dbCAN-PUL

PUL ID	PUL0046
PubMed	29738687, Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061.
Characterization method	NMR,sequence homology analysis
Genomic accession number	AB812033.1
Nucelotide position range	1-17964
Substrate	O-antigen
Loci	BAQ01244.1-BAQ01259.1_x000D_
Species	Escherichia coli/562
Degradation or Biosynthesis	biosynthesis

PUL ID	PUL0046
PubMed	29738687, Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061.
Title	O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104.
Author	Zdorovenko EL, Wang Y, Shashkov AS, Chen T, Ovchinnikova OG, Liu B, Golomidova AK, Babenko VV, Letarov AV, Knirel YA
Abstract	Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.