Because CGCFinder predicted no CGC for this PUL, the gene cluster depicted below contains dbCAN2 and CGC signature predictions for all genes in the PUL, instead of a predicted CGC.


PUL ID

PUL0046

PubMed

29738687, Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061.

Characterization method

NMR,sequence homology analysis

Genomic accession number

AB812033.1

Nucelotide position range

1-17964

Substrate

O-antigen

Loci

BAQ01244.1-BAQ01259.1_x000D_

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

wcaM - BAQ01244.1 0 - 906 (+) AB812033.1:1-907 -
gne - BAQ01245.1 1063 - 2059 (+) AB812033.1:1064-2060 -
galF - BAQ01246.1 2301 - 3195 (+) AB812033.1:2302-3196 -
- - BAQ01247.1 3826 - 4864 (+) AB812033.1:3827-4865 -
- - BAQ01248.1 5229 - 5625 (+) AB812033.1:5230-5626 -
wzx - BAQ01249.1 5697 - 6999 (+) AB812033.1:5698-7000 -
- - BAQ01250.1 6982 - 8155 (+) AB812033.1:6983-8156 -
- - BAQ01251.1 8151 - 8958 (+) AB812033.1:8152-8959 -
wzy - BAQ01252.1 8947 - 10177 (+) AB812033.1:8948-10178 -
- - BAQ01253.1 10464 - 11187 (+) AB812033.1:10465-11188 -
- - BAQ01254.1 11231 - 12278 (+) AB812033.1:11232-12279 -
- - BAQ01255.1 12256 - 13063 (+) AB812033.1:12257-13064 -
gnd - BAQ01256.1 13311 - 14718 (+) AB812033.1:13312-14719 -
ugd - BAQ01257.1 14965 - 16132 (+) AB812033.1:14966-16133 -
wzz - BAQ01258.1 16221 - 17256 (+) AB812033.1:16222-17257 -
hisI - BAQ01259.1 17352 - 17964 (-) AB812033.1:17353-17965 -

Cluster number

0

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 906 (+) CDS No
- 1064 - 2059 (+) CDS No
- 2302 - 3195 (+) CDS No
- 3827 - 4864 (+) CDS No
- 5230 - 5625 (+) CDS No
- 5698 - 6999 (+) CDS No
- 6983 - 8155 (+) CDS No
- 8152 - 8958 (+) CDS No
- 8948 - 10177 (+) CAZyme: GT2_Glycos_transf_2|GT2 No
- 10465 - 11187 (+) CDS No
- 11232 - 12278 (+) CDS No
- 12257 - 13063 (+) CDS No
- 13312 - 14718 (+) CDS No
- 14966 - 16132 (+) CDS No
- 16222 - 17256 (+) CDS No
- 17353 - 17964 (-) CDS No

PUL ID

PUL0046

PubMed

29738687, Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061.

Title

O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.

Author

Zdorovenko EL, Wang Y, Shashkov AS, Chen T, Ovchinnikova OG, Liu B, Golomidova AK, Babenko VV, Letarov AV, Knirel YA

Abstract

Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.