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Cite: NAR/gkaa742 2020



PUL General Information

Literature Information

PUL ID

PUL0069

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Characterization method

sugar utilization assay,NMR,sequence homology analysis

Genomic accession number

MF522813

Nucelotide position range

1-25134

Substrate

capsule polysaccharide

Loci

wzc-wzb-wza-gna-psaA-psaB-psaC-psaD-psaE-psaF-wzx-gtr37-wzy-gtr5-itrA3-galU-ugd-gpi-gne1-pgm

Species

Acinetobacter baumannii/470

Degradation or Biosynthesis

biosynthesis

Gene Name

Locus Tag

Protein ID

Gene Position

GenBank Contig Range

EC Number

fkpA - AUS94299.1 0 - 723 (-) MF522813.1:1-724 -
wzc - AUS94300.1 915 - 3096 (-) MF522813.1:916-3097 -
wzb - AUS94301.1 3114 - 3543 (-) MF522813.1:3115-3544 -
wza - AUS94302.1 3547 - 4666 (-) MF522813.1:3548-4667 -
gna - AUS94303.1 5003 - 6278 (+) MF522813.1:5004-6279 -
psaA - AUS94304.1 6324 - 7323 (+) MF522813.1:6325-7324 -
psaB - AUS94305.1 7324 - 8485 (+) MF522813.1:7325-8486 -
psaC - AUS94306.1 8487 - 9180 (+) MF522813.1:8488-9181 -
psaD - AUS94307.1 9183 - 10281 (+) MF522813.1:9184-10282 -
psaE - AUS94308.1 10274 - 10790 (+) MF522813.1:10275-10791 -
psaF - AUS94309.1 10791 - 11841 (+) MF522813.1:10792-11842 -
wzx - AUS94310.1 11843 - 13061 (+) MF522813.1:11844-13062 -
gtr37 - AUS94311.1 13072 - 14197 (+) MF522813.1:13073-14198 -
wzy - AUS94312.1 14114 - 15260 (+) MF522813.1:14115-15261 -
gtr5 - AUS94313.1 15274 - 16105 (+) MF522813.1:15275-16106 -
itrA3 - AUS94314.1 16117 - 16732 (+) MF522813.1:16118-16733 -
galU - AUS94315.1 16755 - 17631 (+) MF522813.1:16756-17632 -
ugd - AUS94316.1 17745 - 19008 (+) MF522813.1:17746-19009 -
gpi - AUS94317.1 19004 - 20675 (+) MF522813.1:19005-20676 -
gne1 - AUS94318.1 20667 - 21684 (+) MF522813.1:20668-21685 -
pgm - AUS94319.1 21727 - 23098 (-) MF522813.1:21728-23099 -
lldP - AUS94320.1 23466 - 25134 (+) MF522813.1:23467-25135 -

Cluster number

1

Gene name

Gene position

Gene type

Found by CGCFinder?

- 1 - 723 (-) CDS No
- 916 - 3096 (-) TC: gnl|TC-DB|P76387|8.A.3.3.2 Yes
- 3115 - 3543 (-) other Yes
- 3548 - 4666 (-) TC: gnl|TC-DB|P0A930|1.B.18.3.1 Yes
- 5004 - 6278 (+) other Yes
- 6325 - 7323 (+) TC: gnl|TC-DB|Q6MMD5|9.B.18.2.1 Yes
- 7325 - 8485 (+) STP: STP|Aminotran_1_2 Yes
- 8488 - 9180 (+) other Yes
- 9184 - 10281 (+) CAZyme: GT0 Yes
- 10275 - 10790 (+) other Yes
- 10792 - 11841 (+) other Yes
- 11844 - 13061 (+) other Yes
- 13073 - 14197 (+) other Yes
- 14115 - 15260 (+) other Yes
- 15275 - 16105 (+) CAZyme: GT2_Glycos_transf_2|GT2 Yes
- 16118 - 16732 (+) TC: gnl|TC-DB|H8E4X1|9.B.18.1.2 Yes
- 16756 - 17631 (+) other Yes
- 17746 - 19008 (+) other Yes
- 19005 - 20675 (+) other Yes
- 20668 - 21684 (+) other Yes
- 21728 - 23098 (-) other Yes
- 23467 - 25134 (+) TC: gnl|TC-DB|P33231|2.A.14.1.1 Yes

PUL ID

PUL0069

PubMed

30664967, Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.

Title

Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster.

Author

Kenyon JJ, Arbatsky NP, Sweeney EL, Shashkov AS, Shneider MM, Popova AV, Hall RM, Knirel YA

Abstract

A new capsular polysaccharide (CPS) biosynthesis gene cluster, KL16, was found in the genome sequence of a clinical Acinetobacter baumannii ST25 isolate, D4. The variable part of KL16 contains a module of genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (5,7-di-N-acetylpseudaminic acid, Pse5Ac7Ac), a gene encoding ItrA3 that initiates the CPS synthesis with d-GlcpNAc, and two glycosyltransferase (Gtr) genes. The K16 CPS was studied by sugar analysis and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy, and shown to be built up of linear trisaccharide repeats containing d-galactose (d-Gal), N-acetyl-d-glucosamine (d-GlcNAc), and Pse5Ac7Ac. The d-Galp residue is linked to the d-GlcpNAc initiating sugar via a beta-(1 --> 3) linkage evidently formed by a Gtr5 variant, Gtr5(K16), encoded in KL16. This reveals an altered or relaxed substrate specificity of this variant as the majority of Gtr5-type glycosyltransferases have previously been shown to form a beta-d-Galp-(1 --> 3)-d-GalpNAc linkage. The beta-Psep5Ac7Ac-(2 --> 4)-d-Galp linkage is predicted to be formed by the other glycosyltransferase, Gtr37, which does not match members of any known glycosyltransferase family.