29632089, J Bacteriol. 2018 May 24;200(12):e00057-18. doi: 10.1128/JB.00057-18. Print 2018 Jun 15.

Characterization method


Genomic accession number


Nucelotide position range







Streptococcus mutans/1309

Degradation or Biosynthesis


Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

treC SMU_RS09320 WP_002262351.1 0 - 1629 (-) NC_004350.2:1910627-1912256
treP SMU_RS09325 WP_002262352.1 1654 - 3622 (-) NC_004350.2:1912281-1914249
treR SMU_RS09330 WP_002273717.1 3795 - 4509 (+) NC_004350.2:1914422-1915136 -

Cluster number


Gene name

Gene position

Gene type

Found by CGCFinder?

treC 1 - 1629 (-) CAZyme: GH13_29|GH13 Yes
treP 1655 - 3622 (-) TC: gnl|TC-DB|Q720G7|4.A.1.2.13 Yes
treR 3796 - 4509 (+) STP: STP|GntR No




29632089, J Bacteriol. 2018 May 24;200(12):e00057-18. doi: 10.1128/JB.00057-18. Print 2018 Jun 15.


Characterization of the Trehalose Utilization Operon in Streptococcus mutans Reveals that the TreR Transcriptional Regulator Is Involved in Stress Response Pathways and Toxin Production.


Baker JL, Lindsay EL, Faustoferri RC, To TT, Hendrickson EL, He X, Shi W, McLean JS, Quivey RG Jr


Streptococcus mutans, the organism most frequently associated with the development of dental caries, is able to utilize a diverse array of carbohydrates for energy metabolism. One such molecule is trehalose, a disaccharide common in human foods, which has been recently implicated in enhancing the virulence of epidemic strains of the pathogen Clostridium difficile In this study, mutants with deletions of all three genes in the putative S. mutans trehalose utilization operon were characterized, and the genes were shown to be required for wild-type levels of growth when trehalose was the only carbohydrate source provided. Interestingly, the TreR transcriptional regulator appeared to be critical for responding to oxidative stress and for mounting a protective stress tolerance response following growth at moderately acidic pH. mRNA sequencing (RNA-seq) of a treR deletion mutant suggested that in S. mutans, TreR acts as a trehalose-sensing activator of transcription of the tre operon, rather than as a repressor, as described in other species. In addition, deletion of treR caused the downregulation of a number of genes involved in genetic competence and bacteriocin production, supporting the results of a recent study linking trehalose and the S. mutans competence pathways. Finally, deletion of treR compromised the ability of S. mutans to inhibit the growth of the competing species Streptococcus gordonii and Lactococcus lactis Taking the results together, this study solidifies the role of the S. mutans tre operon in trehalose utilization and suggests novel functions for the TreR regulator, including roles in the stress response and competitive fitness.IMPORTANCES. mutans is the primary etiologic agent of dental caries, which globally is the most common chronic disease. S. mutans must be able to outcompete commensal organisms in its dental plaque niche in order to establish persistence and pathogenesis. To that end, S. mutans metabolizes a diverse array of carbohydrates to generate acid and impede its acid-sensitive neighbors. Additionally, S. mutans utilizes quorum signaling through genetic competence-associated pathways to induce production of toxins to kill its rivals. This study definitively shows that the S. mutans trehalose utilization operon is required for growth in trehalose. Furthermore, this study suggests that the S. mutans TreR transcriptional regulator has a novel role in virulence through regulation of genes involved in genetic competence and toxin production.