PUL ID

PUL0059

PubMed

29787897, Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7.

Characterization method

NMR,sequence homology analysis

Genomic accession number

GU299792

Nucelotide position range

104-15235

Substrate

O-antigen

Loci

rmlB-rmlD-rmlA-fdtC-fdtB-wzx-wegP-rmlC-wzy-wegQ-wegR

Species

Escherichia coli/562

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

rmlB - ADC54932.1 104 - 1190 (+) GU299792.1:208-1294 -
rmlD - ADC54933.1 1189 - 2089 (+) GU299792.1:1293-2193 -
rmlA - ADC54934.1 2147 - 3023 (+) GU299792.1:2251-3127 -
fdtA - ADC54935.1 3037 - 3451 (+) GU299792.1:3141-3555 -
fdtC - ADC54936.1 3509 - 3905 (+) GU299792.1:3613-4009 -
fdtB - ADC54937.1 3897 - 5004 (+) GU299792.1:4001-5108 -
wzx - ADC54938.1 5003 - 6266 (+) GU299792.1:5107-6370 -
wekP - ADC54939.1 6360 - 7332 (+) GU299792.1:6464-7436 -
rmlC - ADC54940.1 7352 - 7910 (+) GU299792.1:7456-8014 -
wzy - ADC54941.1 7979 - 8942 (+) GU299792.1:8083-9046 -
wekQ - ADC54942.1 8934 - 9822 (+) GU299792.1:9038-9926 -
wekR - ADC54943.1 9852 - 10929 (+) GU299792.1:9956-11033 -
wekS - ADC54944.1 13681 - 15235 (+) GU299792.1:13785-15339 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 105 - 1190 (+) CDS No
- 1190 - 2089 (+) CDS No
- 2148 - 3023 (+) CDS No
- 3038 - 3451 (+) CDS No
- 3510 - 3905 (+) CDS No
- 3898 - 5004 (+) CDS No
- 5004 - 6266 (+) TC: gnl|TC-DB|P0AAA7|2.A.66.2.3 Yes
- 6361 - 7332 (+) other Yes
- 7353 - 7910 (+) other Yes
- 7980 - 8942 (+) other Yes
- 8935 - 9822 (+) CAZyme: GT2|GT2_Glycos_transf_2 Yes
- 9853 - 10929 (+) CDS No
- 13682 - 15235 (+) CDS No

PUL ID

PUL0059

PubMed

29787897, Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7.

Title

Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2.

Author

Yang B, Senchenkova SN, Naumenko OI, Shashkov AS, Liu B, Perepelov AV, Knirel YA

Abstract

An O-specific polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O50 followed by gel chromatography on Sephadex G-50. The following structure of the tetrasaccharide repeat was established by sugar analysis and 1D and 2D (1)H and (13)C NMR spectroscopy: -->3)-alpha-l-Rhap-(1 --> 2)-alpha-l-Rhap-(1 --> 3)-beta-l-Rhap-(1 --> 4)-beta-d-GlcpNAc-(1--> The linear O50 polysaccharide has the same structure as the main chain of the branched O polysaccharide of E. coli O2 studied earlier [Jansson et al., Carbohydr. Res. 161 (1987) 273-279], which differs in the presence of a side-chain alpha-d-Fucp3NAc residue. In spite of the difference between the O-polysaccharides, the corresponding genes in the O2- and O50-antigen gene cluster are 99-100% identical. The genetic basis for the lack of d-Fucp3NAc from the O50 polysaccharide is evidently a point mutation in the aminotransferase gene fdtB of the d-Fucp3NAc synthesis pathway resulting in a single amino acid change from histidine in O2 to arginine in O50.