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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0047

PubMed

31622726, Int J Biol Macromol. 2020 Jan 1;142:609-614. doi: 10.1016/j.ijbiomac.2019.09.135. Epub 2019 Oct 14.

Characterization method

sugar utilization assay,NMR,sequence homology analysis

Genomic accession number

MN443422.1

Nucelotide position range

1-32126

Substrate

O-antigen

Loci

QFR35848.1-QFR35871.1

Species

Escherichia albertii/208962

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

- - QFR35847.1 0 - 894 (+) MN443422.1:1-895 -
- - QFR35848.1 1488 - 2994 (+) MN443422.1:1489-2995 -
- - QFR35849.1 3094 - 4312 (+) MN443422.1:3095-4313 -
- - QFR35850.1 4311 - 5499 (+) MN443422.1:4312-5500 -
- - QFR35851.1 5498 - 6719 (+) MN443422.1:5499-6720 -
- - QFR35852.1 6715 - 7567 (+) MN443422.1:6716-7568 -
- - QFR35853.1 7553 - 8660 (+) MN443422.1:7554-8661 -
- - QFR35854.1 8656 - 9850 (+) MN443422.1:8657-9851 -
- - QFR35855.1 10642 - 11077 (+) MN443422.1:10643-11078 -
- - QFR35856.1 11828 - 12017 (+) MN443422.1:11829-12018 -
- - QFR35857.1 12057 - 12453 (-) MN443422.1:12058-12454 -
- - QFR35858.1 12590 - 13259 (-) MN443422.1:12591-13260 -
- - QFR35859.1 13568 - 14975 (+) MN443422.1:13569-14976 -
- - QFR35860.1 15215 - 16382 (+) MN443422.1:15216-16383 -
- - QFR35861.1 16527 - 17505 (+) MN443422.1:16528-17506 -
- - QFR35862.1 17590 - 17752 (-) MN443422.1:17591-17753 -
- - QFR35863.1 18629 - 20045 (+) MN443422.1:18630-20046 -
- - QFR35864.1 20068 - 21445 (+) MN443422.1:20069-21446 -
- - QFR35865.1 22240 - 23455 (+) MN443422.1:22241-23456 -
- - QFR35866.1 23454 - 24732 (+) MN443422.1:23455-24733 -
- - QFR35867.1 24734 - 28376 (+) MN443422.1:24735-28377 -
- - QFR35868.1 28427 - 29585 (+) MN443422.1:28428-29586 -
- - QFR35869.1 29594 - 30710 (+) MN443422.1:29595-30711 -
- - QFR35870.1 30747 - 31356 (-) MN443422.1:30748-31357 -
- - QFR35871.1 31349 - 32126 (-) MN443422.1:31350-32127 -

Cluster number

2

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 894 (+) CDS No
- 1489 - 2994 (+) CDS No
- 3095 - 4312 (+) TC: gnl|TC-DB|P37746|2.A.66.2.1 Yes (cluster 1)
- 4312 - 5499 (+) other Yes (cluster 1)
- 5499 - 6719 (+) other Yes (cluster 1)
- 6716 - 7567 (+) CAZyme: GT4 Yes (cluster 1)
- 7554 - 8660 (+) CDS No
- 8657 - 9850 (+) CDS No
- 10643 - 11077 (+) CDS No
- 11829 - 12017 (+) CDS No
- 12058 - 12453 (-) CDS No
- 12591 - 13259 (-) CDS No
- 13569 - 14975 (+) CDS No
- 15216 - 16382 (+) CDS No
- 16528 - 17505 (+) CDS No
- 17591 - 17752 (-) CDS No
- 18630 - 20045 (+) STP: STP|AraC_binding No
- 20069 - 21445 (+) CDS No
- 22241 - 23455 (+) TC: gnl|TC-DB|Q9I3V8|3.A.1.103.6 Yes (cluster 2)
- 23455 - 24732 (+) other Yes (cluster 2)
- 24735 - 28376 (+) CAZyme: GT4 Yes (cluster 2)
- 28428 - 29585 (+) CDS No
- 29595 - 30710 (+) CDS No
- 30748 - 31356 (-) CDS No
- 31350 - 32126 (-) CDS No

PUL ID

PUL0047

PubMed

31622726, Int J Biol Macromol. 2020 Jan 1;142:609-614. doi: 10.1016/j.ijbiomac.2019.09.135. Epub 2019 Oct 14.

Title

Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway.

Author

Naumenko OI, Zheng H, Shashkov AS, Sun Y, Senchenkova SN, Bai L, Wang J, Wang H, Li Q, Knirel YA, Xiong Y

Abstract

O antigen is a polysaccharide chain of a lipopolysaccharide on the outer membrane of Gram-negative bacteria. O-antigen-based serotyping and molecular typing are widely used for epidemiological and surveillance purposes. Two polysaccharides were isolated by Sephadex G-50 gel-permeation chromatography following mild acid degradation of the lipopolysaccharide of Escherichia albertii EA046 assigned to serotype O9. The polysaccharide eluted first was considered as the O-antigen. It was composed of tetrasaccharide repeating units containing two residues of d-Man and one residue each of d-Gal and d-GlcNAc as well as glycerol phosphate. It had the following unique structure which was established by NMR spectroscopy applied to the initial and dephosphorylated polysaccharides: The polysaccharide eluted from the gel second was identified as a mannan with a --> 3)-beta-d-Manp-(1 --> 2)-alpha-d-Manp-(1 --> 2)-alpha-d-Manp-(1 --> trisaccharide repeating unit. In E. albertii EA046, two polysaccharide gene clusters were found at a chromosomal locus flanked by the conserved galF gene and the histidine synthesis operon (his). They were suggested to drive the biosynthesis of the O-antigen by the Wzy/Wzy-dependent pathway and the mannan by the Wzm/Wzt-dependent pathway. The mannan shares the structure and gene cluster with a polysaccharide isolated earlier from the lipopolysaccharide of Escherichia coli O8.