - 2184 (-)	TC: gnl\|TC-DB\|P76387\|8.A.3.3.2	Yes
-	2203 - 2577 (-)	other	Yes
-	2636 - 3736 (-)	TC: gnl\|TC-DB\|P0A930\|1.B.18.3.1	Yes
-	4092 - 5366 (+)	other	Yes
-	5380 - 6504 (+)	other	Yes
-	6536 - 7795 (+)	other	Yes
-	7837 - 8970 (+)	other	Yes
-	8981 - 10018 (+)	other	Yes
-	10015 - 11136 (+)	CAZyme: GT4	Yes
-	11133 - 12443 (+)	other	Yes
-	12461 - 13498 (+)	TC: gnl\|TC-DB\|Q6MMD5\|9.B.18.2.1	Yes
-	13501 - 14610 (+)	other	Yes
-	14641 - 15753 (+)	other	Yes
-	15765 - 16958 (+)	other	Yes
-	16960 - 17916 (+)	other	Yes
-	17920 - 18936 (+)	TC: gnl\|TC-DB\|G2HLY8\|9.B.146.1.2	Yes
-	18929 - 19462 (+)	other	Yes
-	19675 - 21549 (+)	TC: gnl\|TC-DB\|Q6MMD5\|9.B.18.2.1	Yes
-	21561 - 22436 (+)	CDS	No
-	22554 - 23816 (+)	CDS	No
-	23813 - 25483 (+)	CDS	No
-	25476 - 26492 (+)	CDS	No
-	26540 - 27910 (-)	CDS	No

PUL ID	PUL0043
PubMed	29886169, Int J Biol Macromol. 2018 Oct 1;117:1195-1199. doi: 10.1016/j.ijbiomac.2018.06.029. Epub 2018 Jun 7.
Title	Structure and gene cluster of the K125 capsular polysaccharide from Acinetobacter baumannii MAR13-1452.
Author	Arbatsky NP, Shneider MM, Dmitrenok AS, Popova AV, Shagin DA, Shelenkov AA, Mikhailova YV, Edelstein MV, Knirel YA
Abstract	A capsular polysaccharide (CPS) was isolated from strain MAR13-1452 of an emerging pathogen Acinetobacter baumannii and assigned type K125. The following structure of the CPS was established by sugar analysis, Smith degradation, and 1D and 2D (1)H and (13)C NMR spectroscopy: Proteins encoded by the KL125 gene cluster in the genome of MAR13-1452, including three glycosyltransferases, were assigned roles in the biosynthesis of the K125 CPS.

Copyright 2020 © YIN LAB, UNL. All rights reserved. Designed by Catie Ausland and Jinfang Zheng. Maintained by Yanbin Yin.